Recombinant Saccharomyces cerevisiae Mitogen-activated protein kinase SLT2/MPK1 (SLT2)

中文名稱：

釀酒酵母SLT2重組蛋白
貨號：

CSB-YP311486SVG
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母SLT2重組蛋白
貨號：

CSB-EP311486SVG
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母SLT2重組蛋白
貨號：

CSB-EP311486SVG-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母SLT2重組蛋白
貨號：

CSB-BP311486SVG
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母SLT2重組蛋白
貨號：

CSB-MP311486SVG
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

SLT2
Uniprot No.：

Q00772
別名：

SLT2; MPK1; YHR030C; Mitogen-activated protein kinase SLT2/MPK1; MAP kinase MPK1; EC 2.7.11.24
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

full length protein
表達區域：

1-484
氨基酸序列

MADKIERHTF KVFNQDFSVD KRFQLIKEIG HGAYGIVCSA RFAEAAEDTT VAIKKVTNVF SKTLLCKRSL RELKLLRHFR GHKNITCLYD MDIVFYPDGS INGLYLYEEL MECDMHQIIK SGQPLTDAHY QSFTYQILCG LKYIHSADVL HRDLKPGNLL VNADCQLKIC DFGLARGYSE NPVENSQFLT EYVATRWYRA PEIMLSYQGY TKAIDVWSAG CILAEFLGGK PIFKGKDYVN QLNQILQVLG TPPDETLRRI GSKNVQDYIH QLGFIPKVPF VNLYPNANSQ ALDLLEQMLA FDPQKRITVD EALEHPYLSI WHDPADEPVC SEKFEFSFES VNDMEDLKQM VIQEVQDFRL FVRQPLLEEQ RQLQLQQQQQ QQQQQQQQQQ QPSDVDNGNA AASEENYPKQ MATSNSVAPQ QESFGIHSQN LPRHDADFPP RPQESMMEMR PATGNTADIP PQNDNGTLLD LEKELEFGLD RKYF
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Serine/threonine protein kinase involved in a signal transduction pathway that plays a role in yeast cell morphogenesis and cell growth. This pathway seems to start by SMP3; then involve the kinase PKC1 that may act the BCK1 kinase that then phosphorylates MKK1 and MKK2 which themselves phosphorylate the SLT2/MPK1 kinase which itself then phosphorylates and activates the transcription factor RLM1. Directly phosphorylates BCY1 upon TOR complex 1 (TORC1) inhibition.
基因功能參考文獻：
1. MPK1/SLT2 links multiple stress responses with gene expression in S.cerevisiae by phosphorylating Tyr1 of the RNAP II C terminal domain. PMID: 29220656
2. Mpk1 molecules carrying a mutation in Tyr-268 executed the entire downstream biological function, using mechanisms that do not require activation of catalysis or the catalytic Lys-54 residue.It should be emphasized that although Mpk1(Y268C/A+K54R) and Mpk1(Y268C/A+T190A+Y192F) molecules are biologically active, they rescue mpk1 and mkk1mkk2 cells less efficiently than Mpk1(Y268C/A). PMID: 27413009
3. cooperation between SAGA and SWI/SNF complexes is required for efficient transcriptional responses regulated by the yeast MAPK Slt2 PMID: 27112564
4. Slt2 phosphorylates the MAPK phosphatase Msg5 both in the N-terminal regulatory and C-terminal catalytic domains. PMID: 26786099
5. Mpk1 and the greatwall kinase pathway serve TORC1 to coordinate the phosphorylation status of Sic1 and consequently G1-S cell cycle progression with nutrient availability. PMID: 26356805
6. Authors show that in sod1, grx5, and grx3 grx4 slt2 mutants, all of which are affected by chronic oxidative stress, the overexpression of Slt2 favors vacuole fusion through a mechanism dependent on an active actin cytoskeleton. PMID: 23956390
7. Our results point to a common mechanism for the regulation of ER inheritance in which Slt2p activity at the bud tip controls the association of the ER with the actin-based cytoskeleton. PMID: 23924898
8. Slt2p is activated by different inputs in response to nutrient signals and mediates growth control through TORC1 (target of rapamycin 1 complex)-Sch9p (suppressor of cdc25) and PKA (protein kinase A) at low temperatures PMID: 22747505
9. Slt2 is required for the induction of several genes in response to arsenate exposure. PMID: 20737431
10. The authors suggest that the observed length variability may influence biological features of the Slt2 protein, allowing it to adapt swiftly in order to survive in unusual environments. PMID: 20586115
11. mechanism by which the MAP kinase Slt2 and Mlp1 control mRNA export factors during heat shock stress PMID: 20823268
12. A new role for the Slt2p MAP kinase cascade protects cells from programmed cell death through cyclin C destruction. PMID: 16387872
13. Cell integrity MAPK (mitogen-activated protein kinase) function can be provided in yeast cells by either the native Slt2(Mpk1)p of yeast or by a heterologously expressed human ERK5. PMID: 17052197
14. In this study, we demonstrate that transcriptional activation of a Gal4-Slt2p fusion is responsive to cell wall stress and requires phosphorylation of Slt2p. PMID: 17397108
15. Retrophosphorylation of MKK1 and MKK2 MAPKKs by the SLT2 MAPK in the yeast cell integrity pathway is reported. PMID: 17711850
16. The Hos2p complex is required for proper activation of the Mpk1p/Slt2p cell integrity kinase cascade. PMID: 18487345
17. Data show that phosphorylation of Ser423 specifically blocked SBF activation by preventing Mpk1 association with Swi4, revealing a novel mechanism for regulating MAPK target specificity. PMID: 19805511
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蛋白家族：

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
數據庫鏈接：

KEGG: sce:YHR030C

STRING: 4932.YHR030C