Recombinant Saccharomyces cerevisiae Mitochondrial DNA base excision repair N-glycosylase 1 (NTG1)

中文名稱：

釀酒酵母NTG1重組蛋白
貨號：

CSB-YP341224SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母NTG1重組蛋白
貨號：

CSB-EP341224SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母NTG1重組蛋白
貨號：

CSB-EP341224SVG-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母NTG1重組蛋白
貨號：

CSB-BP341224SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母NTG1重組蛋白
貨號：

CSB-MP341224SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

NTG1
Uniprot No.：

P31378
別名：

NTG1; OGG2; SCR1; YAL015C; FUN33; Endonuclease III homolog 1; EC 3.2.2.-; EC 4.2.99.18; Bifunctional DNA N-glycosylase/DNA-(apurinic or apyrimidinic site) lyase 1; DNA glycosylase/AP lyase 1; Endonuclease III-like glycosylase 1; Redoxyendonuclease 1
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

Full Length of Mature Protein
表達區(qū)域：

18-399
氨基酸序列

LVK TETGPESELL PEKRTKIKQE EVVPQPVDID WVKSLPNKQY FEWIVVRNGN VPNRWATPLD PSILVTPAST KVPYKFQETY ARMRVLRSKI LAPVDIIGGS SIPVTVASKC GISKEQISPR DYRLQVLLGV MLSSQTKDEV TAMAMLNIMR YCIDELHSEE GMTLEAVLQI NETKLDELIH SVGFHTRKAK YILSTCKILQ DQFSSDVPAT INELLGLPGV GPKMAYLTLQ KAWGKIEGIC VDVHVDRLTK LWKWVDAQKC KTPDQTRTQL QNWLPKGLWT EINGLLVGFG QIITKSRNLG DMLQFLPPDD PRSSLDWDLQ SQLYKEIQQN IMSYPKWVKY LEGKRELNVE AEINVKHEEK TVEETMVKLE NDISVKVED
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Bifunctional DNA N-glycosylase with associated apurinic/apyrimidinic (AP) lyase function that catalyzes the first step in base excision repair (BER), the primary repair pathway for the repair of oxidative DNA damage. The DNA N-glycosylase activity releases the damaged DNA base from DNA by cleaving the N-glycosidic bond, leaving an AP site. The AP-lyase activity cleaves the phosphodiester bond 3' to the AP site by a beta-elimination. Primarily recognizes and repairs oxidative base damage of pyrimidines, but also purine-derived lesions, alkylation damage and cytosine photoproducts generated by UV irradiation as well as abasic sites. Has also 8-oxoguanine DNA glycosylase activity. The AP lyase can incise AP sites opposite all four bases. May also play a role in the regulation of mtDNA copy number by introducing a double-stranded break (DSB) at the mtDNA replication origin ori5, initiating the rolling-circle mtDNA replication.
基因功能參考文獻：
1. Both Ntg1 and its human homologue, NTHL1, can be SUMO-modified in response to oxidative stress. PMID: 27839712
2. the elements necessary for nuclear and mitochondrial localization of Ntg1 were identified, including a bipartite classical nuclear localization signal, a mitochondrial matrix targeting sequence and the classical nuclear protein import machinery. PMID: 20194111
3. loss of Ntg1p suppresses mitochondrial point mutation rates, frameshifts and recombination rates PMID: 16730479
4. The superb activity of the 8-oxoguanine glycosylases Fpg, hOGG1, and Ntg1 toward removal of FapyG and FapyA in vitro suggests that these enzymes may act upon these oxidized lesions in vivo. PMID: 18154319
5. The intracellular localization of the Saccharomyces cerevisiae N-glycosylase/AP lyases, Ntg1 and Ntg2, in response to nuclear and mitochondrial oxidative stress, was determined. PMID: 19029246
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亞細胞定位：

Nucleus. Mitochondrion.
蛋白家族：

Nth/MutY family
數(shù)據(jù)庫鏈接：

KEGG: sce:YAL015C

STRING: 4932.YAL015C