Recombinant Saccharomyces cerevisiae Glutaredoxin-1 (GRX1)

中文名稱：

釀酒酵母GRX1重組蛋白
貨號：

CSB-YP009521SVG
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母GRX1重組蛋白
貨號：

CSB-EP009521SVG
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母GRX1重組蛋白
貨號：

CSB-EP009521SVG-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母GRX1重組蛋白
貨號：

CSB-BP009521SVG
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母GRX1重組蛋白
貨號：

CSB-MP009521SVG
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

GRX1
Uniprot No.：

P25373
別名：

GRX1; YCL035C; YCL35CGlutaredoxin-1; EC 1.11.1.9; EC 2.5.1.18; Glutathione-dependent oxidoreductase 1
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

full length protein
表達區域：

1-110
氨基酸序列

MVSQETIKHV KDLIAENEIF VASKTYCPYC HAALNTLFEK LKVPRSKVLV LQLNDMKEGA DIQAALYEIN GQRTVPNIYI NGKHIGGNDD LQELRETGEL EELLEPILAN
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Component of the glutathione system which performs several activities such as glutathione-dependent oxidoreductase, glutathione peroxidase and glutathione S-transferase (GST) activity. The disulfide bond functions as an electron carrier in the glutathione-dependent synthesis of deoxyribonucleotides by the enzyme ribonucleotide reductase. In addition, it is also involved in reducing cytosolic protein- and non-protein-disulfides in a coupled system with glutathione reductase. Required for resistance to reactive oxygen species (ROS) by directly reducing hydroperoxides and for the detoxification of ROS-mediated damage. GRX1 is less active as an oxidoreductase than GRX2.
基因功能參考文獻：
1. Grx1p and Grx2p protect against Selenite-induced cell death. PMID: 20522499
2. analysis of the structural basis for the different activities of yeast Grx1 and Grx2 PMID: 20417731
3. Redox-sensitive yellow fluorescent protein fused to glutaredoxin 1 (Grx1p). Intramolecular transfer of reducing equivalents made the fusion protein kinetically superior in reactions with glutathione. PMID: 16475825
4. Glutaredoxin Grx1p has been crystallized both as an independent protein and in a protein fusion with histidine-tagged yellow fluorescent protein. PMID: 16946480
5. Comparative structural analyses of the oxidized and glutathionylated Grx1 illustrate the conformational changes of residues at different redox states, whose roles during catalysis are further compared with the homologous structures. PMID: 18473363
6. We hypothesize that the substitutions of Ser23 and Gln52 in yGrx1 by Ala23 and Glu52 in yGrx2 modify the capability of the active-site C-terminal cysteine to attack the mixed disulfide between the N-terminal active-site cysteine and glutathione molecule PMID: 18992757
顯示更多

收起更多
亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

Glutaredoxin family
數據庫鏈接：

KEGG: sce:YCL035C

STRING: 4932.YCL035C