Recombinant Saccharomyces cerevisiae E3 ubiquitin-protein ligase RSP5 (RSP5), partial

中文名稱：

釀酒酵母RSP5重組蛋白
貨號：

CSB-YP334646SVG
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母RSP5重組蛋白
貨號：

CSB-EP334646SVG
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母RSP5重組蛋白
貨號：

CSB-EP334646SVG-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母RSP5重組蛋白
貨號：

CSB-BP334646SVG
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母RSP5重組蛋白
貨號：

CSB-MP334646SVG
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

RSP5
Uniprot No.：

P39940
別名：

RSP5; MDP1; NPI1; YER125W; SYGP-ORF41E3 ubiquitin-protein ligase RSP5; EC 2.3.2.26; HECT-type E3 ubiquitin transferase RSP5; Reverses SPT-phenotype protein 5
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Component of a RSP5 ubiquitin ligase complex which specifies polyubiquitination and intracellular trafficking of the general amino acid permease GAP1 as well as other cell surface proteins like GAP1, FUR4, MAL61, PMA1 and STE2. The RSP5-BUL1/2 complex is also necessary for the heat-shock element (HSE)-mediated gene expression, nitrogen starvation GLN3-dependent transcription, pressure-induced differential regulation of the two tryptophan permeases TAT1 and TAT2 and sorting efficiency into multivesicular bodies. Also acts on RBP1. Plays a role in tolerance to o-dinitrobenzene. Involved in actin cytoskeleton organization and dynamics. Ubiquitinates the LAS17-binding proteins LSB1 and PIN3/LSB2 without directing them for degradation and affects LAS17 levels in a SLA1-dependent and LSB1/2-independent manner.
基因功能參考文獻：
1. These data raised the possibility that Rsp5 enhances the function of Ena1/2/5 specifically in response to sodium acetate through an unknown mechanism other than ubiquitination of Rim8 and activation of Hog1-mediated signaling. PMID: 29106511
2. Authors present the molecular features in the methionine permease Mup1 that are required for Art1-Rsp5-mediated ubiquitination and endocytosis. PMID: 27798240
3. Rsp5 may be phosphorylated by PKA at position T761 and that this regulation is important for its localization and function. PMID: 26548973
4. subjecting yeast to proteotoxic stress, particularly thermal stress, triggers arresting related trafficking adaptor-Rsp5-mediated clearance of misfolded plasma membrane proteins. PMID: 23599894
5. Our findings suggest that stress-induced quality control is a common process requiring Rsp5p for plasma membrane proteins in yeast. PMID: 25001409
6. Rsp5 and its mammalian homologue Nedd4 are important E3 ligases responsible for the increased ubiquitylation induced by heat stress. PMID: 25344756
7. SUMOylation regulates the homologous to E6-AP carboxyl terminus (HECT) ubiquitin ligase Rsp5p. PMID: 23443663
8. In one highly sensitive cluster on the surface of ubiquitin, almost every amino acid substitution caused growth defects. PMID: 23376099
9. our results show that Rsp5 ligase plays a role in regulating retrograde Golgi-to-ER trafficking. PMID: 22761830
10. Rsp5 together with Lsb1, Lsb2 and Sla1 regulate the level of Las17, an important activator of actin polymerization. PMID: 22000681
11. Yeast ubiquitin ligase Rsp5 contains nuclear localization and export signals PMID: 21868125
12. suggest that functional Rsp5p is required to maintain the integrity of cytoplasmic ribosomes under rich nutrient conditions PMID: 21665996
13. Results support a model in which the N-lobe-binding site acts to localize and orient the distal end of the ubiquitin chain to promote conjugation of the next ubiquitin molecule. PMID: 21399621
14. Bsd2 is a three-transmembrane domains protein with a PPXY motif that binds the HECT domain ubiquitin ligase Rsp5 PMID: 14988731
15. Rsp5p plays a novel and key role in intracellular trafficking, and extend the currently very short list of substrates ubiquitylated in vivo by several different ubiquitin ligases acting cooperatively PMID: 15086787
16. Rsp5 is important for recovery from heat-induced protein synthesis arrest PMID: 15527788
17. Counteraction of PUT4 downregulation by npi1 mutation that affects cellular ubiquitination function is reported. PMID: 15973048
18. results suggest that enhanced phosphatidylinositol synthesis may account for PIS1 suppression of rsp5 defects PMID: 16363994
19. Sequential assembly of first a Bsd2/Rsp5 complex, then a Tre1/Bsd2/Rsp5 complex followed by a rearrangement of PY-WW interactions is required for the ubiquitination of Smf1. PMID: 17429078
20. identification of numerous known and novel ubiquitinated substrates of this E3 ligase PMID: 17551511
21. Rsp5 ubiquitin ligase is required for vacuolar targeting and degradation of high-affinity copper transporter Ctr1p fused to green fluorescent protein, following the endocytic downregulation pathway requiring delivery to the vacuole. PMID: 17645432
22. A combination of yeast proteome chip assays, genetic screening, and in vitro/in vivo biochemical analyses was used to identify and characterize eight novel in vivo substrates of the ubiquitinating enzyme Rsp5. PMID: 17951556
23. turnover of the low affinity P i transporters is initiated by Rsp5-mediated ubiquitination followed by internalization and degradation by the endocytic pathway. PMID: 18165238
24. lysine residues 983 and 985 contained within the carboxy-terminal domain of Mga2p120 are Rsp5p-directed Ub-conjugation sites PMID: 19061897
25. Rsp5p plays an important role in the post-transcriptional fate of mRNA under nutrient starvation. PMID: 19180642
26. Point mutations that disrupt ubiquitin binding also result in temperature-sensitive growth defects in yeast, indicating that the Rsp5 ubiquitin-binding site is important for Rsp5 function in vivo PMID: 19252184
27. inhibits tombavirus replication via p92 replication protein and replicase PMID: 19759160
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亞細胞定位：

Cytoplasm. Nucleus. Cytoplasm, cytoskeleton, actin patch.
蛋白家族：

RSP5/NEDD4 family
數據庫鏈接：

KEGG: sce:YER125W

STRING: 4932.YER125W