Recombinant Saccharomyces cerevisiae DNA-directed RNA polymerase I subunit RPA12 (RPA12)

中文名稱：

釀酒酵母RPA12重組蛋白
貨號(hào)：

CSB-YP027114SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

釀酒酵母RPA12重組蛋白
貨號(hào)：

CSB-EP027114SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

釀酒酵母RPA12重組蛋白
貨號(hào)：

CSB-EP027114SVG-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

釀酒酵母RPA12重組蛋白
貨號(hào)：

CSB-BP027114SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

釀酒酵母RPA12重組蛋白
貨號(hào)：

CSB-MP027114SVG
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

RPA12
Uniprot No.：

P32529
別名：

RPA12; RRN4; YJR063W; J1747; DNA-directed RNA polymerase I subunit RPA12; A12; A12.2; DNA-directed RNA polymerase I 13.7 kDa polypeptide
種屬：

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
蛋白長(zhǎng)度：

full length protein
表達(dá)區(qū)域：

1-125
氨基酸序列

MSVVGSLIFC LDCGDLLENP NAVLGSNVEC SQCKAIYPKS QFSNLKVVTT TADDAFPSSL RAKKSVVKTS LKKNELKDGA TIKEKCPQCG NEEMNYHTLQ LRSADEGATV FYTCTSCGYK FRTNN
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

DNA-dependent RNA polymerases catalyze the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I (Pol I) which synthesizes ribosomal RNA precursors. Besides, RNA polymerase I has intrinsic RNA cleavage activity. Proposed to contribute to the polymerase catalytic activity and form the polymerase active center together with the two largest subunits. Subunit RPA12 contributes a catalytic zinc ribbon that is required for RNA cleavage by Pol I. Involved in transcriptional termination.
基因功能參考文獻(xiàn)：
1. Study demonstrate that Rpa12p interacts with the transcription factor Msn4p and prevents its binding to the promoter of AYR1, a key enzyme involved in triacylglycerol biosynthesis and mobilization of nonpolar lipids. These results suggest that the deletion of RPA12 derepresses Msn4p, thus activating the transcription of AYR1, which leads to the accumulation of triacylglycerol in the rpa12D mutant. PMID: 27637775
亞細(xì)胞定位：

Nucleus, nucleolus.
蛋白家族：

Archaeal RpoM/eukaryotic RPA12/RPB9/RPC11 RNA polymerase family
數(shù)據(jù)庫(kù)鏈接：

KEGG: sce:YJR063W

STRING: 4932.YJR063W