Recombinant Rous sarcoma virus Gag-Pro-Pol polyprotein (gag-pro-pol)

中文名稱：

Recombinant Rous sarcoma virus Gag-Pro-Pol polyprotein(gag-pro-pol)
貨號：

CSB-YP365916RHO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Rous sarcoma virus Gag-Pro-Pol polyprotein(gag-pro-pol)
貨號：

CSB-EP365916RHO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Rous sarcoma virus Gag-Pro-Pol polyprotein(gag-pro-pol)
貨號：

CSB-EP365916RHO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Rous sarcoma virus Gag-Pro-Pol polyprotein(gag-pro-pol)
貨號：

CSB-BP365916RHO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Rous sarcoma virus Gag-Pro-Pol polyprotein(gag-pro-pol)
貨號：

CSB-MP365916RHO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

gag-pol
Uniprot No.：

P03354
別名：

gag-pol; Gag-Pol polyprotein [Cleaved into: Matrix protein p19; p2A; p2B; p10; Capsid protein p27; alternate cleaved 1; Capsid protein p27; alternate cleaved 2; Spacer peptide; SP; p3); Nucleocapsid protein p12; Protease p15; EC 3.4.23.-); Reverse transcriptase beta-subunit; RT-beta); Reverse transcriptase alpha-subunit; RT-alpha; EC 2.7.7.49; EC 2.7.7.7; EC 3.1.26.4); Integrase; IN; EC 2.7.7.-; EC 3.1.-.-; pp32); p4]
種屬：

Rous sarcoma virus (strain Prague C) (RSV-PrC)
蛋白長度：

full length protein
表達區域：

1-155
氨基酸序列

MEAVIKVISS ACKTYCGKTS PSKKEIGAML SLLQKEGLLM SPSDLYSPGS WDPITAALSQ RAMILGKSGE LKTWGLVLGA LKAAREEQVT SEQAKFWLGL GGGRVSPPGP ECIEKPATER RIDKGEEVGE TTVQRDAKMA PEETATPKTV GTSCY
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Capsid protein p27: Self-associates to form the irregular polyhedron core composed of hexamers and pentamers, that encapsulates the genomic RNA-nucleocapsid complex. Assembles as a tube in vitro.; Plays a role in the oligomerization of the Gag polyprotein and in the stabilization of the immature particle. Essential layering element during tube assembly.; Binds strongly to viral nucleic acids and promote their aggregation. Also destabilizes the nucleic acids duplexes via highly structured zinc-binding motifs.; The aspartyl protease mediates proteolytic cleavages of Gag and Gag-Pol polyproteins during or shortly after the release of the virion from the plasma membrane. Cleavages take place as an ordered, step-wise cascade to yield mature proteins. This process is called maturation. Displays maximal activity during the budding process just prior to particle release from the cell.; Catalyzes viral DNA integration into the host chromosome, by performing a series of DNA cutting and joining reactions. This recombination event is an essential step in the viral replication cycle. Has a strong preference for using the 3'-OH at the viral DNA end as a nucleophile.
基因功能參考文獻：
1. crystal structure of the three-domain integrase from Rous sarcoma virus in complex with viral and target DNAs PMID: 26887497
亞細胞定位：

[Matrix protein p19]: Virion.; [Capsid protein p27, alternate cleaved 1]: Virion.; [Capsid protein p27, alternate cleaved 2]: Virion.; [Nucleocapsid protein p12]: Virion.
數據庫鏈接：

KEGG: vg:2193432