Recombinant Rat Vesicular glutamate transporter 2 (Slc17a6), partial

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中文名稱：

大鼠Slc17a6重組蛋白
品名簡稱：

Recombinant Rat Slc17a6 protein, partial
貨號：

CSB-EP867480RA
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Slc17a6
Uniprot No.：

Q9JI12
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
來源：

E.coli
分子量：

17.1 kDa
表達區域：

499-582aa
氨基酸序列

SGEKQPWADPEETSEEKCGFIHEDELDEETGDITQNYINYGTTKSYGATSQENGGWPNGWEKKEEFVQESAQDAYSYKDRDDYS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Mediates the uptake of glutamate into synaptic vesicles at presynaptic nerve terminals of excitatory neural cells. May also mediate the transport of inorganic phosphate. Involved in the regulation of retinal hyaloid vessel regression during postnatal development. May also play a role in the endocrine glutamatergic system of other tissues such as pineal gland and pancreas.
基因功能參考文獻：
1. dual retrograde tract tracing was used in combination with fluorescence in situ hybridization (FISH) for VGLUT1 or VGLUT2 mRNA to identify the existence of VGLUT1 or VGLUT2 mRNA neurons that send collateral projections to both the thalamus and the parabrachial nucleus. PMID: 29650024
2. VGLUT2 was selectively up-regulated in ipsilateral spinal cord in a model of spared nerve injury. PMID: 27210824
3. Glutamate signaling that is mediated by VGLUT2 in the pulpal axons may be enhanced in the inflamed dental pulp, which may contribute to pulpal axon sensitization leading to hyperalgesia following inflammation. PMID: 25290694
4. These results suggest that VGLUT2 is localized in the afferent nerve terminals of the carotid body. PMID: 24906290
5. both VGluT1 and VGluT2 immunolabelings were found in ventral region of anterodorsal thalamic nucleus; many double-labeled axon terminals were also found in rostral part of the reticular thalamic nucleus and layers Ia and III-IV of the retrosplenial granular b cortex; some were also found in layer Ia of the retrosplenial granular a cortex PMID: 24639017
6. The results indicate that glutamatergic Vsp neurons sending their axons to the thalamic regions express VGLUT2. PMID: 23380804
7. This study demonistrated that Anterograde tracing from the vagus was combined with immunohistochemistry for VGLUT1 or VGLUT2 in medial nucleus tractus solitarii in the rat. PMID: 23897509
8. These results provide evidence for the involvement of VGLUT2 in the modulation of basal ganglia cicuits PMID: 24308494
9. Natural rewards and pain have distinct effects on the VGLUT expression pattern in the nucleus accumbens. PMID: 23835161
10. This study demonistrated that the estrogen receptor alpha protein in glutamatergic (vesicular glutamate transporter 2 immunoreactive) neurons of the female rat hypothalamus and amygdala. PMID: 23543101
11. VGLUT2-positive terminals make up 39.4% of excitatory thalamostriatal terminals. PMID: 23047588
12. BDNF stimulation upregulates the number, integrated density and intensity of VGLUT1 and VGLUT2 puncta in neurites of cultured hippocampal neurons (DIV7 PMID: 23326507
13. Amplification of VGLUT-2 expression after acoustic overexposure could be a compensatory mechanism from vestibular inputs in response to hearing loss and to a decrease of VGLUT-1 expression from auditory nerve fibers. PMID: 22570693
14. VGluT1 and VGluT2 are expressed in the periodontal Ruffini endings of the rat incisor. PMID: 21957077
15. Expressions of VGLUT2 mRNA are found in the olfactory epithelium, migratory cells and telencephalon during embryonic development. PMID: 21609737
16. The nocturnal elevation in VGLUT2 mRNA level in the pineal gland diminished under the constant light condition but persisted under the constant dark condition. PMID: 21982845
17. Data show that dopaminergic axons and terminals in the dorsolateral striatum and ventral striatum do not express VGluT1, VGluT2 or VGluT3, and suggest that in the normal, adult rat striatum, dopaminergic axons do not co-release glutamate. PMID: 21375596
18. These results suggest that most corneal afferents contain a neuropeptide or VGluT1/2, but rarely both. PMID: 20593358
19. trigeminal nucleus neurons expressing VGLUT1 and VGLUT2 project to the posteromedial ventral nucleus, but not to the posterior nuclei PMID: 20533365
20. The presence and co-localization of vesicular glutamate transporter 2 and PACAP in retinal ganglion cell and their projections in the brain, is described. PMID: 20339872
21. The phrenic nucleus (PhN) contained large numbers of VGLUT2-ir axon terminals, some of which made axosomatic and axodendritic synapses with PhN motoneurons. PMID: 20217347
22. The presence of two different subtypes of Vgluts, the size differences of the Vglut synaptic boutons, and their preference for different postsynaptic targets suggest that the action of glutamate on BF neurons may arise from multiple afferent sources. PMID: 19778580
23. results suggest a regression of the VGLUT2 phenotype of dopamine neurons with age, following normal development, lesion, or sprouting after injury, and a role for glutamate in the establishment of synapses by these neurons PMID: 19844994
24. Trigeminal ganglion neurons express VGluT2 more frequently than VGluT1; VGluT2 is coexpressed with VGluT1 in the cell bodies and axon terminals in most trigeminal ganglion neurons. PMID: 12815758
25. In the nucleus tractus solitarii there is a high density of VGluT2-immunoreactive fibers in the gelatinosus subnucleus and subpostremal area, and a moderate density in dorsolateral and medial subnuclei and central and lateral subnuclei. PMID: 14667459
26. The vesicular glutamate transporter 2 (VGLUT2)is a transmembrane protein responsible for loading glutamate into synaptic vesicles. PMID: 14677070
27. VGLUT1 and VGLUT2 transcripts are colocalized in most sensory neurons of the dorsal root ganglia, as well as in motor neurons of the ventral horn. PMID: 14681932
28. We found the majority of isolated dopamine neurons express VGLUT2, but not VGLUT1 or 3; our results provide a basis for the ability of dopamine neurons to release glutamate as a cotransmitter PMID: 15009640
29. data indicate that VGLUTs play a functional role in exocytotic glutamate release from astrocytes PMID: 15028755
30. VGLUT2 immunoreactivity was widespread in all spinal cord laminae, with higher intensities in LII and lateral LV, complementing VGLUT1 distribution. VGLUT2 immunoreactivity did not change after rhizotomy. PMID: 15065123
31. the major termination fields for GnRH-secreting axons, demonstrated the frequent occurrence of VGLUT2 immunoreactivity in GnRH axon terminals PMID: 15205380
32. A large population of VGLUT2-immunoreactive neurons is located primarily in the posterior division of the septum. PMID: 15224985
33. Presence of intense VGLUT2 immunoreactivity in neurons actively migrating from the olfactory placode suggests that this transporter is involved in the migratory process of these neurons. PMID: 15305861
34. VGLUT1 and VGLUT2, two subtypes of vesicular glutamate transporters, are expressed in rat spinal motoneurons. PMID: 15379996
35. the most likely sources of VGLUT2-positive boutons in the dentate supragranular layer, the CA2 area, as well as in the stratum lacunosum-moleculare of the CA1 field, might be the mossy cells PMID: 15382259
36. Vglut2 afferents to the medial prefrontal and primary somatosensory cortices. PMID: 15682395
37. The developmental pattern of Vglut2 in the olivary nucleus was studied. PMID: 15714284
38. Analysis of vGlut1 distribution in rat dorsal raphe nucleus reveals different populations of glutamate-containing axons innervating selective dendritic domains of serotonergic and non-serotonergic neurons suggesting they play different functional roles. PMID: 15845085
39. VGLUT1 and VGLUT2 proteins may often transport glutamate into vesicles within the same neuron, especially during early postnatal development, and that they are expressed widely in presumed glutamatergic, GABAergic, and cholinergic neurons, and astrocytes PMID: 15983996
40. Intracortical VGLUT1/VGLUT2 coexpressing neurons can independently modulate level of expression of either transporter at discrete synapses. May serve as plastic interface between subcortical thalamic input (VGLUT2) and cortical output (VGLUT1) neurons. PMID: 16079394
41. mRNA expressed expressed in thalamic neurons in mapping brain circuits. PMID: 16423326
42. The robust increase in vesicular glutamate transporter-2 mRNA and immunoreactivity after salt loading suggests that the cellular levels of vesicular glutamate transporter-2 in vasopressin neurons are regulated by alterations in water-electrolyte balance. PMID: 16481069
43. VGLUT2 was cocontained in small numbers of varicosities in laminae III-IV and IX. Anterogradely labeled spinocervical tract terminals in the lateral cervical nucleus were VGLUT2 immunoreactive. PMID: 16786558
44. These results indicate that vesicular glutamate transporter (VGLUT)2 is principally expressed in vasopressin neurons and also in some oxytocin neurons and that VGLUT2 in these neurons is involved in osmotic regulation. PMID: 16842872
45. VGLUT2 appears to possess two intrinsic transport machineries that are independent of each other: a DeltaPsi-dependent l-glutamate uptake and a Na(+)-dependent P(i) uptake PMID: 17046815
46. Neurosecretory neurons in the paraventricular, periventricular, supraoptic nuclei and the preoptic area secrete glutamate into the fenestrated vessels of the median eminence and posterior pituitary. PMID: 17175111
47. In accordance with current beliefs, we found significant expression of VGLUT2 mRNA in all the thalamic nuclei, while moderate expression of VGLUT1 mRNA was consistently found in both the principal relay and the association thalamic nuclei. PMID: 17299752
48. significant decline in the numbers of VGLUT2 immunoreactive boutons on the more distal dendrites of motoneurons in aged rats PMID: 17337147
49. Profiles containing both CRF- and either vGlut1- or vGlut2-immunoreactivity were apparent in the dorsal raphe nucleus PMID: 17825268
50. VGLUT2 is the only vesicular glutamate transporter expressed in thalamostriatal-projecting neurons located in the midline and intralaminar nuclei, whereas all neurons from the ventral thalamic nuclei innervating the striatum express both VGLUTs. PMID: 17826944
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亞細胞定位：

Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane; Multi-pass membrane protein. Cell junction, synapse, synaptosome.
蛋白家族：

Major facilitator superfamily, Sodium/anion cotransporter family, VGLUT subfamily
組織特異性：

Expressed in brain (at protein level). Expressed in brainstem, deep nuclei, septal nuclei, nuclei of the diagonal band, frontal cortex, hypothalamus, midbrain, parietal cortex and temporal cortex. Also expressed in pineal gland and islets of Langerhans.
數據庫鏈接：

KEGG: rno:84487

STRING: 10116.ENSRNOP00000022383

UniGene: Rn.19372