Recombinant Rat Trifunctional enzyme subunit alpha, mitochondrial (Hadha)

中文名稱：

大鼠Hadha重組蛋白
貨號：

CSB-YP731053RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Hadha重組蛋白
貨號：

CSB-EP731053RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Hadha重組蛋白
貨號：

CSB-EP731053RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Hadha重組蛋白
貨號：

CSB-BP731053RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Hadha重組蛋白
貨號：

CSB-MP731053RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Hadha
Uniprot No.：

Q64428
別名：

HadhaTrifunctional enzyme subunit alpha; mitochondrial; Monolysocardiolipin acyltransferase; EC 2.3.1.-; TP-alpha) [Includes: Long-chain enoyl-CoA hydratase; EC 4.2.1.17); Long chain 3-hydroxyacyl-CoA dehydrogenase; EC 1.1.1.211)]
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length of Mature Protein
表達區域：

37-763
氨基酸序列

SRTH INYGVKGDVA VIRINSPNSK VNTLNKEVQS EFVEVMNEIW ANDQIRSAVL ISSKPGCFVA GADINMLASC TTPQEAARIS QEGQKMFEKL EKSPKPVVAA ISGSCLGGGL ELAIACQYRI ATKDRKTVLG VPEVLLGILP GAGGTQRLPK MVGVPAAFDM MLTGRNIRAD RAKKMGLVDQ LVDPLGPGIK SPEERTIEYL EEVAVNFAKG LADRKVSAKQ SKGLMEKLTS YAMTIPFVRQ QVYKTVEEKV KKQTKGLYPA PLKIIDAVKT GLEQGNDAGY LAESEKFGEL ALTKESKALM GLYNGQVLCK KNKFGAPQKT VQQLAILGAG LMGAGIAQVS VDKGLKTLLK DTTVTGLGRG QQQVFKGLND KVKKKALTSF ERDSIFSNLI GQLDYKGFEK ADMVIEAVFE DLAVKHKVLK EVESVTPEHC IFASNTSALP INQIAAVSQR PEKVIGMHYF SPVDKMQLLE IITTDKTSKD TTASAVAVGL KQGKVIIVVK DGPGFYTTRC LAPMMSEVIR ILQEGVDPKK LDALTTGFGF PVGAATLADE VGIDVAQHVA EDLGKAFGER FGGGSVELLK LMVSKGFLGR KSGKGFYIYQ SGSKNKNLNS EIDNILVNLR LPAKPEVSSD EDIQYRVITR FVNEAVLCLQ EGILATPEEG DIGAVFGLGF PPCLGGPFRF VDLYGAQKVV DRLRKYESAY GTQFTPCQLL RDLANNSSKK FYQ
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Mitochondrial trifunctional enzyme catalyzes the last three of the four reactions of the mitochondrial beta-oxidation pathway. The mitochondrial beta-oxidation pathway is the major energy-producing process in tissues and is performed through four consecutive reactions breaking down fatty acids into acetyl-CoA. Among the enzymes involved in this pathway, the trifunctional enzyme exhibits specificity for long-chain fatty acids. Mitochondrial trifunctional enzyme is a heterotetrameric complex composed of two proteins, the trifunctional enzyme subunit alpha/HADHA described here carries the 2,3-enoyl-CoA hydratase and the 3-hydroxyacyl-CoA dehydrogenase activities while the trifunctional enzyme subunit beta/HADHB bears the 3-ketoacyl-CoA thiolase activity. Independently of the subunit beta, the trifunctional enzyme subunit alpha/HADHA also has a monolysocardiolipin acyltransferase activity. It acylates monolysocardiolipin into cardiolipin, a major mitochondrial membrane phospholipid which plays a key role in apoptosis and supports mitochondrial respiratory chain complexes in the generation of ATP. Allows the acylation of monolysocardiolipin with different acyl-CoA substrates including oleoyl-CoA for which it displays the highest activity.
基因功能參考文獻：
1. The catalytic residues of the MTP beta-subunit are positioned in the active site similarly to those of monofunctional 3-ketoacyl-CoA thiolase. PMID: 18640292
亞細胞定位：

Mitochondrion. Mitochondrion inner membrane.
蛋白家族：

Enoyl-CoA hydratase/isomerase family; 3-hydroxyacyl-CoA dehydrogenase family
數據庫鏈接：

KEGG: rno:170670

STRING: 10116.ENSRNOP00000038073

UniGene: Rn.3340