Recombinant Rat Transitional endoplasmic reticulum ATPase (Vcp), partial

中文名稱：

大鼠Vcp重組蛋白
貨號：

CSB-YP025813RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Vcp重組蛋白
貨號：

CSB-EP025813RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Vcp重組蛋白
貨號：

CSB-EP025813RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Vcp重組蛋白
貨號：

CSB-BP025813RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Vcp重組蛋白
貨號：

CSB-MP025813RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Vcp
Uniprot No.：

P46462
別名：

Vcp; Transitional endoplasmic reticulum ATPase; TER ATPase; EC 3.6.4.6; 15S Mg(2+)-ATPase p97 subunit; Valosin-containing protein; VCP
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Necessary for the fragmentation of Golgi stacks during mitosis and for their reassembly after mitosis. Involved in the formation of the transitional endoplasmic reticulum (tER). The transfer of membranes from the endoplasmic reticulum to the Golgi apparatus occurs via 50-70 nm transition vesicles which derive from part-rough, part-smooth transitional elements of the endoplasmic reticulum (tER). Vesicle budding from the tER is an ATP-dependent process. The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope. Regulates E3 ubiquitin-protein ligase activity of RNF19A. Component of the VCP/p97-AMFR/gp78 complex that participates in the final step of the sterol-mediated ubiquitination and endoplasmic reticulum-associated degradation (ERAD) of HMGCR. Involved in endoplasmic reticulum stress-induced pre-emptive quality control, a mechanism that selectively attenuates the translocation of newly synthesized proteins into the endoplasmic reticulum and reroutes them to the cytosol for proteasomal degradation. Plays a role in the regulation of stress granules (SGs) clearance process upon arsenite-induced response. Also involved in DNA damage response: recruited to double-strand breaks (DSBs) sites in a RNF8- and RNF168-dependent manner and promotes the recruitment of TP53BP1 at DNA damage sites. Recruited to stalled replication forks by SPRTN: may act by mediating extraction of DNA polymerase eta (POLH) to prevent excessive translesion DNA synthesis and limit the incidence of mutations induced by DNA damage. Together with SPRTN metalloprotease, involved in the repair of covalent DNA-protein cross-links (DPCs) during DNA synthesis. Involved in interstrand cross-link repair in response to replication stress by mediating unloading of the ubiquitinated CMG helicase complex. Required for cytoplasmic retrotranslocation of stressed/damaged mitochondrial outer-membrane proteins and their subsequent proteasomal degradation. Essential for the maturation of ubiquitin-containing autophagosomes and the clearance of ubiquitinated protein by autophagy. Acts as a negative regulator of type I interferon production by interacting with DDX58/RIG-I: interaction takes place when DDX58/RIG-I is ubiquitinated via 'Lys-63'-linked ubiquitin on its CARD domains, leading to recruit RNF125 and promote ubiquitination and degradation of DDX58/RIG-I. May play a role in the ubiquitin-dependent sorting of membrane proteins to lysosomes where they undergo degradation. May more particularly play a role in caveolins sorting in cells. By controlling the steady-state expression of the IGF1R receptor, indirectly regulates the insulin-like growth factor receptor signaling pathway.
基因功能參考文獻：
1. Valosin-containing protein is a novel repressor of cardiomyocyte hypertrophy induced by pressure overload. PMID: 28799247
2. VCP and Jab1 provide a novel signaling pathway for testicular ischemia and may play an important role in ischemia-reperfusion injury induced cell death in rat testis. PMID: 26754107
3. VCP may play an essential role in the initiation of autophagy. PMID: 27000202
4. conclude that the Akt substrate, VCP, mediates the increased expression of iNOS downstream from Hsp22 through an NF-kappaB-dependent mechanism PMID: 23737493
5. Investigations into expression and interaction of p97/VCP and Smad1 in postnatal testis and epididymis support roles in spermatogenesis. PMID: 22051847
6. These results suggest that an altered expression pattern of p97/VCP and ubiquitin in the developing rat retina may associate with age-related retinal degeneration. PMID: 22102169
7. Immunoprecipitation from rat brain shows that neurofibromin forms a complex with VCP and p47. The C-terminal D1D2 region is required. PMID: 22105171
8. Stalled proteasomes are directly relieved by P97 recruitment PMID: 21733848
9. p97 AAA ATPase can regulate physiological CYP3A content and thus influence its clinically relevant function PMID: 21107009
10. Valosin-containing protein (VCP) has a role in novel feedback machinery between abnormal protein accumulation and transcriptional suppression PMID: 20410307
11. 26S proteasomes and p97/VCP complexes bind to the ubiquitin-like domain of HHR23B. PMID: 19182904
12. p97/VCP is essential for degrading membrane-associated ubiquitinated proteins and that profound deficits in its ATPase activity severely affect ER quality control, leading to abnormal ER expansion and cell death PMID: 12351637
13. Dual VCP functions as an aggregate formase and an unfoldase. PMID: 17584300
14. analysis of nucleotide binding to P97 reveals the properties of a tandem AAA hexameric ATPase PMID: 18332143
15. Results show that VCP 16 sites are in the N-terminal region and 16 sites are clustered in D2alpha domain (from Pro646 to Gly765). PMID: 19335618
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亞細胞定位：

Cytoplasm, cytosol. Endoplasmic reticulum. Nucleus. Cytoplasm, Stress granule.
蛋白家族：

AAA ATPase family
數據庫鏈接：

KEGG: rno:116643

STRING: 10116.ENSRNOP00000040121

UniGene: Rn.98891