Recombinant Rat Sodium- and chloride-dependent betaine transporter (Slc6a12), partial

中文名稱：

大鼠Slc6a12重組蛋白
貨號：

CSB-YP021690RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Slc6a12重組蛋白
貨號：

CSB-EP021690RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Slc6a12重組蛋白
貨號：

CSB-EP021690RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Slc6a12重組蛋白
貨號：

CSB-BP021690RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Slc6a12重組蛋白
貨號：

CSB-MP021690RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Slc6a12
Uniprot No.：

P48056
別名：

Slc6a12; Sodium- and chloride-dependent betaine transporter; Na(+)/Cl(-) betaine/GABA transporter; Solute carrier family 6 member 12
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Transports betaine and GABA. May have a role in regulation of GABAergic transmission in the brain through the reuptake of GABA into presynaptic terminals, as well as in osmotic regulation.
基因功能參考文獻：
1. GAT-1, which is predominantly presynaptically located, is the major GABA transporter in the hippocampus under baseline, quiescent conditions. PMID: 23381899
2. we report the identification of a unique amino acid motif in the vesicular GABA transporter (VGAT) that controls its synaptic localization and activity-dependent recycling PMID: 23804087
3. The results demonstrate a malfunction of GAT-1 in thalamic astrocytes of a well-established genetic model of absence epilepsy, and indicate that this abnormality is not a reflection of a general pathology affecting all astrocytic functions. PMID: 23090943
4. This study provides evidence that the GAT-1-mediated regulation of extracellular GABA can modulate glutamatergic transmission at the subthalamopallidal synapse in the rat globus pallidus. PMID: 22616751
5. BDNF enhances the time span of GAT-1 molecules at the plasma membrane of astrocytes. PMID: 21969376
6. These experiments confirmed that, when a high concentration of GABA is present in the cytoplasm, GAT1 can operate in reverse mode. The reverse transport was affected by the the internal concentrations of Na+ and Cl-. PMID: 21775701
7. Data indicate that GAT-1 and GAT-3 represent different target sites through which GABA reuptake may subserve complementary regulation of GABAergic transmission in the rat globus pallidus. PMID: 21410779
8. The GABA transporter GAT-1 is upregulated with no change in GAT-3 or glutamate transporters. PMID: 20542084
9. During neocortical development the expression of GABA transporter in the cerebral cortex slightly precedes the complete maturation of inhibitory synaptogenesis and suggests that it may contribute to the formation of neocortical GABAergic circuitry. PMID: 12614674
10. GAT1 mRNA expression is initially up-regulated in the ipsilateral vestibular nucleus and then undergoes a bilateral increase 50 h after labyrinthectomy. PMID: 14663191
11. GABA transporter can exist in either of two mutually exclusive phosphorylation states and that the relative abundance of these states determines in part the relative subcellular distribution of the transporter PMID: 14761965
12. two discontinuous segments in the carboxyl terminus are required for membrane targeting of the rat GAT1 PMID: 15073174
13. GAT-1 modulates synaptic transmission under physiological conditions in the early postnatal rat hippocampus. PMID: 15229234
14. GAT-1 immunohistochemistry is the most robust means of visualising chandelier cell cartridges in rat medial prefrontal cortex and hippocampus. PMID: 15649449
15. The data, show that GAT-1 contributes to the termination of the synaptic action of mossy fiber-released GABA and rules out its involvement in depolarization-dependent GABA release. PMID: 15668946
16. study of an acutely recycling pool of endogenous gamma-aminobutyric acid transporters (GAT1) in cortical neurons that comprises approximately one-third of total cellular GAT1 PMID: 15778221
17. Following transient focal ischemia, mRNA expression of the vesicular GABA transporter (VGAT) decreased significantly by 3 h of reperfusion and remained at a significantly lower level than sham until at least 72 h of reperfusion. PMID: 15936116
18. glutamine 291 residue is involved in the interaction with sodium or in maintaining the cation accessibility to the transporter(GAT-1) PMID: 16378241
19. The steady expression of vesicular GABA transporter mRNA and protein after photothrombosis indicates that the exocytotic release mechanism of GABA is not affected by ischemia. PMID: 16616431
20. SNAP-25 binding to syntaxin 1A greatly potentiates the physical interaction of syntaxin 1A with GAT-1 and significantly enhances the syntaxin 1A-mediated inhibition of GAT-1 reuptake function. PMID: 16861228
21. significant role for GAT-1 in the control of the excitability of immature hippocampal neurons and networks. PMID: 17298599
22. We examined GAT-1, a marker of chandelier cartridges. We show that the numbers of arrays of chandelier axons are reduced by 36% in the ventral prelimbic cortex of isolation-reared rats, compared with their socially-housed litter mates. PMID: 18512213
23. GAT-1 mRNA (a prevalent presynaptic component) correlates with avoidances and escapes in rat cerebellum, while glial fibrillary acid protein mRNA (an astrocytic component) correlates with freezings in cerebellum and cerebral cortex. PMID: 19235900
24. Results indicate that calpain activation/inhibition in GABAergic terminals may influence the scaffolding and surface expression of GABA transporter GAT1 under normal conditions or imbalance GAT1-mediated GABAergic transmission under pathological states PMID: 19576516
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亞細胞定位：

Membrane; Multi-pass membrane protein.
蛋白家族：

Sodium:neurotransmitter symporter (SNF) (TC 2.A.22) family, SLC6A12 subfamily
數據庫鏈接：

KEGG: rno:50676

STRING: 10116.ENSRNOP00000018821

UniGene: Rn.11352