Recombinant Rat Pyruvate kinase isozymes R/L (Pklr)

中文名稱：

大鼠Pklr重組蛋白
品名簡稱：

Recombinant Rat Pklr protein
貨號：

CSB-EP018071RA
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Pklr
Uniprot No.：

P12928
別名：

PklrPyruvate kinase PKLR; EC 2.7.1.40; L-PK; Pyruvate kinase isozymes L/R
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Full Length
來源：

E.coli
分子量：

66.2kDa
表達區域：

1-574aa
氨基酸序列

MSVQENTLPQQLWPWIFRSQKDLAKSALSGAPGGPAGYLRRASVAQLTQELGTAFFQQQQLPAAMADTFLEHLCLLDIDSQPVAARSTSIIATIGPASRSVDRLKEMIKAGMNIARLNFSHGSHEYHAESIANIREATESFATSPLSYRPVAIALDTKGPEIRTGVLQGGPESEVEIVKGSQVLVTVDPKFQTRGDAKTVWVDYHNITRVVAVGGRIYIDDGLISLVVQKIGPEGLVTEVEHGGILGSRKGVNLPNTEVDLPGLSEQDLLDLRFGVQHNVDIIFASFVRKASDVLAVRDALGPEGQNIKIISKIENHEGVKKFDEILEVSDGIMVARGDLGIEIPAEKVFLAQKMMIGRCNLAGKPVVCATQMLESMITKARPTRAETSDVANAVLDGADCIMLSGETAKGSFPVEAVMMQHAIAREAEAAVYHRQLFEELRRAAPLSRDPTEVTAIGAVEASFKCCAAAIIVLTKTGRSAQLLSQYRPRAAVIAVTRSAQAARQVHLSRGVFPLLYREPPEAIWADDVDRRVQFGIESGKLRGFLRVGDLVIVVTGWRPGSGYTNIMRVLSVS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Plays a key role in glycolysis.
基因功能參考文獻：
1. Studied the effect of the point mutations around this phosphorylation site on the catalytic properties of L-pyruvate kinase. PMID: 22878931
2. c-Myc is required for the glucose-mediated induction of this metabolic enzyme. PMID: 12480946
3. We observed that cystine inhibited the enzyme pyruvate kinase activity non-competitively in a dose- and time-dependent way. PMID: 15196592
4. cooperative interaction between NF1 family members and HNF1alpha plays an important role in hepatic L-PK transcription PMID: 16204235
5. Regulation of hepatic L-pyruvate kinase promoter composition and activity PMID: 16644726
6. A novel chimeric promoter, consisting of a 1.6 kb glucose 6-phosphatase promoter and a segment of the regulatory element derived from the L-type pyruvate kinase promoter, was designed to provide strong and tight control of insulin expression in liver. PMID: 17160355
7. Maximal glucose-induced expression of the L-PK gene in INS-1-derived 832/13 cells involves increased c-Myc abundance, recruitment of c-Myc, Max, and ChREBP to the promoter, and a glucose-stimulated increase in ChREBP transactivation. PMID: 17341548
8. A detailed analysis of the molecular events of the insulin-independent glucose response of the liver-type pyruvate kinase (L-PK) gene was performed. PMID: 18468514
9. cAMP and glucose signaling converge on a complex containing ChREBP, HNF4alpha, and CBP, and cAMP acts by disrupting this transcriptional complex assembled by glucose-derived signals PMID: 19406844
10. Data show that glucose regulates acetylation and methylation of various histone residues at the L-PK gene promoter. PMID: 19631660
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蛋白家族：

Pyruvate kinase family
數據庫鏈接：

KEGG: rno:24651

STRING: 10116.ENSRNOP00000027700

UniGene: Rn.48821