Recombinant Rat Potassium voltage-gated channel subfamily B member 1 (Kcnb1), partial

中文名稱：

大鼠Kcnb1重組蛋白
貨號：

CSB-YP012016RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Kcnb1重組蛋白
貨號：

CSB-EP012016RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Kcnb1重組蛋白
貨號：

CSB-EP012016RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Kcnb1重組蛋白
貨號：

CSB-BP012016RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Kcnb1重組蛋白
貨號：

CSB-MP012016RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Kcnb1
Uniprot No.：

P15387
別名：

Kcnb1; Potassium voltage-gated channel subfamily B member 1; Delayed rectifier potassium channel 1; DRK1; Voltage-gated potassium channel subunit Kv2.1
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Voltage-gated potassium channel that mediates transmembrane potassium transport in excitable membranes, primarily in the brain, but also in the pancreas and cardiovascular system. Contributes to the regulation of the action potential (AP) repolarization, duration and frequency of repetitive AP firing in neurons, muscle cells and endocrine cells and plays a role in homeostatic attenuation of electrical excitability throughout the brain. Plays also a role in the regulation of exocytosis independently of its electrical function. Forms tetrameric potassium-selective channels through which potassium ions pass in accordance with their electrochemical gradient. The channel alternates between opened and closed conformations in response to the voltage difference across the membrane. Homotetrameric channels mediate a delayed-rectifier voltage-dependent outward potassium current that display rapid activation and slow inactivation in response to membrane depolarization. Can form functional homotetrameric and heterotetrameric channels that contain variable proportions of KCNB2; channel properties depend on the type of alpha subunits that are part of the channel. Can also form functional heterotetrameric channels with other alpha subunits that are non-conducting when expressed alone, such as KCNF1, KCNG1, KCNG3, KCNG4, KCNH1, KCNH2, KCNS1, KCNS2, KCNS3 and KCNV1, creating a functionally diverse range of channel complexes. Heterotetrameric channel activity formed with KCNS3 show increased current amplitude with the threshold for action potential activation shifted towards more negative values in hypoxic-treated pulmonary artery smooth muscle cells. Channel properties are also modulated by cytoplasmic ancillary beta subunits such as AMIGO1, KCNE1, KCNE2 and KCNE3, slowing activation and inactivation rate of the delayed rectifier potassium channels. In vivo, membranes probably contain a mixture of heteromeric potassium channel complexes, making it difficult to assign currents observed in intact tissues to any particular potassium channel family member. Major contributor to the slowly inactivating delayed-rectifier voltage-gated potassium current in neurons of the central nervous system, sympathetic ganglion neurons, neuroendocrine cells, pancreatic beta cells, cardiomyocytes and smooth muscle cells. Mediates the major part of the somatodendritic delayed-rectifier potassium current in hippocampal and cortical pyramidal neurons and sympathetic superior cervical ganglion (CGC) neurons that acts to slow down periods of firing, especially during high frequency stimulation. Plays a role in the induction of long-term potentiation (LTP) of neuron excitability in the CA3 layer of the hippocampus. Contributes to the regulation of glucose-induced action potential amplitude and duration in pancreatic beta cells, hence limiting calcium influx and insulin secretion. Plays a role in the regulation of resting membrane potential and contraction in hypoxia-treated pulmonary artery smooth muscle cells. May contribute to the regulation of the duration of both the action potential of cardiomyocytes and the heart ventricular repolarization QT interval. Contributes to the pronounced pro-apoptotic potassium current surge during neuronal apoptotic cell death in response to oxidative injury. May confer neuroprotection in response to hypoxia/ischemic insults by suppressing pyramidal neurons hyperexcitability in hippocampal and cortical regions. Promotes trafficking of KCNG3, KCNH1 and KCNH2 to the cell surface membrane, presumably by forming heterotetrameric channels with these subunits. Plays a role in the calcium-dependent recruitment and release of fusion-competent vesicles from the soma of neurons, neuroendocrine and glucose-induced pancreatic beta cells by binding key components of the fusion machinery in a pore-independent manner.
基因功能參考文獻：
1. up-regulation of the KV2.1 channel in renal artery myocytes may be involved in the compensatory mechanisms against decreased renal blood flow in salt-sensitive hypertension PMID: 28867730
2. Trafficking of the Kv2.1 channel takes place in the somatodendritic plasma membrane and in the axon initial segment. PMID: 29042434
3. Data suggest that NMDAR plays key role in mediating effect of leptin to modulate function of insulin-secreting cells by promoting AMPK-dependent trafficking of KATP and Kv2.1 channels to plasma membrane. (NMDAR = N-methyl-D-aspartate receptor; AMPK = AMP-activated protein kinase; KATP = ATP-sensitive potassium channel; Kv2.1 = delayed-rectifier potassium channel 1) PMID: 28768770
4. The experiments presented here demonstrate that the cell death-specific role of Kv2.1 can be uniquely modulated to provide neuroprotection in an animal model of acute ischemic stroke. We thus reveal a novel therapeutic strategy for neurological disorders that are accompanied by Kv2.1-facilitated forms of cell death. PMID: 28483976
5. Taken together, these results suggest that HIV-1 gp120 induces hippocampal neuron apoptosis by enhancement of the Ik, which might be associated with increased Kv2.1 expression via the p38 MAPK pathway. PMID: 26393286
6. Structural evidence shows that two distinct domains form the C-terminal part within the Kv2.1 ion channel. PMID: 25256718
7. modifications in Kv2.1 are dependent on the activation of the chemokine co-receptors CCR5 and CXCR4 PMID: 24086760
8. From histological assessment illustrates that the Kv2.1 and Kv.2 subunits are widely expressed in a mixture of DRG neurons and appear enriched in the myelinated neuron population. PMID: 24252178
9. Convergent Ca2+ and Zn2+ signaling regulates apoptotic Kv2.1 K+ currents. PMID: 23918396
10. distinct modifications in Kv2.1 in response to short- and long-term CXCR4-mediated signaling could provide a basis for neuroprotection or apoptosis in neuropathologies. PMID: 23223293
11. The present data provide evidence that the transcriptional regulation of Kcnv2 and Kv2.1 is a way through which the retinal clock system drives the functional adaptation of visual function to the marked daily changes in environmental lighting conditions. PMID: 22969075
12. the molecular determinants of Kv2.1 localization and modulation are distinct regions of the channel that function independently. PMID: 22554782
13. Direct interaction between syntaxin 1A and the Kv2.1 C-terminus is required for efficient insulin exocytosis and glucose-stimulated insulin secretion. PMID: 22411134
14. The elevation of membrane cholesterol enhances susceptibility of cerebellar granule neurons to apoptotic stimuli via cyclic AMP/PKA/CREB-dependent up-regulation of Kv2.1. PMID: 22118516
15. TGF-beta1 upregulates Kv2.1 channel expression and I(K) currents and thereby promotes cerebellar granule neuron maturation PMID: 21412780
16. specific residues within the S3-S4 and S5-P-loop linkers may play important roles in Kv2.1 U-type inactivation PMID: 21806933
17. Immunocytochemistry indicated that protein expression for Kv2.1 increased between 1 wk and 4-5 wk of age. PMID: 21451062
18. In middle cerebral arteries control of arterial diameter is associated with heteromultimeric Kv2.1/Kv9.3 expression. PMID: 20876197
19. Kv2.1 channel expression at the mRNA and protein levels was greatly up-regulated in pulmonary arterial smooth muscle cells and pulmonary artery under hypoxia after blockade of endogenous 15-hydroxyeicosatetraenoic acid production. PMID: 20680544
20. analysis of binding sites of structurally different antiarrhythmics flecainide and propafenone in the subunit interface of potassium channel Kv2.1 PMID: 20709754
21. regulates exocytosis in neuroendocrine and nerve cells PMID: 20484665
22. These results suggest that the cellular metabolism including MgATP production and/or channel phosphorylation/dephosphorylation underlie the physiological modulation of Kv2.1 channels during glucose-induced insulin secretion. PMID: 20403337
23. A rise in neuronal free Zn(2+) is critical in altering Kv2.1 channel activity and localization following ischemia. The results may lead to a better understanding of cellular adaptive responses to injury. PMID: 20092568
24. exposed residues in the T1 domain of the N terminus, as well as the CTA domain in the C terminus, are important in determining channel activation kinetics and that these N- and C-terminal regions interact PMID: 12560340
25. In pancreatic beta-cells, Kv2.1 channels mediate a fast-inactivating K(+) current at physiological temperatures and may be regulated by the metabolic generation of NADPH. PMID: 12562993
26. Tyr(124) is a significant site at which the mutually antagonistic activities of Src and protein-tyrosine phosphatase epsilon affect Kv2.1 phosphorylation and activity PMID: 12615930
27. different Kv9.3/Kv2.1 ratios could have physiological implications in lung and brain during post-natal development, and that diet composition and selective tissue-specific insulin regulation modulate the expression of Kv2.1 and Kv9.3. PMID: 12744302
28. Kv2.1-encoded K+ channels are necessary for the apoptotic signaling cascade in mammalian cortical neurons in culture and are sufficient for increasing the susceptibility to apoptogens in a nonexcitable cell PMID: 12832499
29. the delayed rectifier potassium current is modulated by angiotensin II in rat cells PMID: 14550259
30. Strategic localization of Kv2.1 subunit-containing channels at specific postsynaptic sites suggests that this family of voltage-activated K+ channels may have additional roles and/or regulatory components. PMID: 14608003
31. Kv2.1 channel gating and insulin exocytosis are modified by disruption of pancreatic beta-cell lipid rafts PMID: 15073181
32. NFATc3 controls the excitability of arterial smooth muscle by regulating Kv2.1 expression PMID: 15322114
33. Targetted transfer of rat Kv2.1 to preterm rabbit ductus arteriosus smooth muscle tissue restores O2-responsive constriction & potassium currens. PMID: 15353504
34. A biochemical characterization of Kv2.1 channel complexes from both recombinant cell lines and native rat brain. PMID: 16008572
35. Kv2.1 can act as novel Ca2+- and metabolic state-sensitive K+ channel, and dynamic modulation of IK/Kv2.1 in response to hypoxia/ischemia suppresses neuronal excitability and could confer neuroprotection in response to brief ischemic insults. PMID: 16319318
36. Kv2.1 cytoplasmic C-terminal domain can act as an autonomous domain sufficient to transfer Kv2.1-like clustering, voltage-dependent activation, and cholinergic modulation to diverse Kv channels. PMID: 16407566
37. We conclude that different domains within SNAP-25 can form distinct complexes with Kv2.1 to execute a fine allosteric regulation of channel gating and the architecture of the outer pore structure in order to modulate cell excitability. PMID: 16478442
38. results show that variable phosphorylation of Kv2.1 at a large number of sites allows graded activity-dependent regulation of channel gating and neuronal firing properties PMID: 16917065
39. Kv2.1-containing microdomains on the surface membrane of human HEK cells and cultured hippocampal neurons are stable, yet dynamic, structures on the cell surface that are specialized sites for the concentration of Kv2.1. PMID: 16988031
40. Kv2.1 current in neocortical pyramidal cells activated and inactivated at relatively negative potentials and was very sensitive to holding potential. PMID: 17379638
41. early expression of delayed rectifier and A-type K(+) currents and channels associated with Kv 2.1 and Kv 4.3 expression in embryonic neural progenitor cells occurs prior to cell differentiation PMID: 18270591
42. Novel insights into intrinsic mechanisms for the regulation of Kv2.1 trafficking, gating, and phosphorylation-dependent modulation through cytoplasmic N/C-terminal interaction, which resembles alpha/beta subunit interaction in other Kv channels. PMID: 18463252
43. Kv2.1 may contribute to the activity dependence of dense-core vesicles release PMID: 18542995
44. Kv2.1 clusters are strategically situated at neuroglial junctions to achieve the rapid modulation after ischemic stress via glutamate signaling. PMID: 18716211
45. the mRNA level of Kv2.1 decreased significantly in rat bladder with detrusor hyperreflexia. PMID: 18982871
46. Kv2.1 is associated with the axon initial segment both in vitro and in vivo where it may modulate action potential frequency and back propagation. It is likely multiple mechanisms regulate Kv2.1 trafficking to the cell surface. PMID: 19014551
47. Mutation of histidine 105 in the T1 domain of the potassium channel Kv2.1 disrupts heteromerization with Kv6.3 and Kv6.4. PMID: 19074135
48. NMDA receptor-dependent Kv2.1 membrane translocation is regulated by a soluble N-ethylmaleimide-sensitive factor attachment protein receptor-dependent vesicular trafficking mechanism and causes neuronal cell death induced by chronic loss of K(+). PMID: 19077057
49. Regulation of intrinsic excitability in hippocampal neurons by activity-dependent modulation of the KV2.1 potassium channel. PMID: 19276663
50. alterations of Kv1.1 and Kv2.1 might contribute to glutamate-induced toxicity in hippocampal neurons PMID: 19472219
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亞細胞定位：

Cell membrane. Perikaryon. Cell projection, dendrite. Cell projection, axon. Cell junction, synapse, postsynaptic cell membrane. Cell junction, synapse. Cell junction, synapse, synaptosome. Membrane; Multi-pass membrane protein. Lateral cell membrane. Cell membrane, sarcolemma.
蛋白家族：

Potassium channel family, B (Shab) (TC 1.A.1.2) subfamily, Kv2.1/KCNB1 sub-subfamily
組織特異性：

Expressed in brain. Expressed in the hippocampus, cerebral cortex, cerebellum, thalamus, hypothalamus, olfactory bulb, corpus striatum and medial hebenula. Expressed in pancreatic islets. Expressed in heart and skeletal muscle. Levels remain constant thro
數據庫鏈接：

KEGG: rno:25736

STRING: 10116.ENSRNOP00000065961

UniGene: Rn.26724