Recombinant Rat Plasma membrane calcium-transporting ATPase 2 (Atp2b2), partial

中文名稱：

Recombinant Rat Plasma membrane calcium-transporting ATPase 2(Atp2b2) ,partial
貨號：

CSB-YP002336RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Rat Plasma membrane calcium-transporting ATPase 2(Atp2b2) ,partial
貨號：

CSB-EP002336RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Rat Plasma membrane calcium-transporting ATPase 2(Atp2b2) ,partial
貨號：

CSB-EP002336RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Rat Plasma membrane calcium-transporting ATPase 2(Atp2b2) ,partial
貨號：

CSB-BP002336RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Rat Plasma membrane calcium-transporting ATPase 2(Atp2b2) ,partial
貨號：

CSB-MP002336RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Atp2b2
Uniprot No.：

P11506
別名：

Atp2b2Plasma membrane calcium-transporting ATPase 2; PMCA2; EC 7.2.2.10; Plasma membrane calcium ATPase isoform 2; Plasma membrane calcium pump isoform 2
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

ATP-driven Ca(2+) ion pump involved in the maintenance of basal intracellular Ca(2+) levels in specialized cells of cerebellar circuit and vestibular and cochlear systems. Uses ATP as an energy source to transport cytosolic Ca(2+) ions across the plasma membrane to the extracellular compartment. Has fast activation and Ca(2+) clearance rate suited to control fast neuronal Ca(2+) dynamics. At parallel fiber to Purkinje neuron synapse, mediates presynaptic Ca(2+) efflux in response to climbing fiber-induced Ca(2+) rise. Provides for fast return of Ca(2+) concentrations back to their resting levels, ultimately contributing to long-term depression induction and motor learning. Plays an essential role in hearing and balance. In cochlear hair cells, shuttles Ca(2+) ions from stereocilia to the endolymph and dissipates Ca(2+) transients generated by the opening of the mechanoelectrical transduction channels. Regulates Ca(2+) levels in the vestibular system, where it contributes to the formation of otoconia. In non-excitable cells, regulates Ca(2+) signaling through spatial control of Ca(2+) ions extrusion and dissipation of Ca(2+) transients generated by store-operated channels. In lactating mammary gland, allows for the high content of Ca(2+) ions in the milk.
基因功能參考文獻：
1. higher occupancy of Calm2 and Calm3 promoters by NFATc2 and increased expression of these genes in response to NFATc2 silencing were demonstrated in PMCA2 and PMCA3-reduced lines. PMID: 28153703
2. The results with PMCA2- or PMCA3-downregulated lines provide an evidence of a novel role of PMCA isoforms in regulation of bioenergetic pathways. PMID: 25276815
3. Plasma membrane PMCA2 and PMCA3 regulate cellular pH homeostasis in differentiating adrenal chromaffin cell line in a manner dependent on cytosolic Ca2+ elevations. PMID: 25014339
4. GlyT2 activity is pre-synaptically controlled by physical and functional association with plasma membrane PMCA2, PMCA3, and NCX1. PMID: 25315779
5. Results demonstrate a significant impairment of cell survival under conditions of PMCA2 suppression. Overexpression of PMCA2 rendered the neurons significantly resistant to complex I inhibition. PMID: 24269647
6. The distribution of PMCA2 at different cochlear locations during development was quantified. PMID: 22672315
7. Results suggest that PMCA2 and PMCA3 function is rather related to regulation of GABA synthesis and degradation than supplying cells with metabolites, which can be potentially energetic source. PMID: 21798237
8. Spiral ganglion cells from newborn rats strongly expresses PMCA2. PMID: 20398509
9. Data link the reduction in PMCA2 expression with perturbations in CRMP1 expression and death of spinal cord neurons, representing an additional mechanism underlying AMPA/kainate receptor-mediated excitotoxicity with relevance to neurodegeneration in EAE. PMID: 20489728
10. A novel function for PMCAs as modulators of Ca(2+) signaling at the synapse is suggested. PMID: 20678993
11. PMCA2w/a is hair-bundle isoform of PMCA in rat hair cells, and splice variant 2w targets PMCA2 to bundles. The 2w sequence is first targeting signal identified for a hair-bundle membrane protein PMID: 16763025
12. PMCA2 molecules maintain transient interactions with other components of stereocilia, and mobile pool of PMCA2 mediates exchange between stereocilia and removal and delivery sites at periphery of apical cell surface. PMID: 16763047
13. A Leu-Ile motif in 'b'-tail splice variants promotes PMCA1b and PMCA2b basolateral sorting in hair cells. PMID: 16803870
14. fast PMCA2a-mediated clearance of [Ca2+]i from presynaptic excitatory terminals regulated excitatory synaptic transmission within hippocampal CA3 PMID: 17170045
15. PMCA2 is targeted to distinct compartments within the plasma membrane. In the molecular layer, this isoform was at highest levels within postsynaptic profiles of the cerebellar cortex. PMID: 17183553
16. The evidence suggests that PMCA contributes to re-establish resting Ca(2+) levels in the cilia following olfactory responses. PMID: 17970729
17. overexpression of PMCA2 leads, in insulin-producing cells, to an imbalance between ATP generation and consumption. PMID: 18643776
18. Results suggest that the fast Ca(2+) activation kinetics and high V(max) of PMCA2a make this pump especially suited for rapid clearance of presynaptic Ca(2+) in fast-spiking inhibitory nerve terminals, which face severe transient calcium loads. PMID: 19025983
19. Results indicate that hormones affected Ca(2+) transport activity via PMCA2, and that this effect depended on both PMCA isoform composition and steroid structure. PMID: 19120150
20. Reduced expression of PMCA2a during homeostatic plasticity provides a mechanism to remodel pre-synaptic Ca2+ dynamics as a flexible way to alter glutamate release. PMID: 19410650
21. Changes in islet plasma membrane calcium ATPase activity and Atp2b2 protein isoform expression induced by insulin reistance are reported. PMID: 19653992
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亞細胞定位：

Cell membrane; Multi-pass membrane protein. Cell junction, synapse. Apical cell membrane; Multi-pass membrane protein. Basolateral cell membrane; Multi-pass membrane protein.
蛋白家族：

Cation transport ATPase (P-type) (TC 3.A.3) family, Type IIB subfamily
組織特異性：

Isoforms containing segment B are found in brain, uterus, liver and kidney and in low levels in other tissues. Isoforms containing segment W are found in kidney, uterus, and pancreas. Isoforms containing segment Y are found in pancreas and in low levels i
數據庫鏈接：

KEGG: rno:24215

UniGene: Rn.90982