Recombinant Rat NADPH--cytochrome P450 reductase (Por)

中文名稱：

大鼠Por重組蛋白
貨號(hào)：

CSB-YP018378RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

大鼠Por重組蛋白
貨號(hào)：

CSB-EP018378RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

大鼠Por重組蛋白
貨號(hào)：

CSB-EP018378RA-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Por重組蛋白
貨號(hào)：

CSB-BP018378RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Por重組蛋白
貨號(hào)：

CSB-MP018378RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Por
Uniprot No.：

P00388
別名：

Por; NADPH--cytochrome P450 reductase; CPR; P450R; EC 1.6.2.4
種屬：

Rattus norvegicus (Rat)
蛋白長(zhǎng)度：

Full Length of Mature Protein
表達(dá)區(qū)域：

2-678
氨基酸序列

GDSHEDTSA TMPEAVAEEV SLFSTTDMVL FSLIVGVLTY WFIFRKKKEE IPEFSKIQTT APPVKESSFV EKMKKTGRNI IVFYGSQTGT AEEFANRLSK DAHRYGMRGM SADPEEYDLA DLSSLPEIDK SLVVFCMATY GEGDPTDNAQ DFYDWLQETD VDLTGVKFAV FGLGNKTYEH FNAMGKYVDQ RLEQLGAQRI FELGLGDDDG NLEEDFITWR EQFWPAVCEF FGVEATGEES SIRQYELVVH EDMDVAKVYT GEMGRLKSYE NQKPPFDAKN PFLAAVTANR KLNQGTERHL MHLELDISDS KIRYESGDHV AVYPANDSAL VNQIGEILGA DLDVIMSLNN LDEESNKKHP FPCPTTYRTA LTYYLDITNP PRTNVLYELA QYASEPSEQE HLHKMASSSG EGKELYLSWV VEARRHILAI LQDYPSLRPP IDHLCELLPR LQARYYSIAS SSKVHPNSVH ICAVAVEYEA KSGRVNKGVA TSWLRAKEPA GENGGRALVP MFVRKSQFRL PFKSTTPVIM VGPGTGIAPF MGFIQERAWL REQGKEVGET LLYYGCRRSD EDYLYREELA RFHKDGALTQ LNVAFSREQA HKVYVQHLLK RDREHLWKLI HEGGAHIYVC GDARNMAKDV QNTFYDIVAE FGPMEHTQAV DYVKKLMTKG RYSLDVWS
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.
基因功能參考文獻(xiàn)：
1. The structure of four-electron-reduced, NADP(+)-bound wild type CYPOR shows the plane of the nicotinamide ring positioned perpendicular to the FAD isoalloxazine with its carboxamide group forming H-bonds with N1 of the flavin ring and the Thr535 hydroxyl group. PMID: 29308883
2. the N-terminus of CYP2J2 is very important to form a productive CYP-CPR complex to facilitate electron transfer. PMID: 25450017
3. Data suggest CYP17A1, with electron donor NADPH-P450 reductase, is inherently distributive enzyme but that some processivity is present; some 17alpha-hydroxy pregnenolone formed does not dissociate from CYP17A1 before conversion to dehydroepiandrosterone; CYB5A does not enhance reaction by decreasing k(off) of ligands of CYP17A1. (CYP17A1 = cytochrome P450, family 17, subfamily A, polypeptide 1; CYB5A = cytochrome b5) PMID: 28684414
4. The DeltaGly-143 protein had moderately decreased activity with cytochrome P450 and cytochrome c. It formed a flexible loop, which transiently interacts with the flavin N5, resulting in the generation of both an unstable neutral blue semiquinone and hydroquinone. PMID: 27189945
5. the structure and dynamics of the full-length NADPH-cytochrome P450 oxidoreductase PMID: 24853741
6. Study of the role of P450 hydrophobic residues in complex formation with CPR uncovers novel roles for surface-exposed residues Val267 and Leu270 of CYP2B4 in mediating CYP2B4-CPR interactions. PMID: 21462923
7. Conformational changes of NADPH-cytochrome P450 oxidoreductase are essential for catalysis and cofactor binding. PMID: 21345800
8. Studies indicate that CPR conformational changes involving a combination of a "swinging" and "rotating" model and enables CPR to serve as an effective electron transferring "nano-machine". PMID: 20624491
9. Lipid bilayer has a role in defining the redox potential of the cytochrome P450 reductase flavin domains. PMID: 19908820
10. Importance of the direct NADPH-dependent reduction of superoxide-sensitive probes by cytochrome P450-reductase even though this enzyme does not, on its own accord, produce reactive oxygen species. PMID: 15031143
11. origin of the NOS enzymes from modules consisting of a heme domain and POR or ferredoxin-NADP(+) reductase- and flavodoxin-like subdomains that constitute POR, followed by recruitment of smaller modulating elements into the flavin-binding domains PMID: 16249336
12. cytochrome P450 reductase competes with cytochrome b5 for a binding site on cytochrome P450 2B4 PMID: 17693640
13. We present for the first time evidences that the choroids plexus express transcripts for both UGT1A6 and NADPH-cytochrome P450 reductase PMID: 19023562
14. Purification of of a membrane-bound protein yielded CYPOR PMID: 19152507
15. Bone malformations associated with defective cytochrome P450 oxidoreductase activity are due to reduced cholesterol synthesis and, in turn, reduced Indian hedgehog expression in chondrocytes. PMID: 19264869
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亞細(xì)胞定位：

Endoplasmic reticulum membrane; Single-pass membrane protein; Cytoplasmic side.
蛋白家族：

NADPH--cytochrome P450 reductase family; Flavodoxin family; Flavoprotein pyridine nucleotide cytochrome reductase family
數(shù)據(jù)庫(kù)鏈接：

KEGG: rno:29441

STRING: 10116.ENSRNOP00000001961

UniGene: Rn.11359