Recombinant Rat E3 ubiquitin-protein ligase SIAH1 (Siah1)

中文名稱：

大鼠Siah1重組蛋白
貨號：

CSB-YP846029RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Siah1重組蛋白
貨號：

CSB-EP846029RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Siah1重組蛋白
貨號：

CSB-EP846029RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Siah1重組蛋白
貨號：

CSB-BP846029RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Siah1重組蛋白
貨號：

CSB-MP846029RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Siah1
Uniprot No.：

Q920M9
別名：

Siah1; Siah1aE3 ubiquitin-protein ligase SIAH1; EC 2.3.2.27; RING-type E3 ubiquitin transferase SIAH1; Seven in absentia homolog 1; Siah-1; Siah-1a
種屬：

Rattus norvegicus (Rat)
蛋白長度：

full length protein
表達區域：

1-282
氨基酸序列

MSRQTATALP TGTSKCPPSQ RVPALTGTTA SNNDLASLFE CPVCFDYVLP PILQCQSGHL VCSNCRPKLT CCPTCRGPLG SIRNLAMEKV ANSVLFPCKY ASSGCEITLP HTEKAEHEEL CEFRPYSCPC PGASCKWQGS LDAVMPHLMH QHKSITTLQG EDIVFLATDI NLPGAVDWVM MQSCFGFHFM LVLEKQEKYD GHQQFFAIVQ LIGTRKQAEN FAYRLELNGH RRRLTWEATP RSIHEGIATA IMNSDCLVFD TSIAQLFAEN GNLGINVTIS MC
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (ELL2, MYB, POU2AF1, PML and RBBP8), a cell surface receptor (DCC), cytoplasmic signal transduction molecules (KLF10/TIEG1 and NUMB), an antiapoptotic protein (BAG1), a microtubule motor protein (KIF22), a protein involved in synaptic vesicle function in neurons (SYP), a structural protein (CTNNB1) and SNCAIP. Confers constitutive instability to HIPK2 through proteasomal degradation. It is thereby involved in many cellular processes such as apoptosis, tumor suppression, cell cycle, axon guidance, transcription, spermatogenesis and TNF-alpha signaling. Has some overlapping function with SIAH2. Induces apoptosis in cooperation with PEG3. Upon nitric oxid (NO) generation that follows apoptotic stimulation, interacts with S-nitrosylated GAPDH, mediating the translocation of GAPDH to the nucleus. GAPDH acts as a stabilizer of SIAH1, facilitating the degradation of nuclear proteins. Mediates ubiquitination and degradation of EGLN2 and EGLN3 in response to the unfolded protein response (UPR), leading to their degradation and subsequent stabilization of ATF4.
基因功能參考文獻：
1. Study reveals GAPDH/Siah1 as a novel signaling pathway in apoptosis and function recovery after traumatic spinal cord injury. This signaling pathway in the spinal cord can be blocked by sivelestat sodium. PMID: 27256506
2. The results of this study indicated that CacyBP/SIP is a novel regulator of CRE- and NFAT-driven transcription. PMID: 25163685
3. Data suggest SIAH1 involvement in the injury response of the adult spinal cord of the rats. PMID: 21336655
4. siah-1 Protein is necessary for high glucose-induced glyceraldehyde-3-phosphate dehydrogenase nuclear accumulation and cell death in Muller PMID: 19940145
5. Binds group I metabotropic glutamate receptors and calmodulin; calmodulin binding may play a role in the regulation of group I mGluR function PMID: 11686852
6. These results suggest that Siah-1 might play a role as a regulator of FIH abundance under normoxic conditions. PMID: 18280659
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亞細胞定位：

Cytoplasm. Nucleus. Note=Predominantly cytoplasmic. Partially nuclear.
蛋白家族：

SINA (Seven in absentia) family
數據庫鏈接：

KEGG: rno:140941

STRING: 10116.ENSRNOP00000020329

UniGene: Rn.73937