Recombinant Rat Dual specificity protein phosphatase 1 (Dusp1)

中文名稱：

大鼠Dusp1重組蛋白
貨號：

CSB-YP717559RA
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

大鼠Dusp1重組蛋白
貨號：

CSB-EP717559RA
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

大鼠Dusp1重組蛋白
貨號：

CSB-EP717559RA-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Dusp1重組蛋白
貨號：

CSB-BP717559RA
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Dusp1重組蛋白
貨號：

CSB-MP717559RA
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Dusp1
Uniprot No.：

Q64623
別名：

Dusp1; Cl100; Mkp1; Ptpn16Dual specificity protein phosphatase 1; EC 3.1.3.16; EC 3.1.3.48; Mitogen-activated protein kinase phosphatase 1; MAP kinase phosphatase 1; MKP-1; Protein-tyrosine phosphatase CL100; Protein-tyrosine phosphatase non-receptor type 16
種屬：

Rattus norvegicus (Rat)
蛋白長度：

full length protein
表達區域：

1-367
氨基酸序列

MVMEVGILDA GGLRALLRER AAQCLLLDCR SFFAFNAGHI VGSVNVRFST IVRRRAKGAM GLEHIVPNTE LRGRLLAGAY HAVVLLDERS AALDGAKRDG TLALAAGALC REARSTQVFF LQGGYEAFSA SCPELCSKQS TPMGLSLPLS TSVPDSAESG CSSCSTPLYD QGGPVEILSF LYLGSAYHAS RKDMLDALGI TALINVSANC PNHFEGHYQY KSIPVEDNHK ADISSWFNEA IDFIDSIKDA GGRVFVHCQA GISRSATICL AYLMRTNRVK LDEAFEFVKQ RRSIISPNFS FMGQLLQFES QVLAPHCSAE AGSPAMAVLD RGTSTTTVFN FPVSIPVHPT NSALNYLQSP ITTSPSC
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Dual specificity phosphatase that dephosphorylates MAP kinase MAPK1/ERK2 on both 'Thr-183' and 'Tyr-185', regulating its activity during the meiotic cell cycle.
基因功能參考文獻：
1. Insulin increased while sanguinarine attenuated phosphorylation of MKP-1 at 10 min reperfusion. PMID: 28551880
2. Results suggest that miR-101 has functional significance in the pathophysiology of stress-induced dysfunctions in the ventrolateral orbital cortex. MiR-101 may directly regulate DUSP1 expression, and the mechanism underlying the antidepressant effects of miR-101 may involve the negative regulation of DUSP1 expression, which in turn promotes downstream ERK/BDNF signaling. PMID: 28549965
3. Methylation-mediated silencing of the DUSP-1 promoter does not appear to be associated with reduced expression, indicating the involvement of other factors in specific suppression of DUSP-1 in diabetes-associated cardiac hypertrophy. PMID: 28413926
4. miR-200c plays a role in diabetes-associated cardiac hypertrophy by modulating expression of DUSP-1. PMID: 27696308
5. MKP-1 in the hippocampus might be involved in the etiology of depression, and DNA methylation of MKP-1 gene promoter in the hippocampus did not related with the depression. PMID: 25410305
6. our data show that HuR is a novel target of 15d-PGJ2 and reveal HuR-mediated pre-mRNA processing and mature mRNA stabilization as important regulatory steps in the 15d-PGJ2-induced expression of MKP-1. PMID: 25805336
7. protein phosphatase MKP-1 carries a negative regulation of c-fos gene transcription by dephosphorylation of ERK kinases that are a key signal component under the action of such stress reagent as anisomycin on the E1A + ras-transformed cells PMID: 25474904
8. Data indicate that restraint led to a rapid increase in Mkp1 mRNA within the forebrain and anterior pituitary, and this increase did not require glucocorticoid activity. PMID: 24287620
9. MKP-1 is expressed in dopaminergic neurons cultured from E14 rat ventral mesencephalon. It promotes the growth and elaboration of dopaminergic neuronal processes and can help protect them from the neurotoxic effects of 6-OHDA. PMID: 23584919
10. glucocorticoid-mediated repression of IL-1beta-induced CCL2 gene transcription and protein secretion occurs in part through the upregulation of the MKP-1 gene PMID: 23056550
11. TGFbeta1 induces the expression of MKP-1 in glial cells through the Smad pathway and inhibits MAPK and NF-kappaB signaling, thus revealing a novel mechanism for the neuroprotective actions of TGFbeta1 PMID: 22810097
12. MKP-1, the natural regulator of p-p38, mediates resolution of the spinal cord pro-inflammatory milieu induced by peripheral nerve injury. PMID: 22540262
13. An altered brain renin-angiotensin system influences oxidative stress status and regulates MKP-1 expression in dorsal medulla of transgenic rats. PMID: 22914751
14. MKP-1 significantly inhibits Ang II-stimulated proliferation of vascular smooth muscle cells. Expression of ERK-1 in heart and aorta is higher than that of MKP-1 which dephosphorylates and inactivated ERK-1. PMID: 21179843
15. Small interference RNA-mediated knock-down of MKP-1 enhanced phospho-JNK and neuronal death that is rescued by JNK inhibitor in hypoxia/re-oxygenation. PMID: 22197701
16. Results propose a MIF-NURR1 signaling axis as a regulator of the glucocorticoid sensitivity of MKP1. PMID: 20829434
17. findings show that T3 can antagonize the TNF signaling pathway in pituitary cells by a mechanism that involves the induction of DUSP1 PMID: 20032197
18. Ghrelin's inhibitory effect on gut-derived norepineprine release in sepsis leading to the downregulation of pro-inflammatory cytokines is mediated by MKP-1. PMID: 19956915
19. Osmotic regulation of insulin-induced expression of this enzyme is observed in H4IIE rat hepatoma cells. (MKP-1) PMID: 12529177
20. induction of MKP-1 by hydrogen peroxide is mediated by the MAP kinase-AP-1 pathway; MKP-1 is involved in cellular defense against oxidative stress-induced apoptosis of mesangial cells PMID: 15059639
21. Increase in expression by acute LSD administration of MKP-1 suggests that LSD activates the MAP (mitogen activated protein) kinase pathway PMID: 15255935
22. in pinealocytes, NE activates the beta-adrenergic receptor --> protein kinase A pathway to induce transcription and translation of MKP-1 and the alpha-adrenergic receptor --> PKC pathway to prolong the stimulated responses through stability of the MKP-1 PMID: 15358679
23. findings suggest the involvement of MKP-1 in the regulation of the nocturnal AA-NAT signal PMID: 15527789
24. Anandamide-induced rapid MKP-1 expression switches off MAPK signal transduction in microglial cells activated by stimulation of pattern recognition receptors. PMID: 16387640
25. study reveals that the MKP-1 and MCP-1 as novel mediators of biological effects of Dex may help developing better therapeutic strategies for the treatment of patients with neuroinflammatory diseases. PMID: 17403137
26. These results indicate that MKP-1 modulates the profile of arylalkylamine-n-acetyltransferase activity by selectively shaping the activation profile of p42/44MAPK but not that of p38MAPK. PMID: 17437549
27. Down-regulation of cardioprotective peptide MKP-1, the MAPK pathway negative regulator, in myocardium of streptozotocin-induced diabetic rats, may contribute to the deterioration of cardiac function and lead to diabetic cardiomyopathy. PMID: 17647144
28. The expression of MKP-1 is down-regulated in acute contusive spinal cord injury. PMID: 18630599
29. Induction of mitogen-activated protein kinase phosphatase 1 (MKP-1) by perifused thyrotropin-releasing hormone (TRH) stimulation in rat pituitary GH3 cells. PMID: 18824214
30. Pancreatic-derived cells exposed to reg I grow by activation of signal transduction pathways involving the mitogen-activated protein kinase phosphatases and cyclins, with concomitant induction of MKP-1. PMID: 18929742
31. Induction of MAP kinase phosphatase (MKP)-1 activity by 15-deoxy-delta12,14-prostaglandin J2 (15d-PGJ2) involves the inhibitory action of 15d-PGJ2 on monocyte chemoattractant protein-1 in attenuating inflammatory brain disease. PMID: 19050284
32. These results support a critical role of MKP-1 in TRH-induced, ERK-dependent, prolactin gene expression. PMID: 19289102
33. The abundance of Dusp1 transcripts is > 16-fold higher in the pineal gland, as compared to median expression levels in other tissues. PMID: 19103603
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亞細胞定位：

Nucleus.
蛋白家族：

Protein-tyrosine phosphatase family, Non-receptor class dual specificity subfamily
組織特異性：

Brain. High level expression seen in the cingulate gyrus within the retrospinal cortex, ventral and medial divisions of the anterior thalamus and the medial geniculate nucleus. Expressed at moderate levels in the parietal and temporal cortex. Expressed in
數據庫鏈接：

KEGG: rno:114856

STRING: 10116.ENSRNOP00000005383

UniGene: Rn.98260