Recombinant Rat DNA (cytosine-5)-methyltransferase 3A (Dnmt3a), partial

中文名稱：

大鼠Dnmt3a重組蛋白
貨號：

CSB-YP633132RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

大鼠Dnmt3a重組蛋白
貨號：

CSB-EP633132RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

大鼠Dnmt3a重組蛋白
貨號：

CSB-EP633132RA-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

大鼠Dnmt3a重組蛋白
貨號：

CSB-BP633132RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

大鼠Dnmt3a重組蛋白
貨號：

CSB-MP633132RA
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Dnmt3a
Uniprot No.：

Q1LZ53
別名：

Dnmt3aDNA; cytosine-5)-methyltransferase 3A; Dnmt3a; EC 2.1.1.37
種屬：

Rattus norvegicus (Rat)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Required for genome-wide de novo methylation and is essential for the establishment of DNA methylation patterns during development. DNA methylation is coordinated with methylation of histones. It modifies DNA in a non-processive manner and also methylates non-CpG sites. May preferentially methylate DNA linker between 2 nucleosomal cores and is inhibited by histone H1. Plays a role in paternal and maternal imprinting. Required for methylation of most imprinted loci in germ cells. Acts as a transcriptional corepressor for ZBTB18. Recruited to trimethylated 'Lys-36' of histone H3 (H3K36me3) sites. Can actively repress transcription through the recruitment of HDAC activity. Also has weak auto-methylation activity on Cys-706 in absence of DNA.
基因功能參考文獻：
1. Peripheral nerve injury increases expression of the DNA methyltransferase DNMT3a in the injured DRG neurons via the activation of the transcription factor octamer transcription factor 1. Blocking this increase prevents nerve injury-induced methylation of the voltage-dependent potassium (Kv) channel subunit Kcna2 promoter region and rescues Kcna2 expression in the injured DRG and attenuates neuropathic pain. PMID: 28270689
2. DNMT3A level was upregulated and negatively correlated with miR-200b expression in fibrosis tissues and cardiac fibroblasts. Our results provide a basis for understanding the molecular mechanism by which DNMT3A and miR-200b, in combination with rapamycin, upregulates autophagic activity. PMID: 29786779
3. that increased dorsal horn DNMT3a contributes to bone cancer pain through silencing dorsal horn Kv1.2 expression PMID: 29056068
4. These data suggest that DNMT3a is required for nerve injury-induced and MBD1-mediated epigenetic silencing of the MOR and KOR in the injured DRG. DNMT3a inhibition may serve as a promising adjuvant therapy for opioid use in neuropathic pain management. PMID: 28267064
5. The authors developed a resistant hepatocyte rat model of liver carcinogenesis showing underexpression of DNMT3a and DNMT3b during the early stages. PMID: 25190601
6. We have found that during acute inflammatory pain induced by the application of 10% mustard oil on the tongues of rats, levels of DNMT3a and 3b were elevated markedly PMID: 25416223
7. A severely altered chromatin structure during retinal degeneration coincided with an increased expression of the DNMT isozyme DNMT3a. PMID: 25476906
8. Topiramate prevented ethanol-induced increase in Dnmt3a expression in rate prefrontal cortex. PMID: 24527678
9. Matrix has a profound effect on the regulation of SMC phenotype, which is associated with altered expression, localization of DNMTs and discrete changes DNA methylation PMID: 24282625
10. Steroid hormone exposures may programme lasting differences in amygdala function by altering the expression of the epigenetic factor, DNMT3a. PMID: 21518035
11. results suggest that Dnmt3a expression is subject to dynamic regulation in NAc by two types of chronic emotional stimuli: chronic cocaine use and chronic social defeat stress PMID: 20729844
12. It was concluded that downregulated DNMT3a protein production during Fas-mediated hepatocyte apoptosis results from inhibition of DNMT3a gene transcription. PMID: 19833194
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亞細胞定位：

Nucleus. Chromosome. Cytoplasm.
蛋白家族：

Class I-like SAM-binding methyltransferase superfamily, C5-methyltransferase family
數(shù)據(jù)庫鏈接：

KEGG: rno:444984

STRING: 10116.ENSRNOP00000046524

UniGene: Rn.92659