Recombinant Pyrococcus horikoshii Flap endonuclease 1 (fen)
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中文名稱：掘越氏熱球菌fen重組蛋白
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貨號：CSB-EP008585FHX-B
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說明書：
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規格：
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來源：E.coli
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共軛：Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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其他：
產品詳情
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純度：>85% (SDS-PAGE)
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基因名：fen
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Uniprot No.：
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別名：fen; PH1415Flap endonuclease 1; FEN-1; EC 3.1.-.-; Flap structure-specific endonuclease 1
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種屬：Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3)
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蛋白長度：full length protein
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表達區域：1-343
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氨基酸序列MGVPIGDLVP RKEIDLENLY GKKIAIDALN AIYQFLSTIR QRDGTPLMDS KGRITSHLSG LFYRTINLME AGIKPAYVFD GKPPEFKRKE LEKRREAREE AELKWKEALA KGNLEEARKY AQRATKVNEM LIEDAKKLLQ LMGIPIIQAP SEGEAQAAYM ASKGDVYASA SQDYDSLLFG APRLIRNLTI TGKRKMPGKD VYVEIKPELV VLDEVLKELK ITREKLIELA ILVGTDYNPG GVKGIGPKKA LEIVRYSRDP LAKFQRQSDV DLYAIKEFFL NPPVTNEYSL SWKEPDEEGI LKFLCDEHNF SEERVKNGIE RLKKAIKAGR QSTLESWFVK KKP
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蛋白標簽：Tag?type?will?be?determined?during?the?manufacturing?process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially. -
產品提供形式：Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand. -
復溶：We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
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儲存條件：Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
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保質期：The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C. -
貨期：Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
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注意事項：Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
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Datasheet ：Please contact us to get it.
產品評價
靶點詳情
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功能：Structure-specific nuclease with 5'-flap endonuclease and 5'-3' exonuclease activities involved in DNA replication and repair. During DNA replication, cleaves the 5'-overhanging flap structure that is generated by displacement synthesis when DNA polymerase encounters the 5'-end of a downstream Okazaki fragment. Binds the unpaired 3'-DNA end and kinks the DNA to facilitate 5' cleavage specificity. Cleaves one nucleotide into the double-stranded DNA from the junction in flap DNA, leaving a nick for ligation. Also involved in the base excision repair (BER) pathway. Acts as a genome stabilization factor that prevents flaps from equilibrating into structures that lead to duplications and deletions. Also possesses 5'-3' exonuclease activity on nicked or gapped double-stranded DNA.
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基因功能參考文獻：
- the aromatic groups of Tyr33, Phe79, and Phe278-Phe279 of flap enndonuclease-1 might be involved in the catalytic reaction, probably via multiple stacking interactions with nucleotide bases [flap endonuclease-1] PMID: 14742430
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蛋白家族：XPG/RAD2 endonuclease family, FEN1 subfamily
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數據庫鏈接：
KEGG: pho:PH1415
STRING: 70601.PH1415
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