Recombinant Pseudomonas aeruginosa Type IV major pilin protein PilA (pilA), partial

中文名稱：

銅綠假單胞菌pilA重組蛋白
貨號：

CSB-YP361358EZX
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

銅綠假單胞菌pilA重組蛋白
貨號：

CSB-EP361358EZX-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

銅綠假單胞菌pilA重組蛋白
貨號：

CSB-BP361358EZX
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

銅綠假單胞菌pilA重組蛋白
貨號：

CSB-MP361358EZX
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

pilA
Uniprot No.：

P04739
別名：

pilA; fimA; PA4525; Fimbrial protein; Pilin
種屬：

Pseudomonas aeruginosa (strain ATCC 15692 / PAO1 / 1C / PRS 101 / LMG 12228)
蛋白長度：

Partial
表達區域：

28-149aa
氨基酸序列

PQYQNYVARSEGASALATINPLKTTVEESLSRGIAGSKIKIGTTASTATETYVGVEPDANKLGVIAVAIEDSGAGDITFTFQTGTSSPKNATKVITLNRTADGVWACKSTQDPMFTPKGCDN
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Major component of the type IV pilus (T4P) that plays a role in surface and host cell adhesion, colonization, biofilm maturation, virulence, and twitching, a form of surface-associated motility facilitated by cycles of extension, adhesion, and retraction of T4P fibers. In addition, plays a critical role in type II secretion of exoenzymes by interacting with specific components such as XcpT or XcpU. Modulates host calcium signaling through interaction with host calcium-modulating cyclophilin ligand.
基因功能參考文獻：
1. Glycosylation of type 4 fimbrial protein (PilA) protect Pseudomonas aeruginosa from certain pilus-specific phages. Alterations to PilA sequence can block phage infection, but glycosylation is less likely to create disadvantageous phenotypes. Through construction of chimeric phages, specific phage tail proteins allow infection of P. aeruginosa strains with glycosylated pili. PMID: 29133883
2. PilS's phosphatase motif was required for the autoregulation of pilA transcription, suggesting that under conditions where PilA is abundant, the PilA-PilS interaction promotes PilR dephosphorylation and thus down-regulation of further pilA transcription. PMID: 27162347
3. Data show that the type IV pilus (Tfp)-deficient mutant DeltapilA is severely attenuated in an acute pneumonia model of infection in the lungs of wild-type mice, but is virulent in the lungs of surfactant protein-A SP-A(-/-) mice. PMID: 24080545
4. Found that the calcium-modulating cyclophilin ligand (CAMLG), which is involved in Ca(2+) signaling, was the major host cell PilA binding protein. PMID: 23266901
5. PilA, the major pilin subunit, interacts with PilNOPQ. The results allow us to propose a model whereby PilA makes extensive contacts with the transenvelope complex, possibly to increase local concentrations of PilA monomers for polymerization. PMID: 23457250
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亞細胞定位：

Fimbrium. Cell inner membrane; Single-pass membrane protein. Host endoplasmic reticulum membrane.
蛋白家族：

N-Me-Phe pilin family
數據庫鏈接：

KEGG: pae:PA4525

STRING: 208964.PA4525