E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產(chǎn)品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復(fù)溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲(chǔ)存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項(xiàng):
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet :
Please contact us to get it.
產(chǎn)品評(píng)價(jià)
問(wèn)答及客戶評(píng)論
■ 常見(jiàn)問(wèn)題解答
Q:
Regarding your protein CSB-BP009553PIC, could you please advise on the lead time, the sequencing and the expected tag information? Also, have you made this protein before, and do you know if it is suitable for lyophilization? Thanks!
A:
Very nice to receive your inquiry. Recombinant Phormidium lapideum Glutamine synthetase,partial CSB-BP009553PIC >> Baculovirus Expression Region: 1-23aa; Partial. Tag information: Tag type will be determined during the manufacturing process. The expected tag is N-terminal 6xHis-tagged. Sequence:
TTPQEVLSRIKDQGIKLIDLKFI
Reference: https://www.uniprot.org/uniprot/P20479 Note: At present, this species has not been widely studied, and there is only one region in the Uniprot database, so please confirm whether the sequence listed above is what you want. We haven't expressed this protein before, so the tag type will be determined during the manufacturing process. The expected tag is N-terminal 6xHis-tagged. Proteins are sensitive to heat and lyophilization maximizes retain protein's activity during storage and prolongs shelf life. The possible effects of lyophilization on proteins include: protein partial inactivation, aggregation, and other denaturing problems. However, these negative effects can be minimized by adding protective agents (stabilizers, additives, excipients) and through controlling various conditions of lyophilization. In general, most of the proteins are suitable for lyophilization. Currently there is no data to indicate that this protein is not suitable for lyophilization. Therefore, if you need lyophilized form, please remark when placing the order, we can provide the lyophilization service free of charge. Lead time will be extended for 3 working day. We will add a protective agent (6% trehalose) during the lyophilization.