Recombinant Mycobacterium tuberculosis Large-conductance mechanosensitive channel (mscL), partial

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中文名稱(chēng)：

結(jié)核分枝桿菌mscL重組蛋白
品名簡(jiǎn)稱(chēng)：

Recombinant Mycobacterium tuberculosis mscL protein, partial
貨號(hào)：

CSB-EP358693MVZ1b1
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

mscL
Uniprot No.：

P9WJN5
種屬：

Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
蛋白長(zhǎng)度：

partial
來(lái)源：

E.coli
分子量：

11.5 kDa
表達(dá)區(qū)域：

91-151aa
氨基酸序列

VLPYNTLRKKGEVEQPGDTQVVLLTEIRDLLAQTNGDSPGRHGGRGTPSPTDGPRASTESQ
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Channel that opens in response to stretch forces in the membrane lipid bilayer. The force required to trigger channel opening depends on the nature of the membrane lipids; the presence of phosphatidylinositol enhances mechanosensitivity of the channel. May participate in the regulation of osmotic pressure changes within the cell.
基因功能參考文獻(xiàn)：
1. Results show the interaction of lipids with MscL using atomistic molecular dynamics simulations and identify the simple motif that confers MscL with strong anchoring to the bilayer. PMID: 25437007
2. residues in the primary gate in the first transmembrane domain of MscL are critical for mechanosensitive channel gating PMID: 15111403
3. Data show that solubilization of membrane proteins such as the MscL by covalent attachment of amphiphiles results in homogeneous particles that may prove useful for crystallization, solution NMR spectroscopy, and electron microscopy. PMID: 15465059
4. very efficient hydrophobic matching between the protein and the surrounding lipid bilayer PMID: 15823029
5. binding site of high affinity for anionic phospholipids PMID: 15823046
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亞細(xì)胞定位：

Cell membrane; Multi-pass membrane protein.
蛋白家族：

MscL family
數(shù)據(jù)庫(kù)鏈接：

KEGG: mtu:Rv0985c