Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA (gshA)

中文名稱：

Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA(gshA)
貨號：

CSB-YP368057MVX
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA(gshA)
貨號：

CSB-EP368057MVX
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA(gshA)
貨號：

CSB-EP368057MVX-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA(gshA)
貨號：

CSB-BP368057MVX
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Mycobacterium smegmatis Glutamate--cysteine ligase GshA(gshA)
貨號：

CSB-MP368057MVX
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

egtA
Uniprot No.：

A0R5N1
別名：

egtA; MSMEG_6250; MSMEI_6089Glutamate--cysteine ligase EgtA; EC 6.3.2.2; Gamma-glutamylcysteine synthetase; GCS; Gamma-ECS
種屬：

Mycobacterium smegmatis (strain ATCC 700084 / mc(2)155)
蛋白長度：

full length protein
表達區域：

1-423
氨基酸序列

MALPARSDSG CAVPVEFTSA EQAAAHIGAN SLQDGPIGRV GLEIEAHCFD LSNPTRRPSW DELSAVIADV PPLPGGSRIT VEPGGAVELS GPPYDGPLAA VAALQADRAV LRAEFARRNL GLVLLGTDPL RPTRRVNPGA RYSAMEQFFT ASGTAEAGAA MMTATASVQV NLDAGPRDGW AERVRLAHAL GPTMIAITAN SPMLGGQFTG WCSTRQRVWG QLDSARCGPV LGVDGDDPAS EWARYALRAP VMLVNSPDAV PVTNWVPFAD WADGRAVLGG RRPTEADLDY HLTTLFPPVR PRRWLEIRYL DSVPDALWPA AVFTLTTLLD DPVAAESAAE ATRPVATAWD RAARMGLTDR HLHTAALTCV RLAAERAPAE LEESMTLLMR SVQQRRSPAD DFSDRVVARG IAAAVRELAK GEL
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the synthesis of gamma-glutamylcysteine (gamma-GC). This compound is used as substrate for the biosynthesis of the low-molecular thiol compound ergothioneine.
基因功能參考文獻：
1. The steady-state kinetic parameters of cysteine ligase were determined to be kcat equal to 3.15 s-1, and Km values of 1.8, 0.1, and 0.16 mM for ATP, cysteine, and GlcN-Ins, respectively. PMID: 17848100
蛋白家族：

Glutamate--cysteine ligase type 2 family, EgtA subfamily
數據庫鏈接：

KEGG: msb:LJ00_30905

STRING: 246196.MSMEG_6250