Recombinant Mouse Transcription factor Spi-B (Spib)

中文名稱：

小鼠Spib重組蛋白
貨號：

CSB-YP022568MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Spib重組蛋白
貨號：

CSB-EP022568MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Spib重組蛋白
貨號：

CSB-EP022568MO-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Spib重組蛋白
貨號：

CSB-BP022568MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Spib重組蛋白
貨號：

CSB-MP022568MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Spib
Uniprot No.：

O35906
別名：

Spib; Transcription factor Spi-B
種屬：

Mus musculus (Mouse)
蛋白長度：

Full length protein
表達區(qū)域：

1-267
氨基酸序列

MLALEAAQLD GPHLSCLYPE GVFYDLDSCK PFSYPDSDGG LDSTWGWTEA PPAPAIAPYE AFDPATAAFS HSQTVQLCYS HGPNPSTYSP MGTLDPAPSL EAPGPGLQVY PPEDFTSQTL GSLAYAPYPS PVLSEEEDIM LDSPALEVSD SESDEALLAG SEGRGSEAGA RKKLRLYQFL LGLLLRGDMR ECVWWVEPGA GVFQFSSKHK ELLARRWGQQ KGNRKRMTYQ KLARALRNYA KTGEIRKVKR KLTYQFDSAL LPASRHV
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Sequence specific transcriptional activator which binds to the PU-box, a purine-rich DNA sequence (5'-GAGGAA-3') that can act as a lymphoid-specific enhancer. Promotes development of plasmacytoid dendritic cells (pDCs), also known as type 2 DC precursors (pre-DC2) or natural interferon (IFN)-producing cells. These cells have the capacity to produce large amounts of interferon and block viral replication. Required for B-cell receptor (BCR) signaling, which is necessary for normal B-cell development and antigenic stimulation.
基因功能參考文獻：
1. Mice lacking both PU.1 and SpiB in mature B cells do not generate germinal centers and high-affinity antibody after protein immunization. PU.1 and SpiB double-deficient B cells have a survival defect after engagement of CD40 or Toll-like receptors (TLR), despite paradoxically enhanced plasma cell differentiation. PMID: 29127283
2. Mechanistic analysis indicated that E47 activated expression of the transcription factor Spi-B and the suppressor of cytokine signaling 3 (SOCS3), which both downregulated Foxp3 expression. These findings demonstrate that the balance of Id3 and E47 controls the maintenance of Foxp3 expression in Treg cells and, thus, contributes to Treg cell plasticity. PMID: 27974197
3. Our results suggest that complementary biological functions of PU.1 and Spi-B may be explained by their interaction with a similar set of regions in the genome of B cells. PMID: 25765478
4. Nfkb1 transcriptional activation by PU.1 and Spi-B promotes toll-like receptor-mediated B cell proliferation. PMID: 25733685
5. Data show that transcription factor Spi-B-mediated negative feedback regulation limited medullary thymic epithelial cells (mTECs) development. PMID: 25385757
6. there is a small M-cell population with developmental regulation that is Spi-B independent; however, Spi-B is probably a candidate master regulator of M-cell functional maturation and development by another pathway. PMID: 23212199
7. Data indicate that the developmental defects of plasmacytoid dendritic cells (pDC) in Spi-B-deficient mice were more prominent in the bone marrow. PMID: 23065153
8. The Blnk promoter contains a predicted PU.1 and/or Spi-B binding site that is required for promoter activity and occupied by PU.1 and/or Spi-B. PMID: 22956576
9. RankL-induced expression of SpiB is essential for Lgr5 stem cell-derived epithelial precursors to develop into Peyer's patch M cells PMID: 22778137
10. Spi-B is an essential transcription factor that regulates M-cell differentiation. PMID: 22706340
11. Regulation of follicular B cell differentiation by the related E26 transformation-specific transcription factors PU.1, Spi-B, and Spi-C. PMID: 21057087
12. DNA microarray analyses enabled us to identify two genes inhibited by v-Abl that encode the Igk 3' enhancer-binding transcription factors Spi-B and IRF-4. PMID: 12469118
13. Reduction of Spi-B activity in pro-B cells promotes their differentiation to a stage intermediate between late pro-B cells and large pre-B cells. PMID: 14688320
14. potential positive autoregulatory loop mediated through an upstream regulatory element is essential for proper PU.1 gene expression PMID: 15767686
15. Spi-B function is necessary for efficient development of fetal thymocytes at the CD4-CD8-CD44-CD25+ (DN) checkpoint to the CD4+CD8+ (DP) stage; dysregulation of Spi-B expression results in a dose-dependent perturbation of thymocyte development. PMID: 15879115
16. Maintenance of the B-2 type cell phenotype requires relatively high levels of PU.1, but B-1 cells require little. PMID: 16301746
17. The present findings establish a hierarchy between these two factors and provide a molecular link between OBF-1 and B cell receptor signaling. PMID: 16861304
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亞細胞定位：

Nucleus.
蛋白家族：

ETS family
組織特異性：

Expressed in the medulla of the thymus, the spleen and germinal centers of the lymph nodes. Expressed in B-cells and T-cells, expression increases during B-cell maturation and decreases during T-cell maturation.
數(shù)據(jù)庫鏈接：

KEGG: mmu:272382

STRING: 10090.ENSMUSP00000035539

UniGene: Mm.8012