Recombinant Mouse TBC1 domain family member 1 (Tbc1d1), partial

中文名稱：

小鼠Tbc1d1重組蛋白
貨號：

CSB-YP720191MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Tbc1d1重組蛋白
貨號：

CSB-EP720191MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Tbc1d1重組蛋白
貨號：

CSB-EP720191MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Tbc1d1重組蛋白
貨號：

CSB-BP720191MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Tbc1d1重組蛋白
貨號：

CSB-MP720191MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Tbc1d1
Uniprot No.：

Q60949
別名：

Tbc1d1; Kiaa1108; Tbc1; TBC1 domain family member 1
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

May act as a GTPase-activating protein for Rab family protein(s). May play a role in the cell cycle and differentiation of various tissues. Involved in the trafficking and translocation of GLUT4-containing vesicles and insulin-stimulated glucose uptake into cells.
基因功能參考文獻：
1. Isolated pancreatic islets from D1KO mice exhibited substantially increased glucose-stimulated insulin secretion compared with controls.TBC1D1 exerts its function via a signaling pathway at the level of membrane depolarization. In line, ultrastructural analysis of isolated pancreatic islets revealed both higher insulin-granule density and number of docked granules in beta-cells from D1KO mice compared with WT controls. PMID: 29481597
2. in obese leptin-deficient mice, lack of TBC1D1 has no impact on feeding behavior or energy intake but results in increased energy expenditure, altered energy substrate preference with increased fatty acid oxidation and suppression of obesity; TBC1D1 may have an evolutionary conserved role in regulating energy homeostasis in vertebrates PMID: 27089993
3. Rab28 is a substrate for the GAP domains of both TBC1D1 and TBC1D4 in vitro. PMID: 27929607
4. Tbc1d1 knockout mice have GLUT4 expression and glucose uptake defects in skeletal muscle and adipose tissue. These defects combine in AS160 KO/Tbc1d1 KO mice, supporting nonredundant functions for AS160 and Tbc1d1. PMID: 26625902
5. Findings indicate that the RabGAP TBC1D1 plays a key role in regulating GLUT4 protein levels and in exercise-mediated glucose uptake in nonoxidative muscle fibers. PMID: 25576050
6. TBC1D1-mediated reduction of muscle fatty acid oxidation appears to occur via inhibition of beta-hydroxyaacyl-CoA dehydrogenase activity. PMID: 25163918
7. Data from knockout mice suggest that Tbc1d1 is involved in regulation of energy substrate metabolism (glucose vs. lipids) and energy homeostasis; Tbc1d1 plays important role in susceptibility to diabetes type 2, obesity, and hypertriglyceridemia. PMID: 23892475
8. It was shown that TBC1D1 plays a role in regulation of glucose metabolism in skeletal muscle. Moreover, functional TBC1D1 was required for AICA ribonucleotide- or contraction-induced metabolic responses. PMID: 22693207
9. TBC1D1 Ser237 phosphorylation in skeletal muscle is regulated by alpha2 5AMP-activated protein kinase. PMID: 20837646
10. [review] Knockout mice have been used to probe the specific roles of AMP kinase isoforms in contraction-induced Ser237-Tbc1d1 phosphorylation. PMID: 21078596
11. show that Tbc1d1, a gene known to mediate leanness and linked to obesity, is down-regulated in the absence of TDP-43 PMID: 20660762
12. Contraction causes site-specific phosphorylation of TBC1D1 in skeletal muscle. PMID: 20701589
13. Amino acid substution of TBC1D1 impairs skeletal muscle glucose transport, which could be a mechanism for the obesity associated with this mutation. PMID: 20299473
14. Both Akt and AMPK phosphorylate TBC1D1, but AMPK may be the more robust regulator. PMID: 18276596
15. Mutation of Tbc1d1 suppresses high-fat diet-induced obesity by increasing lipid use in skeletal muscle. PMID: 18931681
16. Genetic evidence to support an important role for AMPK in regulating TBC1D1 in response to physiological stimuli. PMID: 19531644
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亞細胞定位：

Nucleus.
組織特異性：

Expressed in highest levels in hematopoietic cells, testis and kidney.
數據庫鏈接：

KEGG: mmu:57915

STRING: 10090.ENSMUSP00000098756

UniGene: Mm.286353