Recombinant Mouse Sialoadhesin (Siglec1), partial

中文名稱：

小鼠Siglec1重組蛋白
貨號：

CSB-YP723455MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Siglec1重組蛋白
貨號：

CSB-EP723455MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

小鼠Siglec1重組蛋白
貨號：

CSB-EP723455MO-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Siglec1重組蛋白
貨號：

CSB-BP723455MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Siglec1重組蛋白
貨號：

CSB-MP723455MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Siglec1
Uniprot No.：

Q62230
別名：

Siglec1; Sa; Sn; Sialoadhesin; Sheep erythrocyte receptor; SER; Sialic acid-binding Ig-like lectin 1; Siglec-1; CD antigen CD169
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Acts as an endocytic receptor mediating clathrin dependent endocytosis. Macrophage-restricted adhesion molecule that mediates sialic-acid dependent binding to lymphocytes, including granulocytes, monocytes, natural killer cells, B-cells and CD8 T-cells. Preferentially binds to alpha-2,3-linked sialic acid. Binds to SPN/CD43 on T-cells. May play a role in hematopoiesis. May act as a counter-receptor for CLEC10A in lymph node.
基因功能參考文獻：
1. A new subset of resident macrophages in the intestinal muscularis externa was discovered, identified as iba1(pos) CD169(neg). PMID: 27860408
2. the capacity of CD169(+) macrophages to contain the parasite burden and its sequestration into different tissues and to limit infection-induced inflammation is crucial to mitigating Plasmodium infection and pathogenesis PMID: 27477286
3. apoptosis contributes to both tolerance and immunity, as well as establishing CD169 as a critical mediator of the immune response to extracellular vesicles. PMID: 26647968
4. CD169(+) macrophages are important contributors to the IFN-I response and thereby influence antiviral activity, CD8(+) T-cell exhaustion and immunopathology. PMID: 27809306
5. this study shows that binding of antibodies to Sn results in an enhanced phagocytosis of bacteria by mouse macrophages PMID: 27993350
6. Although CD169 participates in the immune response to tumour antigen and appears to be a positive prognostic marker for human cancers, its role in modulating melanoma growth and metastasis is less clear. PMID: 28359758
7. Inhibition of Siglec-1 can prevent atherosclerotic lesion formation by suppress monocytes-endothelial cells adhesion and macrophages accumulation. PMID: 27871915
8. Data show that knockdown of sialic acid binding Ig-like lectin 1 (siglec-1) in RAW 264.7 macrophage resulted in inhibiting the production of transforming growth factor beta 1 (TGF-beta1) production. PMID: 27129263
9. Viral infection significantly upregulated Siglec1 expression in mouse macrophages in an IFN/JAK/STAT1 pathway-dependent manner. PMID: 26358190
10. Siglec-1 is a key receptor for macrophage/lymphocyte trans-infection of surface-bound virions, and the N-acyl side chain of sialic acid is a critical determinant for the Siglec-1/MLV interaction. PMID: 26370074
11. Robust infection in lymph nodes and spleen requires CD169, suggesting that a combination of fluid-based movement followed by CD169-dependent trans-infection can contribute to viral spread. PMID: 26429886
12. CD169(+) monocytes and macrophages have a critical role in preventing excessive inflammation in renal ischemia-reperfusion injury by downregulating intercellular adhesion molecule-1 expression on vascular endothelial cells. PMID: 25266072
13. results identify CD169(+) macrophages as promoters of high-affinity humoral immune responses and emphasize the value of CD169 as target for Ag delivery to improve vaccine responses PMID: 25487358
14. Sialoadhesin is critical for macrophages to phagocytose and clear the sialylated pathogen group B Streptococcus. PMID: 24788876
15. Sn deficiency does not have a significant effect on the severity and progression of lupus nephritis in the NZBWF1 mouse model. PMID: 24286366
16. prion pathogenesis was unaltered in sialoadhesin-deficient mice PMID: 24684244
17. CD169(-/-) mice demonstrated an enhanced response to antigen-pulsed exosomes. This is the first report of a role for CD169 in the capture of exosomes and its potential to mediate the immune response to exosomal antigen. PMID: 24255917
18. Siglec-1 can interact with SR-BI in the phagocytosis of oxLDL by macrophages, rather than act as an independent receptor for oxLDL. PMID: 23520536
19. the CD169/Sn endocytic pathway is conserved and capable of presenting lipid antigens to iNKT cells PMID: 23610394
20. Coculture of activated T effector cells with Sn+ macrophages or Sn+ Chinese hamster ovary cells results in increased cell death, and suggests a regulatory role for Sn-SnL interactions. PMID: 23408841
21. Sn is a potentially important pathogen recognition molecule for systemically introduced sialylated bacteria and is required for rapid production of proinflammatory and type I interferon (IFN) responses. PMID: 22851711
22. The CD169 bleb(+) lymphocytes are enriched for IL-17 committed IL-7Ralpha(hi)CCR6(+) T cells and natural killer cells. PMID: 22675532
23. CD169+ macrophage-depleted mice fail to crossprime tumor-specific CD8 T cells PMID: 21194983
24. Bone marrow CD169+ macrophages promote the retention of hematopoietic stem and progenitor cells in the mesenchymal stem cell niche. PMID: 21282381
25. Sn acts as an endogenous counter-receptor for MGL1 PMID: 15364954
26. Data describe the structure of the N-terminal sialoadhesin domain in complex with a sialic acid-containing heptapeptide, (Ala-Gly-His-Thr(Neu5Ac)-Trp-Gly-His). PMID: 15488769
27. role for sialoadhesin in regulating cells of the immune system rather than in influencing steady-state hematopoiesis PMID: 16449664
28. Results describe the role of the macrophage-restricted sialic acid-binding Ig-like lectin sialoadhesin (Sn, Siglec-1) in the pathogenesis of inherited demyelination in myelin P0(+/-) mice. PMID: 16458537
29. Using flow cytometry of sialoadhesin-transfected cells, antigen recognized by MOMA-1 is sialoadhesin PMID: 16716409
30. Siglecs-1 did not interact with sulfate derivatives of LacNAc and sulfated oligosaccharides containing sialic acid. PMID: 16732727
31. Plays a role in fine tuning the immune response to autoantigens by modulating T cell priming. PMID: 16887986
32. in a primarily genetically caused myelin disorder of the CNS macrophages expressing sialoadhesin partially mediate pathogenesis PMID: 17064921
33. The significant activation of microglia/macrophages observed in the various stages of degeneration in rd1 and rds mouse retinas is not accompanied by Sn expression. PMID: 18641281
34. This indicates that Sn is expressed on mouse alveolar macrophages but that the sialic acid binding activity mediated by this molecule is naturally masked by endogenous sialic acid within the glycocalyx on the cell surface. PMID: 19406152
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亞細胞定位：

[Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted.; [Isoform 3]: Secreted.
蛋白家族：

Immunoglobulin superfamily, SIGLEC (sialic acid binding Ig-like lectin) family
組織特異性：

Detected in lymph node in the subcapsular sinus, interfollicular regions, and T and B-cell boundary (at protein level). Expressed by macrophages in various tissues. Highest expression in spleen and lymph node with lower amounts in lung, liver, bone marrow
數(shù)據(jù)庫鏈接：

KEGG: mmu:20612

STRING: 10090.ENSMUSP00000028794

UniGene: Mm.1374