Recombinant Mouse Serine/threonine-protein kinase WNK4 (Wnk4), partial

中文名稱：

小鼠Wnk4重組蛋白
貨號：

CSB-YP772137MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Wnk4重組蛋白
貨號：

CSB-EP772137MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Wnk4重組蛋白
貨號：

CSB-EP772137MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Wnk4重組蛋白
貨號：

CSB-BP772137MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Wnk4重組蛋白
貨號：

CSB-MP772137MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Wnk4
Uniprot No.：

Q80UE6
別名：

Wnk4; Prkwnk4Serine/threonine-protein kinase WNK4; EC 2.7.11.1; Protein kinase lysine-deficient 4; Protein kinase with no lysine 4
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D, SGK1, TRPV5 and TRPV6. Regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation which appears to prevent membrane trafficking of SLC12A3. Also inhibits the renal K(+) channel, KCNJ1, via a kinase-independent mechanism by which it induces clearance of the protein from the cell surface by clathrin-dependent endocytosis. WNK4 appears to act as a molecular switch that can vary the balance between NaCl reabsorption and K(+) secretion to maintain integrated homeostasis. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition.
基因功能參考文獻：
1. reduced age-dependent weight gain of WNK1 TG mice seems to be related with the decreased Kir6.2 expression via WNK1- and WNK4-regulated protein stability of Kir6.2. PMID: 29392534
2. WNK4 protein affected the DNA-binding ability of C/EBPbeta and thereby reduced PPARgamma expression. In the WNK4(-/-) mice, PPARgamma and C/EBPalpha expression were decreased in adipose tissues, and the mice exhibited partial resistance to high-fat diet-induced adiposity. PMID: 28314693
3. However, phosphorylation of SPAK and NCC at distal convoluted tubules were almost completely absent even in WNK4(-/-)KLHL3(R528H/R528H) mice. In conclusion, increased WNK1 was unable to compensate for WNK4 deficiency and phosphorylate the NCC, indicating that WNK4 is indispensable for the onset of PHAII. PMID: 28743496
4. ENaC and ROMK channel activity in kidney tubules are inhibited in TgWnk4(pseudoaldosteronism type II) mice. Wnk4(PHAII)-induced inhibition of ENaC and ROMK may contribute to the suppression of K(+) secretion in the tubules. PMID: 28365586
5. Accordingly, medullary WNK4 protein levels were significantly increased in the kidneys of KLHL2(-/-) mice. KLHL2 is indeed a physiological regulator of WNK4 in vivo; however, its function might be different from that of KLHL3 because KLHL2 mainly localized in medulla. PMID: 28414128
6. The results indicate that quite modest changes in dietary K intake affect plasma [K] and thiazide-sensitive NaCl cotransporter activity. These effects are mediated largely by WNK4, as this kinase exhibits unique Cl-sensitive properties. PMID: 26422504
7. the increased NCC expression and activation is present in CMA which is highly associated with the enhanced WNK4-SPAK signal pathway using WNK4-/- and SPAK-/- mice. PMID: 26728390
8. increased protein expression levels of WNK1 and WNK4 kinases cause PHAII by KLHL3 R528H mutation due to impaired KLHL3-Cullin3-mediated ubiquitination. PMID: 24821705
9. KLHL3 is phosphorylated at serine 433 in the Kelch domain (a site frequently mutated in hypertension with hyperkalemia) by protein kinase C in cultured cells and that this phosphorylation prevents WNK4 binding and degradation. PMID: 25313067
10. WNK4 is the major positive regulator of NCC in the kidneys. PMID: 24655003
11. WNK1 stimulates the activity of the Na-Cl cotransporter via SPAK, an effect antagonized by WNK4. PMID: 25113964
12. in addition to SPAK and OSR1, WNK4 is able to anchor itself to the N-terminal domain of NKCC1 and to promote cotransporter activation. PMID: 24811174
13. SPAK deficiency corrects pseudohypoaldosteronism II caused by WNK4 mutation. PMID: 24039833
14. Activation of the renal Na+:Cl- cotransporter by angiotensin II is a WNK4-dependent process. PMID: 22550170
15. data suggest that WNK4 inhibits NCC protein through activating the MAPK ERK1/2 signaling pathway. PMID: 22114204
16. these results suggest that these PHAII-causing mutations disrupt a Ca(2+)-sensing mechanism around the acidic motif necessary for the regulation of WNK4 kinase activity by Ca(2+) ions. PMID: 22342722
17. Immunolocalization of WNK4 in mouse kidney. PMID: 21660484
18. Results clearly establish that PHAII caused by the WNK4 D561A mutation is dependent on the activation of the WNK-OSR1/SPAK-NCC cascade. PMID: 21486947
19. beta(2)-adrenergic receptor stimulation led to decreased transcription of the gene encoding WNK4, a regulator of sodium reabsorption PMID: 21499270
20. WNK4 negatively regulates surface expression of NCCT and implicate loss of this regulation in the molecular pathogenesis of an inherited form of hypertension. PMID: 12515852
21. WNK4 has a role in the sodium regulatory pathway of the distal PMID: 12671053
22. general role for WNK4 in the regulation of electrolyte flux in diverse epithelia PMID: 14769928
23. WNK4 reduces transepithelial resistance by increasing absolute chloride permeability at tight junctions. PMID: 15465913
24. mutant WNK4 does not have a dominant effect on the cellular localization of kidney ROMK PMID: 15907795
25. WNK4 interacting with SPAK, which in turn phosphorylates and activates NKCC1 and phosphorylates and deactivates KCC2 PMID: 15930150
26. important differences in the mechanism by which WNK kinases affect expression of NCCT PMID: 16788137
27. Nephron segments that express WNK1-S & WNK4 mRNA have major influence on long-term NaCl reabsorption. WNK pathway responds with significant upregulation of WNK1-S & WNK4 with high K+ intake & reduction in WNK1-S on chronic lowering of K+ or Na+ intake. PMID: 16899520
28. Wnk4 is a molecular switch that regulates the balance between NaCl reabsorption and K+ secretion in distal convoluted tubule PMID: 16964266
29. WNK4 has a key role in coordinating the activities of diverse flux pathways to achieve integrated fluid and electrolyte homeostasis PMID: 17360470
30. an SGK1 site in WNK4 regulates Na+ channel and K+ channel activity PMID: 17360471
31. WNK4 normally inhibits NCC, but this effect is lost by eliminating WNK4 catalytic activity or through PHAII-type mutations PMID: 18701621
32. review: NCC in the distal convoluted tubule plays a critical role in NaCl and, indirectly, in K+ homeostasis; regulation by SPAK/OSR1 kinases and WNKs. PMID: 19008908
33. findings place WNK4 in the signaling pathway between AngII and NCC PMID: 19240212
34. WNK4 inhibits NCC activity by diverting the cotransporter to the lysosome for degradation by way of an AP-3 transport carrier PMID: 19401467
35. Characterization of the kinase activity of a WNK4 protein complex. PMID: 19587141
36. Disruption of the Wnk4 gene decreases phosphorylation of Na-Cl cotransporter, increases Na excretion and lowers blood pressure in a mouse model. PMID: 19633012
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亞細胞定位：

Cell junction, tight junction.
蛋白家族：

Protein kinase superfamily, Ser/Thr protein kinase family, WNK subfamily
組織特異性：

Locates to the distal convoluted tubule, the medullary collecting duct and the cortical collecting duct of the kidney. Expressed in pancreatic duct.
數據庫鏈接：

KEGG: mmu:69847

STRING: 10090.ENSMUSP00000099397

UniGene: Mm.23656