Recombinant Mouse Protein-tyrosine kinase 2-beta (Ptk2b), partial

中文名稱：

小鼠Ptk2b重組蛋白
貨號(hào)：

CSB-YP868757MO
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

小鼠Ptk2b重組蛋白
貨號(hào)：

CSB-EP868757MO
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

小鼠Ptk2b重組蛋白
貨號(hào)：

CSB-EP868757MO-B
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Ptk2b重組蛋白
貨號(hào)：

CSB-BP868757MO
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

小鼠Ptk2b重組蛋白
貨號(hào)：

CSB-MP868757MO
說(shuō)明書：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Ptk2b
Uniprot No.：

Q9QVP9
別名：

Ptk2b; Fak2; Pyk2; RaftkProtein-tyrosine kinase 2-beta; EC 2.7.10.2; Calcium-dependent tyrosine kinase; CADTK; Calcium-regulated non-receptor proline-rich tyrosine kinase; Cell adhesion kinase beta; CAK-beta; CAKB; Focal adhesion kinase 2; FADK 2; Proline-rich tyrosine kinase 2; Related adhesion focal tyrosine kinase; RAFTK
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Non-receptor protein-tyrosine kinase that regulates reorganization of the actin cytoskeleton, cell polarization, cell migration, adhesion, spreading and bone remodeling. Plays a role in the regulation of the humoral immune response, and is required for normal levels of marginal B-cells in the spleen and normal migration of splenic B-cells. Required for normal macrophage polarization and migration towards sites of inflammation. Regulates cytoskeleton rearrangement and cell spreading in T-cells, and contributes to the regulation of T-cell responses. Promotes osteoclastic bone resorption; this requires both PTK2B/PYK2 and SRC. May inhibit differentiation and activity of osteoprogenitor cells. Functions in signaling downstream of integrin and collagen receptors, immune receptors, G-protein coupled receptors (GPCR), cytokine, chemokine and growth factor receptors, and mediates responses to cellular stress. Forms multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. Regulates numerous signaling pathways. Promotes activation of phosphatidylinositol 3-kinase and of the AKT1 signaling cascade. Promotes activation of NOS3. Regulates production of the cellular messenger cGMP. Promotes activation of the MAP kinase signaling cascade, including activation of MAPK1/ERK2, MAPK3/ERK1 and MAPK8/JNK1. Promotes activation of Rho family GTPases, such as RHOA and RAC1. Recruits the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation. Acts as a scaffold, binding to both PDPK1 and SRC, thereby allowing SRC to phosphorylate PDPK1 at 'Tyr-9, 'Tyr-373', and 'Tyr-376'. Promotes phosphorylation of NMDA receptors by SRC family members, and thereby contributes to the regulation of NMDA receptor ion channel activity and intracellular Ca(2+) levels. May also regulate potassium ion transport by phosphorylation of potassium channel subunits. Phosphorylates SRC; this increases SRC kinase activity. Phosphorylates ASAP1, NPHP1, KCNA2 and SHC1. Promotes phosphorylation of ASAP2, RHOU and PXN; this requires both SRC and PTK2/PYK2.
基因功能參考文獻(xiàn)：
1. Pyk2 has a role in spine structure and synaptic function; its deficit contributes to Huntington's disease cognitive impairments PMID: 28555636
2. the non-receptor protein tyrosine kinase 2 beta (PTK2B) plays a critical role in murine beige adipocyte differentiation. PMID: 29750960
3. Data provide evidence that activated PYK2 may function as a component of the microtubule organizing center to regulate spindle assembly during the meiotic process of mouse oocytes. PMID: 28643946
4. results suggest that although Pyk2 and FAK are involved in inflammasome activation, only Pyk2 directly phosphorylates ASC and brings ASC into an oligomerization-competent state by allowing Tyr146 phosphorylation to participate ASC speck formation and subsequent NLRP3 inflammation. PMID: 27796369
5. Osteoprotegerin may induce podosome reassembly and peripheral adhesive structure detachment by modulating phosphorylation of Pyk2 and Src and their intracellular distribution in osteoclasts. PMID: 26743491
6. These results suggest that mechanical stimuli activate P2X7 might induce ECMPs expression through PYK2 except in the case of OPN expression. Altogether, mechanical stimuli-induced ECMPs production might be implicated by extracellular ATP secretion or integrin via PYK2 activation. PMID: 29061307
7. Lineage-tracing of monocyte populations suggests that Pyk2 promotes apoptosis in BM monocytes, thereby acting as an important homeostatic regulator of turnover in these short-lived, innate immune cells. PMID: 28754799
8. Pyk2 selectively contributes to the coordination of phagocytosis-promoting signals downstream of CR3, but is dispensable for FcgammaR-mediated phagocytosis PMID: 26848986
9. the findings suggest a model in which the oocyte is not a passive participant in fertilization, but instead responds to sperm contact by localized PYK2 signaling that promotes actin remodeling events required to physically incorporate the sperm head into the ooplasm. PMID: 28527703
10. results show that Pyk2 contributes to cytotoxic T lymphocyte(CTL) migration by regulating detachment of CTL at the trailing edge, which could explain why Pyk2 is important for chemotactic and migratory responses. PMID: 27456486
11. Defects in Pyk2 suppress Kawasaki Disease-like experimental vasculitis, presumably through CXCL9- and CXCL10-dependent interference with neo-angiogenesis. Since Pyk2-KO mice show no life-threatening phenotype, Pyk2 may be a promising therapeutic molecular target for KD. PMID: 28167306
12. STIM1-induced Ca(2+) signaling activates Pyk2 to inhibit the interaction of VE-PTP and VE-cadherin and hence increase endothelial permeability. PMID: 28385807
13. At fertilization, Pyk2 and p-Pyk2 were detected predominantly in sperm heads and the oocyte cytoplasm. Upon formation of male and female pronuclei, Pyk2 and its activated form accumulate in the two pronuclei. Pyk2 was in blastomere nuclei and the pre-blastula cytoplasm. Pyk2 and its activated form then localized to the perinuclear regions, where blastula cells come into contact with each other. PMID: 27086609
14. Findings indicate the presence of unique and overlapping Pyk2 signaling pathways in osteoblasts, which may be controlled by regulating the phosphorylation, kinase activity, and scaffolding functions of Pyk2. PMID: 26552846
15. Astrocytes from Pyk2-/- mice displayed a slower wound covering as compared with wild-type littermate mice after a mechanical stab lesion, data confirmed in purified astrocytes in vitro; actin polymerization dynamics was altered in Pyk2-/- astrocytes; gelsolin, an actin-capping protein able to interact with Pyk2, was mislocalized in Pyk2-/- migrating astrocytes compared with Pyk2+/+ astrocytes PMID: 26663135
16. Phosphoproteomic analysis identifies FAK2 as a potential therapeutic target for tamoxifen resistance in breast cancer. PMID: 26330541
17. Src has a role in priming Pyk2 (but not FAK) phosphorylation and subsequent activation downstream of integrins PMID: 26866924
18. Resistance to hypoxia-induced PH was markedly higher in Pyk2-deficient mice than in wild-type mice. Pyk2-dependent generation of ROS is necessary for the activation of HIF-1alpha. PMID: 25659487
19. data suggest a novel role for FAK in GPVI-dependent ROS formation and platelet activation and elucidate a proximal signaling role for FAK within the GPVI pathway. PMID: 25415317
20. Cardiomyocyte-specific expression of CRNK, the C-terminal domain of PYK2, maintains ventricular function and slows ventricular remodeling in a mouse model of dilated cardiomyopathy. PMID: 24713463
21. PTK2b was activated during the first few hours after fertilization of the oocyte during the period when anaphase resumption was underway and prior to the pronuclear stage. PMID: 24667605
22. Pyk2 is essential for skin wound reepithelialization in vivo and in vitro and that it regulates epidermal keratinocyte migration via a pathway that requires PKCdelta and MMP functions. PMID: 24598361
23. Data indicate that CD45 functions to regulate Pyk2/FAK activity, likely through the activity of Src family kinases, which in turn regulates the levels of paxillin to modulate macrophage adhesion and migration. PMID: 23936270
24. the important role of Pyk2 in the MK-induced increase in bone volume PMID: 23362087
25. The PYK2 kinase was found to be concentrated and activated at the site of sperm-oocyte interaction, and likely participates in this response. PMID: 23401167
26. PTP-PEST controls actin rearrangement, chemokine-triggered migration, and integrin-induced adhesion, seemingly through Pyk2 PMID: 23589331
27. The expression of Pyk2 is correlated with oxygen-induced retinal neovascularization. PMID: 22800456
28. The results of the present study show the importance of the focal adhesion kinase Pyk2 downstream of G-protein-coupled receptors in supporting platelet aggregation and thrombus formation. PMID: 23175689
29. Pyk2 regulates H+-ATPase-mediated proton secretion in the outer medullary collecting duct via an ERK1/2 signaling pathway. PMID: 22811489
30. We conclude that FAK contributes to cytokine signaling and bone resorption in osteoclasts and partially compensates for the absence of Pyk2 to maintain proper adhesion structures in these cells PMID: 22941736
31. the first study to identify Pyk2 as a substrate for STEP. PMID: 22544749
32. MKs regulate the expression of two alternatively spliced isoforms of Pyk2 in OBs, which may regulate OB differentiation and proliferation. PMID: 22447931
33. the dephosphorylation of Pyk2 requires dynamin's GTPase activity and is mediated by the tyrosine phosphatase PTP-PEST. PMID: 22342188
34. Activation of Pyk2 in the heart during heart failure may contribute to protection against arrhythmia. PMID: 21666110
35. FAK is expressed only in PMA-resistant (or intermediate phenotype) EL4 cells, correlating with enhanced cell-substrate adherence, while Pyk2 is more highly expressed in non-adherent PMA-sensitive cells. PMID: 21533871
36. FAK and Pyk2 have distinct, non-redundant functions in launching mechanical signals during osteoblast mechanotransduction PMID: 21283581
37. Compensatory function of Pyk2 protein in the promotion of focal adhesion kinase (FAK)-null mammary cancer stem cell tumorigenicity and metastatic activity. PMID: 21471206
38. Hypophosphorylated and inactive Pyk2 associates with paxillin at the microtubule organizing center PMID: 21195757
39. Data suggest cell type- and stage-specific roles for PTK2B during early testis development. PMID: 20737507
40. Nonreceptor tyrosine kinase Pyk2 has an important role in the integrin-mediated activation of neutrophils that contributes to normal degranulation responses required for efficient host defense to Staphylococcus aureus infection. PMID: 21187437
41. Pyk2 acts as an early sensor of numerous extracellular signals that trigger a Ca(2+) flux and/or reactive oxygen species to amplify tyrosine phosphorylation signaling events. PMID: 20688918
42. PTP-PEST controls Pyk2 activity and is a positive regulator of secondary T cell activation. PMID: 20727793
43. Studies indicated that the roles of FAK in tumorigenesis are different among the tumor species. PMID: 19458494
44. Elucidating the detailed mechanism by which forces are generated and transmitted in relation to Pyk2 expression and cytoskeletal organization is the basis of this investigation. PMID: 20170630
45. Pyk2, which is essential for inflammatory cell migration in vitro, regulates airway inflammation, Th2 cytokine secretion, and airway hyperresponsiveness in the ovalbumin-sensitized mice during antigen challenge in vivo. PMID: 19520918
46. Pyk2 functions to limit p53 levels, thus facilitating cell growth and survival in a kinase-independent manner. PMID: 19880522
47. regulation of tyrosine phosphorylation by focal adhesion kinase PMID: 11787067
48. Results delineate a cardiomyocyte signaling pathway involving pyk2/related focal adhesion tyrosine kinase (RAFTK) that has potential relevance for cardiac remodeling, focal adhesion reorganization, and loss of contractility. PMID: 12228222
49. role in adhesion-induced association with c-Src, osteoclast spreading, and bone resorption PMID: 12514172
50. Results suggest that proline-rich tyrosine kinase 2 (PYK2) is a regulator of gelsolin, revealing a novel PYK2-gelsolin pathway in regulating actin cytoskeletal organization in multiple cells, including osteoclasts. PMID: 12578912
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亞細(xì)胞定位：

Cytoplasm. Cytoplasm, perinuclear region. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, focal adhesion. Cell projection, lamellipodium. Cytoplasm, cell cortex. Nucleus.
蛋白家族：

Protein kinase superfamily, Tyr protein kinase family, FAK subfamily
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:19229

STRING: 10090.ENSMUSP00000022622

UniGene: Mm.21613