Recombinant Mouse Protein Wnt-4 (Wnt4)

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中文名稱：

小鼠Wnt4重組蛋白
品名簡稱：

Recombinant Mouse Wnt4 protein
貨號：

CSB-EP026137MOa0
說明書：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Wnt4
Uniprot No.：

P22724
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
來源：

E.coli
分子量：

42.6 kDa
表達區(qū)域：

23-351aa
氨基酸序列

SNWLYLAKLSSVGSISEEETCEKLKGLIQRQVQMCKRNLEVMDSVRRGAQLAIEECQYQFRNRRWNCSTLDSLPVFGKVVTQGTREAAFVYAISSAGVAFAVTRACSSGELEKCGCDRTVHGVSPQGFQWSGCSDNIAYGVAFSQSFVDVRERSKGASSSRALMNLHNNEAGRKAILTHMRVECKCHGVSGSCEVKTCWRAVPPFRQVGHALKEKFDGATEVEPRRVGSSRALVPRNAQFKPHTDEDLVYLEPSPDFCEQDIRSGVLGTRGRTCNKTSKAIDGCELLCCGRGFHTAQVELAERCGCRFHWCCFVKCRQCQRLVEMHTCR
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Ligand for members of the frizzled family of seven transmembrane receptors. Plays an important role in the embryonic development of the urogenital tract and the lung. Required for normal mesenchyme to epithelium transition during embryonic kidney development. Required for the formation of early epithelial renal vesicles during kidney development. Required for normal formation of the Mullerian duct in females, and normal levels of oocytes in the ovaries. Required for normal down-regulation of 3 beta-hydroxysteroid dehydrogenase in the ovary. Required for normal lung development and for normal patterning of trachael cartilage rings.
基因功能參考文獻：
1. urinary Wnt4 could be a potential noninvasive biomarker for the early detection of tubular injury. PMID: 27600466
2. GLP-1 promoted adipogenesis through the modulation of the Wnt4/beta-catenin signaling pathway PMID: 27504979
3. Results suggest that the Wnt4 gene encodes signals that are important for various aspects of female reproductive tract development. PMID: 26721931
4. Our functional study revealed that WNT4 molecules were involved in regulating zygotic cleavage and early embryogenesis PMID: 26700598
5. Data indicate that a balance between supporting cell self-renewal and differentiation is maintained in the developing ovary by relative Wnt4 protein/beta-catenin and p27Kip1 protein (p27)/Forkhead box L2 (FOXL2) activities. PMID: 26939755
6. decreased expression of Wnt4 in the aging thymus may be one of the molecular triggers underlying the process of age-related thymic senescence. PMID: 26397044
7. Mir-29c regulates WNT4 signaling. PMID: 26484393
8. Wnt4 is essential to normal mammalian lung development. PMID: 26321050
9. RSPO1, WNT4, and beta-catenin have roles in the signaling pathway during ovarian differentiation in mice. PMID: 25187620
10. The Runx-1 gene can be a Wnt-4 signalling target, and that Runx-1 and Wnt-4 are mutually interdependent in their expression. PMID: 25645944
11. Progesterone and Wnt4 control stem cell function through a luminal-myoepithelial crosstalk with Wnt4 acting independent of progesterone receptor perinatally. PMID: 25603931
12. significant role in supporting thymocyte development and maturation PMID: 24768153
13. results suggest that Wnt4 signaling could be an attractive therapeutic target for treating osteoporosis and preventing skeletal aging PMID: 25108526
14. Data indicate that Wnt4 signaling is necessary during maturation of the ovarian follicle. PMID: 24371124
15. Data from several types of in vitro angiogenesis assays suggest that Wnt4 (but not Wnt1) is a potent inhibitor of capillary outgrowth; Wnt4 and Wnt1 appear to be secreted by vascular endothelial cells into extracellular matrix during angiogenesis. PMID: 22607657
16. Wnt4-null and Rspo1-null pregranulosa cells transition through a differentiated granulosa cell state prior to transdifferentiating towards a Sertoli cell fate. PMID: 24036309
17. Wnt4 signaling through beta-catenin stabilization is sufficient to drive spontaneous myofibroblast differentiation in interstitial pericytes and fibroblasts, emphasizing the importance of this pathway in renal fibrosis. PMID: 23766539
18. Endocardial to myocardial notch1-wnt4-bmp2 axis regulates early heart valve development. PMID: 23560082
19. this study identifies Rspo1 and Wnt4 as two new regulators of cell proliferation in the early gonad regardless of its sex, in addition to the specific role of these genes in ovarian differentiation. PMID: 23095882
20. The repression of many female genes including Wnt4 is necessary for the bipotential gonad to adopt the male testicular fate. PMID: 22705479
21. Our analyses show that Wt1, Sf1, and Wnt4 can be dosage-sensitive in mice, depending on the genetic background, and provide insights into the mechanisms of B6 XY sex reversal PMID: 22496664
22. WNT4 was found to signal via the CTNNB1 pathway in Sertoli cells. PMID: 22253774
23. These data reveal a new role for Wnt4 in mammalian Neuromuscular junction formation. PMID: 22253844
24. Wnt4 regulates steady-state thymic cellularity by a thymic epithelial cell (TEC)-dependent mechanism. The absence of Wnt4 suppressed fetal and postnatal thymic expansion and resulted in decreased TEC numbers. PMID: 21937690
25. Wnt-4 modulates canonical Wnt signaling and acts as regulator of beta-cell proliferation and inflammatory cytokine release. PMID: 21771967
26. An important role of Wnt-4 in thymic senescence, is reported. PMID: 21453014
27. Activation of canonical Wnt signaling was evident as indicated by increased Wnt4 activation in livers of hepatocellular carcinoma farnesoid X-knockout mice. PMID: 21430080
28. These results establish a functional role for non-canonical Wnt signaling in hematopoiesis. PMID: 21541287
29. Wnt4 activated the canonical beta-catenin pathway during myogenic differentiation in both myoblasts and satellite cells and negatively regulated myostatin's expression and its regulating pathways. PMID: 21248078
30. Maintenance of female germ cells hinge upon a delicate balance between positive (WNT4 and beta-catenin) and negative (activin betaB) regulators derived from the somatic cells in the fetal ovary. PMID: 20454446
31. WNT4 is a critical regulator not only of proper postnatal uterine development, but also embryo implantation and decidualization. PMID: 21163860
32. Wnt/beta-catenin signaling occurs in proliferating vascular smooth muscle cells during intimal thickening. This is a result of Wnt4 upregulation. PMID: 21193738
33. Wnt-4 deficiency allows 20% of the germ cells to initiate meiosis in the ovary, whereas meiosis is inhibited completely in the Wnt-4/Wnt-5a double mutant. PMID: 20106871
34. WNT4 is required for normal antral follicle development and may act by regulating granulosa cell functions including steroidogenesis. PMID: 20371632
35. Notch2 overexpression leads to ectopic Wnt4 expression and premature tubule formation. PMID: 20299358
36. data indicate that Wnt4 levels must be regulated in chondrocytes for normal growth plate development and skeletogenesis PMID: 17505543
37. The current results support a view that WNT4 may have a role in oocyte selection and follicle formation and maturation in human ovaries. PMID: 19962424
38. Wnt4(EGFPCre)-mediated inactivation of Wnt4 function considerably reduced the amount of Wnt4 transcripts, led to a severe defect in kidney development, and caused the female embryos to undergo partial sex reversal to males PMID: 19830824
39. role for Wnt-4 in the pathogenesis of renal fibrosis. PMID: 11832423
40. wnt-4 is expressed at distinct stages of follicular development. PMID: 11861511
41. results point to a role for Wnt-4 in adrenal gland development and function PMID: 12399432
42. Wnt4 signaling mediates the increased expression of Dax-1 as the ovary becomes sexually differentiated PMID: 12554773
43. Wnt-4 dependent signaling cascades regulate steroidogenic factor-1-dependent transcription of genes in adrenal cortex PMID: 12732619
44. Data show that the role of WNT4 in gonad development is to pattern the sex-specific vasculature and to regulate steroidogenic cell recruitment. PMID: 12835383
45. Wnt4 mRNA is expressed in a decreasing anterior-to-posterior gradient in the floor plate; commissural axons lacking the Wnt receptor Frizzled3 displayed anterior-posterior guidance defects after midline crossing PMID: 14671310
46. matrilysin may have a role in renal tubular injury and progression of tubulointerstitial fibrosis, and Wnt4 may regulate matrilysin expression in the kidney PMID: 15149334
47. germ cells in the ovarian cortex are almost completely lost in both Wnt4 and follistatin null gonads before birth PMID: 15162500
48. Wnt-4 binds both canonical and noncanonical Frizzled receptors, thereby activating Wnt signaling pathways that may each contribute to kidney tubulogenesis. PMID: 15265686
49. Results identify WNT4 as a new factor involved in the mammalian testis determination pathway and show that genes can have a specific but distinct role in both male and female gonad development. PMID: 15581876
50. Wnt-4 signaling normally acts to suppress testosterone biosynthesis in the female, and that testosterone is the putative mediator of the masculinization phenotype in Wnt-4-deficient females PMID: 15932923
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亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Wnt family
組織特異性：

In adults in lung and brain.
數(shù)據(jù)庫鏈接：

KEGG: mmu:22417

STRING: 10090.ENSMUSP00000036580

UniGene: Mm.20355