Recombinant Mouse Pre-B-cell leukemia transcription factor 1 (Pbx1)

中文名稱：

小鼠Pbx1重組蛋白
貨號：

CSB-YP017505MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Pbx1重組蛋白
貨號：

CSB-EP017505MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Pbx1重組蛋白
貨號：

CSB-EP017505MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Pbx1重組蛋白
貨號：

CSB-BP017505MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Pbx1重組蛋白
貨號：

CSB-MP017505MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Pbx1
Uniprot No.：

P41778
別名：

Pbx1; Pbx-1; Pre-B-cell leukemia transcription factor 1; Homeobox protein PBX1
種屬：

Mus musculus (Mouse)
蛋白長度：

Full length protein
表達區域：

1-430
氨基酸序列

MDEQPRLMHS HAGVGMAGHP GLSQHLQDGA GGTEGEGGRK QDIGDILQQI MTITDQSLDE AQARKHALNC HRMKPALFNV LCEIKEKTVL SIRGAQEEEP TDPQLMRLDN MLLAEGVAGP EKGGGSAAAA AAAAASGGAG SDNSVEHSDY RAKLSQIRQI YHTELEKYEQ ACNEFTTHVM NLLREQSRTR PISPKEIERM VSIIHRKFSS IQMQLKQSTC EAVMILRSRF LDARRKRRNF NKQATEILNE YFYSHLSNPY PSEEAKEELA KKCGITVSQV SNWFGNKRIR YKKNIGKFQE EANIYAAKTA VTATNVSAHG SQANSPSTPN SAGSSSSFNM SNSGDLFMSV QSLNGDSYQG AQVGANVQSQ VDTLRHVISQ TGGYSDGLAA SQMYSPQGIS ANGGWQDATT PSSVTSPTEG PGSVHSDTSN
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Transcription factor which binds the DNA sequence 5'-TGATTGAT-3' as part of a heterodimer with HOX proteins such as HOXA1, HOXA5, HOXB7 and HOXB8. Binds the DNA sequence 5'-TGATTGAC-3' in complex with a nuclear factor which is not a class I HOX protein. Has also been shown to bind the DNA sequence 5'-ATCAATCAA-3' cooperatively with HOXA5, HOXB7, HOXB8, HOXC8 and HOXD4. Acts as a transcriptional activator of PF4 in complex with MEIS1. Also activates transcription of SOX3 in complex with MEIS1 by binding to the 5'-TGATTGAC-3' consensus sequence. In natural killer cells, binds to the NFIL3 promoter and acts as a transcriptional activator of NFIL3, promoting natural killer cell development. Plays a role in the cAMP-dependent regulation of CYP17A1 gene expression via its cAMP-regulatory sequence (CRS1). Probably in complex with MEIS2, is involved in transcriptional regulation by KLF4. Acts as a transcriptional activator of NKX2-5 and a transcriptional repressor of CDKN2B. Together with NKX2-5, required for spleen development through a mechanism that involves CDKN2B repression.; As part of a PDX1:PBX1b:MEIS2B complex in pancreatic acinar cells, is involved in the transcriptional activation of the ELA1 enhancer; the complex binds to the enhancer B element and cooperates with the transcription factor 1 complex (PTF1) bound to the enhancer A element.; (Microbial infection) In complex with PREP1, binds to the 5'-TGATTGAC-3' consensus sequence in the U5 region of Moloney murine leukemia virus and promotes viral transcription.
基因功能參考文獻：
1. uc.40 may play an important role during the differentiation of P19 cells by regulation of PBX1 to promote proliferation and inhibit apoptosis. PMID: 30112697
2. Expression of PBX1 in leukemia cells enhances chemo-resistance and colony formation. PMID: 28034988
3. Pbx-dependent Epithelial-mesenchymal transition programs mediate murine upper lip/primary palate morphogenesis and fusion via regulation of Snail1. PMID: 29437830
4. results indicate Pbx genes act in parallel genetic pathways to orchestrate neuronal subtype differentiation, connectivity, and organization. I PMID: 27568519
5. PBX1 regulates adult neural cell fate determination in a manner beyond that of its heterodimerization partner MEIS2. PMID: 27226325
6. Our results suggest that Pbx1-d impacts lupus development by regulating effector T cell differentiation and promoting follicular helper T cells at the expense of regulatory T cell s and identify Pbx1 as a novel regulator of CD4(+) T cell effector function PMID: 27296664
7. FLT3-ITD is capable of inhibiting FLT3-ITD+ cell proliferation through the p21/Pbx1 axis PMID: 27387666
8. non-cell autonomous functions of Pbx factors within the splenic niche contribute to the regulation of hematopoiesis PMID: 27075259
9. Both histone H3 lysine 27 acetylation and recruitment of the transcription factor Pbx1 at prospective enhancers are regulated by mH2A1.2. PMID: 26832413
10. Thus, PTBP1 controls the activity of Pbx1 to suppress its neuronal transcriptional program prior to induction of neuronal progenitor cells development. PMID: 26705333
11. this study showed that the lupus susceptibility gene Pbx1 not only contributes to autoimmune pathology by impairing T cell tolerance, but also impairs the function of mesenchymal stem cells, which may also contribute to the disease directly or indirectly. PMID: 25416808
12. Pbx1-dependent control of VMC differentiation kinetics underlies gross renal vascular patterning. PMID: 26138478
13. Results thus show how Pbx1 controls Fgf10 in the developing lung. PMID: 24591256
14. These results demonstrate a role for Pbx1 in restraining myeloid maturation while maintaining lymphoid potential to appropriately regulate progenitor reservoirs. PMID: 23660001
15. The Systemic Lupus Erythematosus-associated Pbx1-d isoform acts as a dominant-negative transcriptional regulator. PMID: 22992721
16. In Pbx1-deficient dopaminergic neurons, the high affinity netrin-1 receptor, deleted in colon cancer, is down-regulated. PMID: 22748019
17. Data suggest that PBX1 regulates adipocyte development at multiple levels, promoting the generation of NC-derived APs during embryogenesis, while favoring APs proliferation and preventing their commitment to the adipocyte lineage in postnatal life. PMID: 21922607
18. Pbx1 is a novel lupus susceptibility gene that regulates T cell activation and tolerance. PMID: 22180614
19. PBX1 plays a central role in attenuating the activity of HOXA10 as an activator of osteoblast-related genes. PMID: 21597276
20. Pbx genes share overlapping functions and Pbx1 and Emx2 genetically interact in pelvic formation. PMID: 21455939
21. Scapula development is governed by genetic interactions of Pbx1 with its family members and with Emx2 via their cooperative control of Alx1 PMID: 20627960
22. Chromatin-associated Pbx1 and Hoxa10 were present at osteoblast-related gene promoters preceding gene expression, but only Hoxa10 was associated with these promoters during transcription. PMID: 20439491
23. Pbx1 inactivation disrupts pancreas development and in Ipf1-deficient mice promotes diabetes mellitus PMID: 11912494
24. This protein showed consistent elevation in its levels after retinoic acid treatment in both the forelimbs and hindlimbs. PMID: 12397630
25. homeodomain proteins PBX1 and PREP1 are cellular factors involved in Moloney MLV transcription regulation PMID: 12529389
26. Pbx1 is an essential regulator of mesenchymal function during renal morphogenesis. PMID: 12591246
27. PBX1 complexed with HOXA9 and DNA, so that the posterior Hox hexapeptide adopts an altered conformation. PMID: 12923056
28. Findings establish a Pbx1-dependent pathway that regulates the expansion of steroidogenic factor-1 positive cells essential for adrenal formation and gonadal differentiation and demonstrate an early requirement for Pbx1 in urogenital development PMID: 14595835
29. E2A-PBX1 interacts directly with the KIX domain of CBP/p300 in the induction of proliferation in primary hematopoietic cells PMID: 15507449
30. Data suggest that Pbx1 acts together with multiple Hox proteins in the development of the caudal pharyngeal region, but that some functions of Hox proteins in this region are Pbx1-independent. PMID: 15581866
31. These studies establish a Pbx1-Hox11-dependent genetic and transcriptional pathway in spleen ontogeny. PMID: 15944191
32. evidence for a control of PBX1 intracellular distribution which is independent of MEINOX proteins, but is cell cycle correlated PMID: 16172981
33. Vp16-mediated transformation required the Meis1 function of binding to Pbx and DNA but to its C-terminal domain. PMID: 16648484
34. Pbx1/Pbx2 exert a primary hierarchical function on Hox genes, rather than behaving merely as Hox ancillary factors PMID: 16672333
35. An investigation of the entire Ad4BP/SF-1 gene locus revealed a fetal adrenal enhancer (FAdE) in intron 4 containing highly conserved binding sites for Pbx-Prep, Pbx-Hox, and Ad4BP/SF-1. PMID: 16705164
36. Role of Pbx1 in the maintenance of a functional adrenal cortex mediated by synergistic actions of Pbx1 and Sf-1 in the transcriptional regulation of the critical effector of adrenocortical differentiation, the ACTH receptor. PMID: 17082260
37. Pbx1 critically functions at a stage between hematopoietic stem cell development and B-cell commitment and, therefore, is one of the earliest-acting transcription factors that regulate de novo B-lineage lymphopoiesis. PMID: 17244677
38. Pbx1/Pbx2 govern axial skeletal development by controlling Polycomb and Hox in mesoderm and Pax1/Pax9 in sclerotome. PMID: 18691704
39. Reduction or absence of Pbx2 or Pbx3 leads to Pbx1 haploinsufficiency and specific malformations that resemble tetralogy of Fallot, overriding aorta with ventricular septal defect, and bicuspid aortic valves. PMID: 18723445
40. Pbx1 makes a crucial contribution to distinct regulatory pathways in cardiovascular development. PMID: 18849531
41. Hoxa1-399 reduces Hoxa1-993/Pbx1 binding to the Hoxb1 SOct-R3 region PMID: 19115252
42. PBX1 interactions with HOX proteins and DNA are dispensable for retinoic acid-induced ability of embryonic stem cells to express neural genes and point to a possible involvement of PBX1 in the regulation of imprinted genes. PMID: 19148706
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亞細胞定位：

Nucleus.
蛋白家族：

TALE/PBX homeobox family
組織特異性：

Expressed constitutively in natural killer cell precursors in bone marrow.
數據庫鏈接：

KEGG: mmu:18514

STRING: 10090.ENSMUSP00000135516

UniGene: Mm.43358