Recombinant Mouse Perilipin-5 (Plin5)

中文名稱：

小鼠Plin5重組蛋白
貨號：

CSB-YP804867MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Plin5重組蛋白
貨號：

CSB-EP804867MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Plin5重組蛋白
貨號：

CSB-EP804867MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Plin5重組蛋白
貨號：

CSB-BP804867MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Plin5重組蛋白
貨號：

CSB-MP804867MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Plin5
Uniprot No.：

Q8BVZ1
別名：

Plin5; Lsdp5; Mldp; Oxpat; Pat1; Perilipin-5; Lipid droplet-associated protein PAT-1; Lipid storage droplet protein 5; Myocardial LD protein
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-463
氨基酸序列

MDQRGEDTTL APHSRMSGDQ TAQDPGSSLG ELDQQNVVNR VVALPLVKAT CTAVSSAYNS AKDRHPLLGS ACRLAEHCVC SVTTCALDHA QPLLEHLQPQ LATVNDLACR GLDKLEEKLP FLQQPSDMVV TSAKDTVAKS VTGMVDLAQR GRRWSGELRR SMSQAMDMVL GKSEKLVDRF LPMTEAELAV LAAEAEGPEV GTVEEQRQQQ GYFVRLGSLS ARLRHLAYEH SLGKLRQSKH RTQEMLAQLQ ETLELIQHMQ RGASPSPTFH PPKTQELWGS WSPCLENGRS HSEVELETLA LSRSLTLELQ NAVDALAGCV RGLPPSAQAK VAEVQRSVDA LQATFADAHC LGDVAPTALA EGRGSVARAH ACVDEFLDLV LRAMPLPWLV GPFAPILVEQ SEPLINLATC VDEVVGDPDP RWAHMDWPAQ KRAWEAESAD PGGQEAEPPR GQGKHTMMPE LDF
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Lipid droplet-associated protein that maintains the balance between lipogenesis and lipolysis and also regulates fatty acid oxidation in oxidative tissues. Recruits mitochondria to the surface of lipid droplets and is involved in lipid droplet homeostasis by regulating both the storage of fatty acids in the form of triglycerides and the release of fatty acids for mitochondrial fatty acid oxidation. In lipid droplet triacylglycerol hydrolysis, plays a role as a scaffolding protein for three major key lipolytic players: ABHD5, PNPLA2 and LIPE. Reduces the triacylglycerol hydrolase activity of PNPLA2 by recruiting and sequestering PNPLA2 to lipid droplets. Phosphorylation by PKA enables lipolysis probably by promoting release of ABHD5 from the perilipin scaffold and by facilitating interaction of ABHD5 with PNPLA2. Also increases lipolysis through interaction with LIPE and upon PKA-mediated phosphorylation of LIPE.
基因功能參考文獻：
1. Plin5, a new regulator of myocardial lipid metabolism, decreases free fatty acid peroxidation by inhibiting the lipolysis of intracellular lipid droplets, thus providing cardioprotection against I/R injury and shedding new light on therapeutic solutions for I/R diseases. PMID: 28218306
2. During catecholamine-stimulated lipolysis, Perilipin 5 is phosphorylated by protein kinase A and forms transcriptional complexes with PGC-1alpha and SIRT1 in the nucleus. Perilipin 5 promotes PGC-1alpha co-activator function by disinhibiting SIRT1 deacetylase activity. PMID: 27554864
3. Changes in lipid metabolism resulting from PLIN5 deletion reduce ER stress in muscle, decrease FGF21 production by muscle and liver, and impair glycemic control. PMID: 29378767
4. The data highlight a key role of PLIN5 in lipid droplets function, first by finely adjusting lipid droplets fatty acids supply to mitochondrial oxidation, and second acting as a protective factor against lipotoxicity in skeletal muscle. PMID: 27922115
5. The results indicated that atorvastatin promoted lipolysis and reduced TG accumulation in the liver by increasing PKA-mediated phosphorylation of Plin5. This new mechanism of lipid-lowering effects of atorvastatin might provide a new strategy for NAFLD treatment. PMID: 28919478
6. mitochondria isolated from hearts deficient in Plin5, have specific functional defects PMID: 28811250
7. Plin5 deficiency alters cardiac lipid metabolism and associates with reduced survival following myocardial ischemia. PMID: 27376234
8. Data show that glucose-6-phosphatase and perilipin-5 (G6PC/PLIN5) are upregulated in notch1 knockout (KO) mice. PMID: 27428080
9. PLIN5 is dispensable for normal substrate metabolism during exercise and is not required to promote mitochondrial biogenesis or enhance the cellular adaptations to endurance exercise training. PMID: 27189934
10. High fat diet-induced liver fibrosis is accompanied by an approximate 75% reduction in Plin5 in hepatic stellate cells (HSC), and spontaneous activation of primary HSC produces temporally coincident loss of Plin5 expression and lipid droplet depletion. PMID: 27135793
11. Here, we identify synthetic ligands that release ABHD5 from PLIN1 or PLIN5 without PKA activation and rapidly activate adipocyte and muscle lipolysis. PMID: 26411340
12. the increase in liver PLIN5 during hepatosteatosis drives further lipid accumulation but does not adversely affect hepatic health or insulin sensitivity. PMID: 26296152
13. the elevation of islet PLIN5 during fasting allows partitioning of fatty acids into lipid droplets that is released upon refeeding to support postprandial insulin secretion in cAMP- and GPR40-dependent manners. PMID: 25392244
14. protein kinase A and perilipin 5 interact to regulate cardiac lipolysis PMID: 25418045
15. found that Plin5 expression was increased in steatotic livers PMID: 25179419
16. Results suggest that Plin5 allows excess triacylglycerol accumulation in the hearts of mice with streptozotocin-induced type 1 diabetes. PMID: 24820416
17. Perilipin 5 as a lipid droplet protein adapted to mitochondrial energy utilization PMID: 24535284
18. Plin5 is a novel direct PPARdelta target in muscle PMID: 23606724
19. Findings strongly suggest that Plin5 functions as a lipolytic barrier to protect the cardiac TG pool from uncontrolled TG mobilization and the excessive release of free FAs. PMID: 23345410
20. Perilipin 5 regulates the formation and stabilization of cardiac LDs, and it promotes cardiac steatosis without major heart function impairment. PMID: 23345411
21. Plin5 mRNA and protein levels in the heart are downregulated by loss of Plin4. PMID: 23423172
22. Data show that perilipin 5 (Plin5) knockout mice lacked detectable lipid droplets (LDs), containing significantly less triacylglycerol (TAG) in the heart. detectable LDs, containing significantly less TAG in the heart. PMID: 22532565
23. LSDP5, a novel lipid droplet protein, may contribute to triglyceride accumulation by negatively regulating lipolysis and fatty acid oxidation in hepatocytes. PMID: 22675471
24. A portion of cytosolic perilipin 5 resides in high density lipid droplet complexes that participate in cellular neutral lipid accumulation. PMID: 22063271
25. role of perilipin 5 in mitochondria PMID: 21885430
26. these findings suggest that MLDP is expressed in both differentiating mouse embryonic fibroblasts and stromal-vascular cells by the action of PPARgamma. PMID: 20643096
27. Data show that LSDP5 gene is cloned and expressed in E.coli successfully, which lay the foundation for the related research of this gene. PMID: 19351496
28. MLDP is a novel PAT family protein localized to lipid droplets and enriched in the heart, and regulated by peroxisome proliferator-activated receptor alpha PMID: 16571721
29. we have characterized a novel PAT protein, lipid storage droplet protein 5 (LSDP5) of 463 residues.The LSDP5 gene is primarily transcribed in cells that actively oxidize fatty acids, such as heart, red muscle and liver. PMID: 17234449
30. results indicate that the interaction between Abhd5 and Mldp is dynamic and essential for regulating the activity of Atgl at lipid droplets containing Mldp PMID: 19064991
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亞細胞定位：

Lipid droplet. Cytoplasm. Mitochondrion.
蛋白家族：

Perilipin family
組織特異性：

Highly expressed in oxidative tissues, including heart, liver, brown adipose tissue (BAT) and slow-twitch fibers of skeletal muscle. Lower expression in epididymal white adipose tissue and anterior tibialis and quadriceps. Expressed in adrenal glands. Iso
數據庫鏈接：

KEGG: mmu:66968

STRING: 10090.ENSMUSP00000019808

UniGene: Mm.254985