Recombinant Mouse Msx2-interacting protein (Spen), partial

中文名稱：

小鼠Spen重組蛋白
貨號(hào)：

CSB-YP720303MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Yeast
其他：

中文名稱：

小鼠Spen重組蛋白
貨號(hào)：

CSB-EP720303MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
其他：

中文名稱：

小鼠Spen重組蛋白
貨號(hào)：

CSB-EP720303MO-B
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Spen重組蛋白
貨號(hào)：

CSB-BP720303MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Baculovirus
其他：

中文名稱：

小鼠Spen重組蛋白
貨號(hào)：

CSB-MP720303MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：
來(lái)源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Spen
Uniprot No.：

Q62504
別名：

Spen; Kiaa0929; Mint; Sharp; Msx2-interacting protein; SMART/HDAC1-associated repressor protein; SPEN homolog
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Partial
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

May serve as a nuclear matrix platform that organizes and integrates transcriptional responses. In osteoblasts, supports transcription activation: synergizes with RUNX2 to enhance FGFR2-mediated activation of the osteocalcin FGF-responsive element (OCFRE). Has also been shown to be an essential corepressor protein, which probably regulates different key pathways, such as the Notch pathway. Negative regulator of the Notch pathway via its interaction with RBPSUH, which prevents the association between NOTCH1 and RBPSUH, and therefore suppresses the transactivation activity of Notch signaling. Blocks the differentiation of precursor B-cells into marginal zone B-cells. Probably represses transcription via the recruitment of large complexes containing histone deacetylase proteins. May bind both to DNA and RNA.
基因功能參考文獻(xiàn)：
1. Spenito, a small Spen family member, counteracted Spen function in fat regulation. Finally, mouse Spen and Spenito transcript levels scaled directly with body fat in vivo, suggesting a conserved role in fat liberation and catabolism. This study demonstrates that Spen is a key regulator of energy balance and provides a molecular context to understand the metabolic defects that arise from Spen dysfunction. PMID: 28640815
2. Spen is required for gene repression by Xist. Spen is not required for Xist RNA localization and the recruitment of chromatin modifications. PMID: 26190100
3. Spen is required for Xist-mediated silencing. PMID: 26190105
4. results suggest that Xist silences transcription by directly interacting with SHARP, recruiting SMRT, activating HDAC3, and deacetylating histones to exclude Pol II across the X chromosome PMID: 25915022
5. MINT forms a high affinity complex with CSL; the domains of MINT and CSL that are necessary and sufficient for complex formation are delineated PMID: 21372128
6. MINT plays an important role in tooth development, in particular, epithelial morphogenesis PMID: 20393883
7. Notch1 in concert with Notch2 contributes to the morphogenesis of renal vesicles into S-shaped bodies in a RBP-J-dependent manner. PMID: 19914235
8. Msx2-interacting nuclear target protein (MINT) competed with the intracellular region of Notch for binding to a DNA binding protein RBP-J and suppressed the transactivation activity of Notch signaling. PMID: 12594956
9. Stra13, Dec and Sharp (Mint) bHLH repressors are dynamically regulated during mammary gland development and may function to regulate apoptosis PMID: 15108316
10. MINT enhances Runx2 activation of multiprotein complexes assembled by the OCFRE PMID: 15131132
11. MINT is involved in CRYBP1-mediated Col2a1 gene repression and may play a role in regulation of cartilage development PMID: 15778499
12. Mint deficiency impairs differentiation of early T progenitors into double-negative cells, suggesting that Notch/RBP-J signaling negatively regulates DN1-DN2 transition PMID: 17242367
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亞細(xì)胞定位：

Nucleus. Note=Associates with chromatin.
蛋白家族：

RRM Spen family
組織特異性：

Highly expressed in testis. Expressed at lower level in brain, lung, spleen, liver and kidney. Weakly expressed in cardiac and skeletal muscles and ovary. In spleen, it is expressed in follicular B-cells, while it is weakly expressed in marginal zone B-ce
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:56381

STRING: 10090.ENSMUSP00000101412

UniGene: Mm.299906