Recombinant Mouse Mitogen-activated protein kinase 15 (Mapk15)

中文名稱：

小鼠Mapk15重組蛋白
貨號：

CSB-YP013454MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Mapk15重組蛋白
貨號：

CSB-EP013454MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Mapk15重組蛋白
貨號：

CSB-EP013454MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Mapk15重組蛋白
貨號：

CSB-BP013454MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Mapk15重組蛋白
貨號：

CSB-MP013454MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Mapk15
Uniprot No.：

Q80Y86
別名：

Mapk15; Erk7; Erk8; Mitogen-activated protein kinase 15; MAP kinase 15; MAPK 15; EC 2.7.11.24; Extracellular signal-regulated kinase 7; ERK-7; Extracellular signal-regulated kinase 8; ERK-8
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-549
氨基酸序列

MCAAEVDRHV AQRYLIKRRL GKGAYGIVWK AMDRRTGEVV AIKKIFDAFR DQIDAQRTFR EIMLLKEFGG HPNIIRLLDV IPAKNDRDIY LVFESMDTDL NAVIQKGRLL KDIHKRCIFY QLLRATKFIH SGRVIHRDQK PANVLLDSAC RVKLCDFGLA RSLGDLPEGP GGQALTEYVA TRWYRAPEVL LSSRWYTPGV DMWSLGCILG EMLRGQPLFP GTSTFHQLEL ILKTIPLPSM EELQDLGSDY SALILQNLGS RPQQTLDALL PPDTPPEALD LLKRLLAFAP DKRLSAEQAL QHPYVQRFHC PDREWARESD VRLPVHEGDQ LSAPEYRKRL YQIILEQSGN SRSPREEGLG VVASRAELRA SPARTQSLKS GVLPQVPAET PARKRGPKPP RSPGHDPEHV EVRRQSSDPL FQLPPPGRGE RPPGATGQPP SAPSGVKTQV RAMAPSLTSQ AEAQAANQAL IRSDPARGGG PRAVGARRVP SRLPREAPEP RPGRRMFGIS VSQGAQGAAR AALGGYSQAY GTVCRSALGR LPLLPGPRA
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Atypical MAPK protein that regulates several process such as autophagy, ciliogenesis, protein trafficking/secretion and genome integrity, in a kinase activity-dependent manner. Controls both, basal and starvation-induced autophagy throught its interaction with GABARAP, MAP1LC3B and GABARAPL1 leading to autophagosome formation, SQSTM1 degradation and reduced MAP1LC3B inhibitory phosphorylation. Regulates primary cilium formation and the localization of ciliary proteins involved in cilium structure, transport, and signaling. Prevents the relocation of the sugar-adding enzymes from the Golgi to the endoplasmic reticulum, thereby restricting the production of sugar-coated proteins. Upon amino-acid starvation, mediates transitional endoplasmic reticulum site disassembly and inhibition of secretion. Binds to chromatin leading to MAPK15 activation and interaction with PCNA, that which protects genomic integrity by inhibiting MDM2-mediated degradation of PCNA. Regulates DA transporter (DAT) activity and protein expression via activation of RhoA. In response to H(2)O(2) treatment phosphorylates ELAVL1, thus preventing it from binding to the PDCD4 3'UTR and rendering the PDCD4 mRNA accessible to miR-21 and leading to its degradation and loss of protein expression. Also functions in a kinase activity-independent manner as a negative regulator of growth. Phosphorylates in vitro FOS and MBP. During oocyte maturation, plays a key role in the microtubule organization and meiotic cell cycle progression in oocytes, fertilized eggs, and early embryos. Interacts with ESRRA promoting its re-localization from the nucleus to the cytoplasm and then prevents its transcriptional activity.
基因功能參考文獻：
1. Taken together, our results suggest that ERK8 plays an important role in spindle organization during mouse oocyte meiotic maturation and early embryo cleavage. PMID: 23351492
2. a novel function for ERK8 as a bona fide ERRalpha corepressor, involved in control of its cellular localization by nuclear exclusion, and suggest a key role for this MAP kinase in the regulation of the biological activities of this nuclear receptor. PMID: 21190936
3. Extracellular signal-regulated kinase 8-mediated c-Jun phosphorylation increases tumorigenesis of human colon cancer PMID: 20395206
4. Erk8 has a role as a novel effector of RET/PTC3 and, therefore, RET biological functions PMID: 16484222
亞細胞定位：

Cytoplasm, cytoskeleton, cilium basal body. Cell junction, tight junction. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, centriole. Cytoplasmic vesicle, autophagosome. Golgi apparatus. Nucleus. Cytoplasm. Cytoplasm, cytoskeleton, spindle.
蛋白家族：

Protein kinase superfamily, CMGC Ser/Thr protein kinase family, MAP kinase subfamily
組織特異性：

Expressed at all stages of oocyte meiotic maturation.
數據庫鏈接：

KEGG: mmu:332110

STRING: 10090.ENSMUSP00000087098

UniGene: Mm.40843