E. coli biotin ligase
(BirA) is highly specific in covalently attaching biotin to the 15
amino
acid AviTag peptide. This recombinant protein was biotinylated in
vivo
by AviTag-BirA technology, which method is BriA catalyzes amide
linkage
between the biotin and the specific lysine of the AviTag.
The tag type will
be
determined during production process. If you have specified tag
type, please tell us and we will develop the specified tag
preferentially.
產品提供形式:
Lyophilized powder
Note: We will
preferentially ship the format that we have in stock, however,
if you have any special requirement for the format, please
remark your requirement when placing the order, we will prepare
according to your demand.
復溶:
We recommend that this vial be briefly centrifuged
prior
to opening to bring the contents to the bottom. Please reconstitute
protein in deionized sterile water to a concentration of 0.1-1.0
mg/mL.We recommend to add 5-50% of glycerol (final concentration)
and
aliquot for long-term storage at -20℃/-80℃. Our default final
concentration of glycerol is 50%. Customers could use it as
reference.
儲存條件:
Store at -20°C/-80°C upon receipt, aliquoting is
necessary for
mutiple use. Avoid repeated freeze-thaw cycles.
保質期:
The shelf life is related to many factors, storage
state,
buffer ingredients, storage temperature and the stability of the
protein
itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C.
The
shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期:
Delivery time may
differ from different purchasing way or location, please kindly
consult your local distributors for specific delivery time.
Note: All of our
proteins are default shipped with normal blue ice packs, if you
request to ship with dry ice, please communicate with us in
advance
and extra fees will be charged.
注意事項:
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Receptor for interleukin-33 (IL-33); signaling requires association of the coreceptor IL1RAP. Its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. Possibly involved in helper T-cell function.; Inhibits IL-33 signaling.
基因功能參考文獻:
Taken together, our data provide the evidence that ST2 deficiency in early phase of sepsis downregulates myeloid precursors, inflammatory NK and dendritic cells PMID: 30001716
Studied the association of interleukin 1 receptor-like 1 deficiency (St2) on renal ischaemia-reperfusion injury in euglycaemic and hyperglycaemic mice. PMID: 28916390
sST2 is downregulated in high-metastatic cells compared with low-metastatic human and mouse CRC cells. Knockdown of sST2 in low-metastatic cells enhances tumour growth, metastasis and tumour angiogenesis, whereas its overexpression in high-metastatic cells suppresses these processes. PMID: 27882929
IL-33/ST2 can induce production of proinflammatory cytokines, such as TNF-alpha and IL-6, through production of IL-13 in Plasmodium chabaudi-infected BALB/c mice, suggesting that IL-33/ST2 play a critical role in inflammatory responses to malaria infection. PMID: 28359899
these results provide new insights into the mechanisms by which intestinal epithelial cells , via IL-33/ST2 axis, may control pro-inflammatory TH17 cells in the small intestine to sustain homeostasis PMID: 28198366
Results indicate that IL-33/ST2 signaling is functionally present in primary sensory neurons and contributes to pruritus in poison ivy ACD. Blocking IL-33/ST2 signaling may represent a therapeutic approach to ameliorate itch and skin inflammation related to poison ivy allergic contact dermatitis. PMID: 27821781
CLOCK temporally gates mast cell responses to IL-33 via regulation of ST2 expression. Our findings provide novel insights into IL-33/mast cell-associated physiology and pathologies. PMID: 28259547
an increased prevalence in neonatal mortality was observed in litters from dams lacking ST2 PMID: 29133293
Taken together, the data demonstrate a critical role of MyD88 in DCs and of IL-33 signaling via ST2 in MC903-induced Atopic dermatitis (AD). These data suggest that IL-33/IL-33R may be a therapeutic target of AD. PMID: 28383552
Heligmosomoides polygyrus Alarmin Release Inhibitor (HpARI) prevents binding of active interleukin-33 (IL-33) to the IL-33 receptor. PMID: 29045903
mice deficient in the receptor for IL-33 (Il1rl1-/-) demonstrated enhanced lung clearance of Aspergillus fumigatus PMID: 28784844
This stuidy shown that the mRNA expression of IL-33 and ST2 receptors is increased in the CNS of Rocio virus-infected WT mice and that ST2(-/-) mice showed increased susceptibility to infection. PMID: 27334012
Liver Treg cells show a high expression of ST2, a cellular receptor for tissue alarmin IL-33, which is strongly upregulated in the liver of infected mice. These results illustrate the importance of IL-33 in the suppressive function of liver Treg cells during Cytomegaloviruses (CMVs) infection. PMID: 28448566
These results shed light on endogenous IL-33/ST2 signaling as a potential immune regulatory mechanism that serves to promote beneficial microglial responses and mitigate ischemic brain injury after stroke. PMID: 28389473
Plasmodium berghei infection triggered a dramatic increase of IL-33 expression by oligodendrocytes, through ST2 pathway. PMID: 28448579
Data, including data from studies using knockout mice, suggest that signal transduction via IL33/ST2L (interleukin 33/interleukin-33 receptor) plays a cardioprotective role in myocardial remodeling under mechanical stress (which leads to left ventricular hypertrophy and heart failure). PMID: 28450225
IL-33 acts as a potent immune modulator protecting the liver through activation of ST2(+) Treg cells and control of natural killer cells in immune cell-mediated hepatitis. PMID: 27340126
IL-33/ST2 pathway plays a role in enhancing inflammation and tissue damage at the site of acute inflammation. PMID: 27222019
this study shows that the functional IL-33R is expressed on eosinophil progenitors, basophil progenitors and mast cell progenitors PMID: 27568595
the present study hypothesized that IL-33, as a 'master switch' of tissue repair that is secreted from dying or apoptotic cells, activated the IL-33-ST2-MyD88-TRAF6 pathway, amplified Th2-type responses and was involved in the pulmonary fibrosis process, via its receptor ST2, a stable marker of Th2 cells. PMID: 27358001
ST2 deletion increases inflammatory bone loss in experimental periapical lesions in mice. PMID: 25595464
the results of the present study suggest a possible asthma phenotype that involves the IL-33/ST2 pathway and is important for the development of airway inflammation and AHR in the peripheral airways. PMID: 26609909
Data suggest that IL-33 and ST2 function as a developmental switch to license thermogenesis during the perinatal period. PMID: 27453471
The IL-33/ST2 axis may play a crucial role in the pathogenesis of angiostrongylosis. PMID: 24657070
Blockade of ST2 markedly improves survival of lymphocytic choriomeningitis virus -infected Prf1-/- mice and reduces the severity of multiple disease parameters, including serum levels of IFNgamma. PMID: 26518437
the IL-33/ST2 axis specifically controls visceral adipose tissue-Treg cell development was revealed. PMID: 26277897
The activity of IL-33 at its receptor ST2 is terminated by the formation of two disulphide bridges, resulting in conformational change that disrupts the ST2 binding site. PMID: 26365875
IL-33/ST2 may involve the regulation of ocular immunopathology induced by Toxoplasma gondii infection. PMID: 25693767
ST2/IL-33 signaling contributes to neuropathic pain by activation of astroglial JAK2-STAT3 cascade and neuronal CaMKII-CREB cascade. PMID: 26352378
IL-33 receptor ST2 amplifies the expansion of NK cells and enhances host defense during mouse cytomegalovirus infection. PMID: 25926677
The IL-33/ST2 axis augments effector T-cell responses during acute GVHD. PMID: 25814531
role of the IL-33/ST2 pathway in Plasmodium berghei experimental cerebral malaria development PMID: 25682948
Results indicate that IL-33/ST2 signalling is involved in respiratory syncytial virus (RSV)-induced, Th2-associated airway inflammation but not protective immunity. PMID: 25721734
These results suggest that modulation of the ST2L internalization by FAK/GSK3beta might serve as a unique strategy to lessen pulmonary inflammation. PMID: 25472995
IL-33 promotes ST2-dependent lung fibrosis by the induction of alternatively activated macrophages and innate lymphoid cells in mice PMID: 24985397
ST2 does not impair the establishment of adult L. sigmodontis worms, but is important for the splenic clearance of microfilariae from peripheral blood. PMID: 24663956
Together, these data reveal a cell-intrinsic role for the IL-33/ST2 axis in the regulation of apoptosis in MC, identifying thereby a previously unappreciated pathway supporting expansion of the MC population with inflammation. PMID: 24982172
IL-33/ST2 signalling triggers the production of inflammatory mediators contributing to carrageenin-induced inflammation; this reinforces the importance of IL-33/ST2 signalling as a target in innate inflammation and inflammatory pain PMID: 23347081
These data further suggested that IL-33/ST2 signaling played a vital role in cancer pain. PMID: 23988433
ST2 augments rather than inhibits cytokine release by blood leukocytes and splenocytes exposed to S. pneumoniae or K. pneumoniae, but plays a limited role in host defense during sepsis caused by these pathogens. PMID: 23856919
Which was attenuated in the absence of ST2. PMID: 24105680
The IL-33 and ST2 suppresses Th1 responses in the livers of BALB/c mice infected with Leishmania donovani. PMID: 24045639
Bee venom PLA2-induced innate lymphoid cells & primary Th2 responses were dependent on ST2. The IgE response was largely unaffected by ST2-deficiency, possibly because ST2 is required for primary but not secondary T cell responses. PMID: 24210353
The role of ST2 differs between different target tissues: ST2 is dispensable for the development of Th2 response in the sensitized skin, whereas it is a main inducer of Th2 cytokines in asthmatic airways. PMID: 23633023
These data suggest IL-33 and its ST2 receptor mediate formalin-induced inflammatory pain PMID: 23523996
Mice deficient in IL-33R significantly develop more severe pancreatitis, have greater weight loss, and contain higher viral load compared with wild-type mice. PMID: 23733876
ST2 plays a limited anti-inflammatory role during both primary influenza and postinfluenza pneumococcal pneumonia. PMID: 23483993
T1/ST2 directs Th2 cell activation and polyfunctionality in allergic bronchopulmonary mycosis. PMID: 22990621
Il-33/ST2 activation in HESCS drives an autoinflammatory response that controls the temporal expression of receptivity genes PMID: 23300625
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亞細胞定位:
Cell membrane; Single-pass type I membrane protein.; [Isoform B]: Secreted.
蛋白家族:
Interleukin-1 receptor family
組織特異性:
Predominantly expressed in hematopoietic tissues, and in macrophage, erythroid, epithelial and fibroblast cell lines. Isoform A is expressed in brain astrocytes and microglia. Isoform B is expressed in brain endothelial cells.