Recombinant Mouse Homeobox protein Nkx-2.2 (Nkx2-2)

中文名稱：

小鼠Nkx2-2重組蛋白
品名簡稱：

Recombinant Mouse Nkx2-2 protein
貨號：

CSB-EP015842MO
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Nkx2-2
Uniprot No.：

P42586
別名：

Nkx2-2; Nkx-2.2; Nkx2b; Homeobox protein Nkx-2.2; Homeobox protein NK-2 homolog B
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

46.1kDa
表達區域：

1-273aa
氨基酸序列

MSLTNTKTGFSVKDILDLPDTNDEDGSVAEGPEEESEGPEPAKRAGPLGQGALDAVQSLPLKSPFYDSSDNPYTRWLASTEGLQYSLHGLAASAPPQDSSSKSPEPSADESPDNDKETQGGGGDAGKKRKRRVLFSKAQTYELERRFRQQRYLSAPEREHLASLIRLTPTQVKIWFQNHRYKMKRARAEKGMEVTPLPSPRRVAVPVLVRDGKPCHALKAQDLAAATFQAGIPFSAYSAQSLQHMQYNAQYSSASTPQYPTAHPLVQAQQWTW
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-SUMO-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Transcriptional activator involved in the development of insulin-producting beta cells in the endocrine pancreas. May also be involved in specifying diencephalic neuromeric boundaries, and in controlling the expression of genes that play a role in axonal guidance. Binds to elements within the NEUROD1 promoter.
基因功能參考文獻：
1. The transient hypoxia at P7 altered the expression of Nkx2.2, resulting in delayed myelination in the external capsule. PMID: 28546087
2. This suggests that Nkx2.2 is not only required in the early pancreatic progenitors, but has additional essential activities within the endocrine progenitor population. PMID: 28071588
3. Lmx1a is expressed in enterochromaffin cells and functions downstream of Nkx2.2. PMID: 27287799
4. nuclear import of Nkx2-2 is mediated not only by the classical import pathway but also directly by imp beta1 or imp 13 PMID: 27956177
5. Demonstrate that a rare mature enteroendocrine cell subpopulation that is demarcated by Nkx2.2 expression display stem cell properties during normal intestinal epithelial homeostasis, but is not easily activated upon injury. PMID: 26492922
6. that Nkx2.2 and Nkx2.9 proteins play no role in the development of branchiovisceral motor neurons in hindbrain rostral to rhombomere 4. PMID: 25919494
7. These data identify Nkx2.2 as key regulator to determine neuronal subtypes in the p3 domain of the central nervous system. PMID: 25840610
8. The study demonstrates a previously unrecognized mechanism that controls insulin expression through c-Abl-regulated NKx2.2 and GLUT2. PMID: 24835010
9. Our studies reveal that a subset of mouse perineurial cells are CNS-derived, express Nkx2.2, and are essential for motor nerve development. PMID: 24979729
10. NKX2-2 and MNX1 are etiological genes for neonatal diabetes. PMID: 24411943
11. Nato3 induces ectopic Foxa2-positive cells and indirectly downregulates Nkx2.2 expression. PMID: 24401371
12. Nkx2.2 functions as a major 'switch' to turn off Pdgfra signaling in oligodendrocyte precursor cells and initiate the intrinsic program for oligodendrocyte differentiation. PMID: 24449836
13. Sirt2 enhances CG4 oligodendroglial differentiation and report a novel mechanism through which Nkx2.2 represses CG4 oligodendroglial differentiation via Sirt2. PMID: 21669943
14. The NKX2-2 can induce desired neuronal lineages from most expressing neural progenitor cells by a mechanism resembling developmental binary cell-fate switching. PMID: 21624811
15. Nkx2.2 acts by reinforcing the transcriptional networks initiated by Pax4 and Arx in early committed beta- and alpha-cell, respectively. PMID: 21880149
16. Mutation of the endogenous Nkx2.2 tinman (TN) domain in mice abolishes the interaction between Nkx2.2 and Grg3 and disrupts beta-cell specification PMID: 22056672
17. these experiments identify novel genetic interactions between Nkx2.2 and Arx within the endocrine progenitor cells that ensure the correct specification and regulation of endocrine hormone-producing cells PMID: 21856296
18. sonic hedgehog-GLI1 downstream target genes PTCH1, Cyclin D2, Plakoglobin, PAX6 and NKX2.2 are differently regulated in medulloblastoma and astrocytoma PMID: 21059263
19. Data argue that the Nkx2.2 and Nkx2.9 transcription factors contribute crucially to the formation of neuronal networks that function as central pattern generators for locomotor activity in the spinal cord. PMID: 21068056
20. Nkx2.2 can bind to ghrelin promoter;results suggest upregulation of ghrelin in Nkx2.2 null mice is not due to loss of repression of ghrelin promoter in nonghrelin islet cells; Nkx2.2 may contribute to activation of ghrelin in mature islet epsilon-cells PMID: 19965928
21. co-expression of Nkx2.2 and Nkx6.2 transcription factors in myelinating oligodendrocytes suggests their functional interactions in the regulation of myelin sheath formation and/or maintenance PMID: 19780200
22. Expression profiling of Nkx2.2-/- mice during embryogenesis has allowed identification of known and novel pancreatic genes that function downstream of Nkx2.2 to regulate pancreas development. PMID: 20003319
23. regulates expression of genes selectively expressed in islet beta cells PMID: 12426319
24. role of Pax4 appears to be accomplished via its genetic interaction with another homeobox gene, Nkx2.2 PMID: 14729487
25. Nkx2.2 and Pax4 are required to specify or maintain differentiation of the beta cell fate. PMID: 14970313
26. Data show that FoxA2, Nkx2.2, and PDX-1 regulate islet beta-cell-specific mafA expression through conserved sequences located between base pairs -8118 and -7750 upstream from the transcription start site. PMID: 16847327
27. The control of oligodendrocyte differentiation by Olig2, Sox10 and Nkx2.2 is a dosage-dependent developmental process and can be affected by both haploinsufficiency and over-dosage. PMID: 17098222
28. Nkx2.2 functions predominantly as a transcriptional repressor during specification of endocrine cell types in the pancreas. PMID: 17202186
29. indicate a previously undiscovered role for Nkx2.2 in the maintenance of mature beta-cell function and the formation of normal islet structure PMID: 17456846
30. These studies identify a novel role for Nkx2.2 in intestinal endocrine cell development and reveal the regulatory similarities between cell type specification in the pancreatic islet and in the enteroendocrine population of the intestine. PMID: 18022152
31. During development, Phox2b and Nkx2.2 form a non-cell-autonomous feedback loop that links the neural crest with the pancreatic epithelium, and regulates the size of the beta-cell population. PMID: 18506029
32. These results together imply that Nkx2.2 antisense RNA has a certain biological function which is likely to be not only based on the affect on transcription level of Nkx2.2 coding gene in cis, but also on the other mechanisms. PMID: 18538132
33. NKX2.2 functions in immature endocrine cells to control neuroendocrine differentiation in normal intestine and is expressed in most neuroendocrine tumors of the gut PMID: 18987169
34. These data provide important insights into specific functions of PAX6 and NKX2.2 during glial cell specification that have so far remained largely unexplored. PMID: 19258013
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亞細胞定位：

Nucleus.
蛋白家族：

NK-2 homeobox family
組織特異性：

Expressed in restricted areas of the developing CNS: the hindbrain and forebrain, and pancreas.
數據庫鏈接：

KEGG: mmu:18088

STRING: 10090.ENSMUSP00000069666

UniGene: Mm.330639