Recombinant Mouse Glypican-3 (Gpc3), partial

中文名稱：

小鼠Gpc3重組蛋白
貨號：

CSB-YP804039MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Gpc3重組蛋白
貨號：

CSB-EP804039MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Gpc3重組蛋白
貨號：

CSB-EP804039MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Gpc3重組蛋白
貨號：

CSB-BP804039MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Gpc3重組蛋白
貨號：

CSB-MP804039MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Gpc3
Uniprot No.：

Q8CFZ4
別名：

Gpc3; Glypican-3 [Cleaved into: Glypican-3 alpha subunit; Glypican-3 beta subunit]
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
表達區域：

-
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Cell surface proteoglycan that bears heparan sulfate. Negatively regulates the hedgehog signaling pathway when attached via the GPI-anchor to the cell surface by competing with the hedgehog receptor PTC1 for binding to hedgehog proteins. Binding to the hedgehog protein SHH triggers internalization of the complex by endocytosis and its subsequent lysosomal degradation. Positively regulates the canonical Wnt signaling pathway by binding to the Wnt receptor Frizzled and stimulating the binding of the Frizzled receptor to Wnt ligands. Positively regulates the non-canonical Wnt signaling pathway. Binds to CD81 which decreases the availability of free CD81 for binding to the transcriptional repressor HHEX, resulting in nuclear translocation of HHEX and transcriptional repression. Inhibits the dipeptidyl peptidase activity of DPP4. Plays a role in limb patterning and skeletal development by controlling the cellular response to BMP4. Modulates the effects of growth factors BMP2, BMP7 and FGF7 on renal branching morphogenesis. Required for coronary vascular development. Plays a role in regulating cell movements during gastrulation.
基因功能參考文獻：
1. Coupling of pGPC3 to liposomes was essential for effective priming of GPC3-specific CTLs. PMID: 26616051
2. The expression of GPC-3 was altered by DEN treatment. PMID: 24377529
3. Finding represents a rare four layer genomic overlap consisting of growth associated quantitative trait locus (QTL), body mass associated Gpc3 gene, highly conserved miRNA gene and mature miRNA seed SNP identified in the lean mouse. PMID: 21152117
4. Hepatocyte overexpression of GPC3 suppresses hepatocyte proliferation and liver regeneration and alters gene expression profiles. PMID: 20812357
5. glypican-3 is involved in the recruitment of M2-polarized tumor-associated macrophages in hepatocellular carcinoma PMID: 19838081
6. GPC3 inhibits the PI3K/Akt anti-apoptotic pathway while it stimulates the p38MAPK stress-activated one in murine mammary adenocarcinoma LM3 cells PMID: 19288189
7. Gpc3 function in development of Simpson-Golabi-Behmel syndrome is IGF-independent PMID: 11846487
8. GPC3 knockout mice exhibit alterations in the Wnt signaling pathway, which is also associated with the regulation of cell proliferation PMID: 15537637
9. Polymorphisms in the 3' untranslated region of Gpc3 are strong candidates for the causal sequence variation and loss-of-function mutations can cause overgrowth and developmental abnormalities. PMID: 15799711
10. Altered hematopoiesis in glypican-3-deficient mice results in decreased osteoclast differentiation and a delay in endochondral ossification. PMID: 15936336
11. Zhx2 acts as a repressor of Gpc3 in the adult liver. Gpc3 is activated in the regenerating liver in an Afr2-dependent manner. PMID: 17668883
12. A novel glycosyl phosphatidyl inositol (GPI)-linked proteoglycan, Glypican3 (GPC3, binding to GLUT4 was identified. PMID: 18343214
13. Glypican-3 regulates migration, adhesion and actin cytoskeleton organization in mammary tumor cells through Wnt signaling modulation. PMID: 18404367
14. GPC3 acts as a negative regulator of Hedgehog signaling during mammalian development. PMID: 18477453
15. The heparan sulfate proteoglycan (HSPG) glypican-3 mediates commitment of MC3T3-E1 cells toward osteogenesis. PMID: 19479939
16. The Gpc3-null mice show increased levels of Ihh protein and signalling, but similar levels of Ihh messenger RNA. PMID: 19590577
17. Data show that glypican-3-deficient mice display a high incidence of congenital cardiac malformations like ventricular septal defects, common atrioventricular canal and double outlet right ventricle. PMID: 19733558
顯示更多

收起更多
亞細胞定位：

Cell membrane; Lipid-anchor, GPI-anchor; Extracellular side.
蛋白家族：

Glypican family
組織特異性：

In the developing limb, absent from the apical epidermal ridge at 11 dpc but highly expressed in the underlying mesenchyme. Expression in the mesenchyme at this stage is asymmetric with highest levels in the regions of the distal mesenchyme within the pro
數據庫鏈接：

KEGG: mmu:14734

STRING: 10090.ENSMUSP00000064131

UniGene: Mm.22515