Recombinant Mouse Glutamate decarboxylase 2 (Gad2)

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中文名稱：

小鼠Gad2重組蛋白
品名簡稱：

Recombinant Mouse Gad2 protein
貨號：

CSB-EP009160MO
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP009160MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Gad2.

Based on the SEQUEST from database of E.coli host and target protein, the LC-MS/MS Analysis result of CSB-EP009160MO could indicate that this peptide derived from E.coli-expressed Mus musculus (Mouse) Gad2.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Gad2
Uniprot No.：

P48320
別名：

Gad2; Gad65Glutamate decarboxylase 2; EC 4.1.1.15; 65 kDa glutamic acid decarboxylase; GAD-65; Glutamate decarboxylase 65 kDa isoform
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

69.2kDa
表達區域：

1-585aa
氨基酸序列

MASPGSGFWSFGSEDGSADPENPGTARAWCQVAQKFTGGIGNKLCALLYGDSGKPAEGGGSVTSRAATGKVACTCDQKPCNCPKGDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILTHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMLIARYKMFPEVKEKGMAAVPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEVKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQSCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHIDKCLELAEYLYTIIKNREGYEMVFDGKPQHTNVCFWFVPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Catalyzes the production of GABA.
基因功能參考文獻：
1. These results thus identify a molecularly distinct, orexin-activated lateral hypothalamus submodule that governs physical activity in mice. PMID: 28396414
2. Increased activity due to bicuculline preferentially up-regulated GAD65. This was reduced by AP5, a selective blocker of Ca2+-permeable ion channels and NMDA-Rs, and by U0126, KN93, and K252a, affecting BDNF pathways. PMID: 26241953
3. Data show that miRNAs are involved in regulating the expression of the major type 1 diabetes (T1D) autoantigens IA-2, IA-2beta, and GAD65 enzyme. PMID: 26148972
4. Results show that GAD65 is required for efficient GABAergic synaptic transmission and plasticity, and for maintaining extracellular GABA at a level needed for associative plasticity at cortical inputs in the lateral amygdala PMID: 24663011
5. Menin regulates spinal glutamate-GABA balance through GAD65 contributing to neuropathic pain. PMID: 24905306
6. Our data suggest a role of GAD65-mediated GABA synthesis in the encoding of circadian information to fear memory PMID: 24300892
7. Autoimmunity to GAD65 may play a role in the development of Stiff person syndrome. PMID: 24058450
8. Prenatal and postnatal maternal effects influence GAD65 and GAD67 gene expression depending on the age of the offspring. PMID: 23876745
9. These findings indicate novel, differential roles for GluN2A, B and D receptors and for GAD65-mediated GABA in the regulation of individual topographies of orofacial movement PMID: 23892010
10. SMAR1 and p53 act synergistically to up-regulate GAD65 expression upon Streptozotocin treatment. PMID: 22978699
11. Data show that chronic pain epigenetically suppresses Gad2 transcription through histone deacetylase-mediated histone hypoacetylation, resulting in impaired gamma-aminobutyric acid synaptic inhibition. PMID: 21983856
12. These results suggest that modulation of the synaptic transmission from primary afferents to SG neurons by group III mGluR agonist is specific to the type of nociceptive primary afferents but not to the type of target neurons. PMID: 21177998
13. GAD65 is crucial for maintenance of biosynthesis of synaptic GABA particularly by direct synthesis from astrocytic glutamine via glutamate. PMID: 20664610
14. An impaired tonic inhibition in GAD65-deficient mice is revealed demonstrating a significant role of GAD65 in the synthesis of GABA acting extrasynaptically. PMID: 21039523
15. Mice possessing a monoclonal GAD65-specific CD4-positive T cell population develop a lethal encephalomyelitis-like disease in the absence of any other T cells or B cells. PMID: 20348424
16. Deletion of Gad2 reduced the effect of flurazepam on motor incoordination and increased the effect of extrasynaptic GABAA receptor agonist gabaxadol without changing the duration of loss of righting reflex produced by these drugs. PMID: 20002022
17. GAD65 plays an important role in regulating fear responses during extinction of cued but apparently not contextual fear. PMID: 20016086
18. Absence of long-term depression in the visual cortex of GAD65 knock-out mice PMID: 12097476
19. CD4+ T cells from glutamic acid decarboxylase (GAD)65-specific T cell receptor transgenic mice are not diabetogenic and can delay diabetes transfer PMID: 12186840
20. Induction of autoimmune diabetes through insulin (but not this enzyme)DNA vaccination in NOD mice PMID: 12401715
21. No significant GAD65 gene expression differences are detected in either drug-naive or acute ethanol withdrawn mouse brain. PMID: 12691782
22. Results indicate that glutamic acid decarboxylase (GAD65) is not an essential autoantigen in the pathogenesis of diabetes. PMID: 12796471
23. Selectively altered behavioral fear response in GAD65 deficient mice, most likely due to deficits in threat estimation or elicitation of appropriate conditioned fear behavior. GAD65 is a genetic determinant of conditioned fear behavior. PMID: 12884963
24. The cumulative incidence of autoimmune diabetes was found not to be influenced by GAD65 deficiency in NOD mice. PMID: 14676944
25. These results suggest that GAD65-generated GABA is implicated more complex processing of taste information including taste mixtures and palatability may be finely tuned by GAD65-mediated GABA synthesis. PMID: 15036622
26. GAD65 knockout mice showed robust deficits in prepulse inhibition which were reversed by the atypical antipsychotic agent clozapine PMID: 15114343
27. Similar to the rat at P5, mice at P6 are unable to sustain an increase in CO2 induced respiratory frequency and GAD65 contributes to this fall off. PMID: 15134589
28. P boutons can be distinguished from other GABAergic terminals in the ventral horn of rat and mouse spinal cord by their high level of the glutamic acid decarboxylase (GAD) 65 isoform of GAD. PMID: 15947074
29. lack of gad65 impairs the function of the respiratory network in mouse fetuses and the impairment progresses with fetal age PMID: 17418495
30. GAD65 plays a major role in GABA transmission in normal physiological condition. PMID: 17482148
31. GAD65-mediated GABA synthesis is critical for the consolidation of stimulus-specific fear memory PMID: 18323571
32. anti-whole GAD65-reactive T cells have the ability to regulate the development of type 1 diabetes PMID: 19188044
33. There are reduced NMDA NR2A levels and slower NMDA currents in visual cortex of GAD65 knockout mice PMID: 19406876
34. study found that the number of somatic GAD65-puncta on individual layer 2/3 pyramidal neurons is reduced when mice are dark-reared from birth and reverted to normal levels by re-exposure to light PMID: 19503840
35. GAD65-mediated gamma-aminobutyric acid (GABA) synthesis plays relatively small but significant roles in nociceptive processing via supraspinal mechanisms. PMID: 19571163
36. MHC class I-restricted responses to GAD65 provide an inflammatory focus for the generation of islet-specific pathogenesis and beta cell destruction, as well as a therapeutic role for GAD65 peptide determinants in type I diabetes. PMID: 11466400
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亞細胞定位：

Cytoplasm, cytosol. Cytoplasmic vesicle. Cell junction, synapse, presynaptic cell membrane; Lipid-anchor. Golgi apparatus membrane; Peripheral membrane protein; Cytoplasmic side.
蛋白家族：

Group II decarboxylase family
數據庫鏈接：

KEGG: mmu:14417

STRING: 10090.ENSMUSP00000028123

UniGene: Mm.4784