Recombinant Mouse Forkhead box protein L2 (Foxl2)

中文名稱：

小鼠Foxl2重組蛋白
貨號：

CSB-YP008827MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Foxl2重組蛋白
貨號：

CSB-EP008827MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Foxl2重組蛋白
貨號：

CSB-EP008827MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Foxl2重組蛋白
貨號：

CSB-BP008827MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Foxl2重組蛋白
貨號：

CSB-MP008827MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Foxl2
Uniprot No.：

O88470
別名：

Foxl2; PfrkForkhead box protein L2; Pituitary forkhead factor; P-Frk
種屬：

Mus musculus (Mouse)
蛋白長度：

Full length protein
表達區域：

1-375
氨基酸序列

MMASYPEPED TAGTLLAPES GRAVKEAEAS PPSPGKGGGT TPEKPDPAQK PPYSYVALIA MAIRESAEKR LTLSGIYQYI IAKFPFYEKN KKGWQNSIRH NLSLNECFIK VPREGGGERK GNYWTLDPAC EDMFEKGNYR RRRRMKRPFR PPPAHFQPGK GLFGSGGAAG GCGVPGAGAD GYGYLAPPKY LQSGFLNNSW PLPQPPSPMP YASCQMAAAA AAAAAAAAAA GPGSPGAAAV VKGLAGPAAS YGPYSRVQSM ALPPGVVNSY NGLGGPPAAP PPPPPPPHPH PHPHAHHLHA AAAPPPAPPH HGAAAPPPGQ LSPASPATAA PPAPAPTSAP GLQFACARQP ELAMMHCSYW DHDSKTGALH SRLDL
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Transcriptional regulator. Critical factor essential for ovary differentiation and maintenance, and repression of the genetic program for somatic testis determination. Prevents trans-differentiation of ovary to testis through transcriptional repression of the Sertoli cell-promoting gene SOX9. Has apoptotic activity in ovarian cells. Suppresses ESR1-mediated transcription of PTGS2/COX2 stimulated by tamoxifen. Activates SIRT1 transcription under cellular stress conditions. Activates transcription of OSR2. Is a regulator of CYP19 expression. Is a transcriptional repressor of STAR. Participates in SMAD3-dependent transcription of FST via the intronic SMAD-binding element.
基因功能參考文獻：
1. We found that endometrial cells expressing low FOXL2 levels, either endogenous or genetically manipulated, were associated with a higher attachment rate of mouse blastocysts. low-FOXL2 levels were associated with changes in the expression level of components of the Wnt/Fzd and apoptotic pathways, both of which are involved in uterine receptivity. PMID: 29415736
2. Testis determination involves FGFR2c-mediated repression of both the WNT4- and FOXL2-driven ovarian-determining pathways. PMID: 28938467
3. direct overexpression of Foxl2 decreased the expression of Sertoli cell-specific genes in primary Sertoli cells. Taken together, these results demonstrate that repression of beta-catenin (CTNNB1) signaling is required for lineage maintenance of Sertoli cells. PMID: 28900034
4. evidence that FOXL2 modulates Col1a2 transcription through interaction with a response element 65 Kb upstream of the transcription start site PMID: 27212026
5. Data indicate that the hypothalamic-pituitary-gonadal axis controls expression of Foxl2 in pituitary gonadotropes primarily through positive feedback from ovarian hormones. PMID: 25955311
6. FOXL2 mobilizes estrogen signaling to establish a coherent feed-forward loop repressing Sox9. PMID: 25369636
7. Foxl2 has a crucial role in postnatal uterine maturation and could help to understand sub-fertility predisposition in women. PMID: 25687138
8. Results support FOXL2 as a master transcription factor in a spectrum of developmental processes, including growth, cartilage and bone formation. Its action overlaps that of SOX9, though they are antagonistic in female vs male gonadal sex determination but conjoint in cartilage and skeletal development. PMID: 26134413
9. Microarray expression profiling of whole adrenal mRNA from ovariectomized vs. intact mice demonstrated selective upregulation of gonadal-like genes including Spinlw1 and Insl3 in GDX-induced adrenocortical tumors of the mouse. PMID: 25289806
10. our results demonstrate the necessity of FOXL2 for proper FSH production in mice and implicate FOXL2 in integration of transcription factors at the level of the FSHbeta promoter. PMID: 25105693
11. Results show that a piggyBac insertion ~160 kb upstream of the transcription start site of Foxl2 partially disrupted its expression resulting in BPES (Blepharophimosis, ptosis, epicanthus inversus syndrome)-like conditions. PMID: 24565867
12. AMH is an endogenous target gene of FOXL2. AMH and FOXL2 collaboratively work to reserve ovarian follicles. PMID: 24973035
13. When the Smad4 gene is ablated in combination with its DNA binding cofactor Foxl2 in gonadotrope cells, mice make essentially no FSH and females are sterile. PMID: 24739304
14. Differential proximal promoter DNA methylation may contribute to cell- specific Foxl2 expression in some cellular contexts. PMID: 24098544
15. results demonstrate that miR-133b down-regulates Foxl2 expression in granulosa cells by directly targeting the 3'UTR PMID: 23810756
16. FOXL2 is required for FSH synthesis in vivo. PMID: 23340250
17. Increasing expression of the ovarian marker FOXL2 in mutant cords starting at E15.5, indicating progressive transdifferentiation of mutant Sertoli cells. PMID: 22837482
18. Data suggest that forkhead L2 plays a role in down-regulation of Star, Cyp19a1, Cyp11a1, and Ccnd2 genes in granulosa cells under influence of follicle stimulating hormone (or testosterone). PMID: 22847492
19. Protein-protein interactions among FOXL2, SMAD3, and progesterone receptor (PR) recruited to the Fshb promoter play a key role in facilitating Fshb transcription before the secondary FSH surge in rodents. PMID: 22294749
20. Data suggest that Foxl2 is involved in preventing ectopic steroidogenesis in foetal ovaries. PMID: 22116255
21. These data indicate that, in addition to its role in the ovary, FoxL2 function in the pituitary is required for normal expression of FSH. PMID: 21700720
22. SMADs and FOXL2 synergistically regulate murine follicle stimulating hormone beta transcription via a conserved proximal promoter element PMID: 21622537
23. FOXL2 is expressed in ovary and adrenal gland; FOXL2 represses the promoter activity of Star; FOXL2 and NR5A1 synergistically up-regulate the transcriptional activity of Mc2r PMID: 20650879
24. These data support a model in which FOXL2 and BMP2 cooperate to ensure correct expression of Fst in the developing ovary. PMID: 21084449
25. FoxL2 plays a key role in activin induction of the FSHbeta gene, by binding to sites conserved across multiple species. PMID: 20233786
26. Following Foxl2 loss, early testis genes and several novel ovarian genes were consistently dysregulated during embryo-fetal development. PMID: 19875493
27. Molecular evolution and expression of FOXL2 PMID: 12471206
28. FOXL2 is a highly conserved early regulator of vertebrate ovarian development. PMID: 12810580
29. Foxl2 action thus represses the male gene pathway at several stages of female gonadal differentiation PMID: 15944199
30. FOXL2 plays a role in pituitary developmental control and in regulation of Cga gene expression. PMID: 16840539
31. FoxL2 and Smad3 coordinately regulate follistatin gene transcription.( PMID: 19106105
32. The results support the proposal of dose-dependent Foxl2 function and anti-testis action throughout ovary differentiation. PMID: 19538736
33. Study demonstrates in the mouse that a single factor, the forkhead transcriptional regulator FOXL2, is required to prevent transdifferentiation of an adult ovary to a testis. PMID: 20005806
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亞細胞定位：

Nucleus.
組織特異性：

Expressed in the mesenchyme of developing eyelids. Expressed in ovaries throughout development and adulthood, localized to the undifferentiated granulosa cells in small and medium follicles as well as cumulus cells of preovulatory follicles. Expressed in
數據庫鏈接：

KEGG: mmu:26927

STRING: 10090.ENSMUSP00000053297

UniGene: Mm.151239