Recombinant Mouse F-box only protein 32 (Fbxo32)

中文名稱：

小鼠Fbxo32重組蛋白
貨號：

CSB-YP887410MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

小鼠Fbxo32重組蛋白
貨號：

CSB-EP887410MO-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Fbxo32重組蛋白
貨號：

CSB-BP887410MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Fbxo32重組蛋白
貨號：

CSB-MP887410MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Fbxo32
Uniprot No.：

Q9CPU7
別名：

Fbxo32F-box only protein 32; Atrogin-1; Muscle atrophy F-box protein; MAFbx
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達(dá)區(qū)域：

1-355
氨基酸序列

MPFLGQDWRS PGQSWVKTAD GWKRFLDEKS GSFVSDLSSY CNKEVYSKEN LFSSLNYDVA AKKRKKDIQN SKTKTQYFHQ EKWIYVHKGS TKERHGYCTL GEAFNRLDFS TAILDSRRFN YVVRLLELIA KSQLTSLSGI AQKNFMNILE KVVLKVLEDQ QNIRLIRELL QTLYTSLCTL VQRVGKSVLV GNINMWVYRM ETILHWQQQL NSIQISRPAF KGLTITDLPV CLQLNIMQRL SDGRDLVSLG QAAPDLHVLS EDRLLWKRLC QYHFSERQIR KRLILSDKGQ LDWKKMYFKL VRCYPRREQY GVTLQLCKHC HILSWKGTDH PCTANNPESC SVSLSPQDFI NLFKF
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點(diǎn)詳情

功能：

Substrate recognition component of a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Probably recognizes and binds to phosphorylated target proteins during skeletal muscle atrophy. Recognizes TERF1.
基因功能參考文獻(xiàn)：
1. Oligonol-mediated downregulation of Atrogin-1 and MuRF1 gene expression alleviates muscle loss and improves the impaired myotube formation, indicating that oligonol supplementation may be useful for the attenuation of myotube loss. PMID: 28930190
2. Indoxyl sulfate enhanced the production of atrogin-1 by enhancing oxidative stress in skeletal muscle, leading to muscle atrophy. PMID: 27549031
3. Valproic acid attenuated muscle wasting and myotube atrophy and reduced C/EBPbeta binding to atrogin1 promoter locus in the myotubes. PMID: 27122162
4. These results have revealed the roles for atrogin-1 in the regulation of smooth muscle contractility through enhancement of myocardin ubiquitylation/degradation and its transcriptional activity. PMID: 27403897
5. MAFbx mRNA expression was decreased in old mice relative to adult mice, whereas MuRF1 mRNA expression was less affected by ageing PMID: 26590085
6. Suggest role for atrogin-1 up-regulation in simvastatin-induced heart mitochondria dysfunction. PMID: 25300705
7. Atrogin1 was upregulated in cancer cachexia mice. Atrogin1 knockdown protected skeletal muscle cells from TNF-alpha induced atrophy. PMID: 25760630
8. Expression of USP19 correlates with that of MuRF1 and MAFbx/atrogin-1 in skeletal muscles PMID: 26048142
9. MAFbx not only regulates protein degradation, but also reduces protein synthesis, exerting a dual role in regulating cardiac mass and preventing from cardiac hypertrophy. PMID: 24650875
10. mechanical vibration strongly down-regulates atrophy genes myostatin and atrogin-1 both in vivo and in vitro. PMID: 22711460
11. mTORC1 promotes denervation-induced muscle atrophy through a mechanism involving the activation of FoxO and E3 ubiquitin ligases. PMID: 24570486
12. atrogin-1 promotes cardiomyocyte health through mediating the interplay between the ubiquitin/proteasome system and autophagy/lysosome system and its alteration promotes development of cardiomyopathies PMID: 24789905
13. Smad3 expression is sufficient to stimulate atrogin-1 promoter activity, inhibit Akt/mTOR signaling and protein synthesis, and induce muscle fiber atrophy. PMID: 24002653
14. The muscle wasting effects of the myostatin-atrogin-1 axis are not only limited to the degradation of MyoD and eukaryotic translation initiation factor 3 subunit f, but also encompass several other muscle proteins. PMID: 22673621
15. Expression of MAFbx/Atrogin-1 and MuRF1 was significantly greater in the plantaris muscle than in the soleus muscle during the early stage of atrophy. PMID: 21901639
16. C/EBPbeta-/- mice were resistant to Lewis lung cancer-induced atrogin1 upregulation and muscle wasting. Activation of the p38beta MAPK-C/EBPbeta signalling pathway appears to be a key component of cachexia. PMID: 21847090
17. Downregulation of MAFbx inhibits cardiac hypertrophy in part through stabilization of IkappaB-alpha and inactivation of nuclear factor-kappaB PMID: 21617130
18. Expression of MAFbx and Bnip3 was increased in hearts of mice in bearing colonic tumors. PMID: 21167183
19. Myogenin is upregulated in skeletal muscle following denervation and regulates expression of the E3 ubiquitin ligases MuRF1 and atrogin-1, which promote muscle proteolysis and atrophy. PMID: 20887891
20. Berberine impairs mitochonidrial function stimulating the expression of atrogin-1 without affecting phosphorylation of forkhead transcription factors. PMID: 20522589
21. atrogin-1 specifically targets truncated M7t-cMyBP-C, but not WT-cMyBP-C, for proteasomal degradation and that MuRF1 indirectly reduces cMyBP-C levels by regulating the transcription of myosin heavy chain. PMID: 19850579
22. Data show that slow in vivo release of GHRP-2 through minipump for 24h attenuated the thermal injury-induced increase in mRNA expression of IL-6 and of the E3 ubiquitin ligases, MuRF-1 and MAFbx, in rat skeletal muscle. PMID: 19577604
23. Data indicate that the SCF(atrogin-1) ubiquitin ligase complex represses calcineurin by targeting it for ubiquitin-mediated proteolysis, leading to inhibition of cardiac hypertrophy in response to pathologic stimuli. PMID: 15489953
24. MAFbx may play an important role in the course of muscle differentiation by determining the abundance of MyoD PMID: 15531760
25. activation of atrogin-1 may be a common general adaptation in cells undergoing a rapid reduction in size. PMID: 17008454
26. Results indicate that atrogin-1 does not affect the activity of Akt itself, but serves as a coactivator for members of the Forkhead family of transcription factors that function downstream of Akt. PMID: 17965779
27. atrogin-1 may be a critical mediator of the muscle damage induced by statins. PMID: 17992259
28. Targets the eukaryotic initiation factor 3 subunit 5 (eIF3-f) for ubiquitination and degradation by the proteasome. PMID: 18354498
29. Although further study is required to establish the causal relationships, downregulation of MAFbx may have reduced MyoD degradation in favor of muscle regeneration. PMID: 18785187
30. MAFbx targets MyoD for degradation in several models of skeletal muscle atrophy PMID: 19319192
31. This study demenostrated that the astrogin1 protein is reduced in skeletal muscle of chronic spinal cord-injured patients. PMID: 19533653
32. Results suggest that MAFbx functions as an F-box protein in the SCF complex for ubiquitination of myogenin. PMID: 19631210
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亞細(xì)胞定位：

Cytoplasm. Nucleus.
組織特異性：

Specifically expressed in cardiac and skeletal muscle.
數(shù)據(jù)庫鏈接：

KEGG: mmu:67731

STRING: 10090.ENSMUSP00000022986

UniGene: Mm.25683