Recombinant Mouse Electrogenic sodium bicarbonate cotransporter 1 (Slc4a4), partial

中文名稱：

小鼠Slc4a4重組蛋白
貨號：

CSB-YP021669MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Slc4a4重組蛋白
貨號：

CSB-EP021669MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Slc4a4重組蛋白
貨號：

CSB-EP021669MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Slc4a4重組蛋白
貨號：

CSB-BP021669MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Slc4a4重組蛋白
貨號：

CSB-MP021669MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Slc4a4
Uniprot No.：

O88343
別名：

Slc4a4; Nbc1; Nbce1Electrogenic sodium bicarbonate cotransporter 1; Sodium bicarbonate cotransporter; Na(+)/HCO3(-) cotransporter; Solute carrier family 4 member 4
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Electrogenic sodium/bicarbonate cotransporter with a Na(+):HCO3(-) stoichiometry varying from 1:2 to 1:3. May regulate bicarbonate influx/efflux at the basolateral membrane of cells and regulate intracellular pH.; May have a higher activity than isoform 1.
基因功能參考文獻：
1. The data of this study showed for the first time that NBCe1 is a direct target of TGF-beta/Smad4 signaling. PMID: 28568893
2. NBCe1 is necessary for normal proximal tubule NaDC-1 expression and 2-oxoglutarate metabolism, and deletion results in stimulation of a novel citrate reabsorptive pathway PMID: 27117802
3. Reversed electrogenic sodium bicarbonate cotransporter 1 is the major acid loader during recovery from cytosolic alkalosis in mouse cortical astrocytes PMID: 25990710
4. Study presented evidence for mouse cortical astrocytes and Xenopus oocytes that total "effective" cytosolic H+ buffering does not only include instantaneous, physicochemical buffering, but also rapid bicarbonate transport via NBCe1 PMID: 25820238
5. NBCe1 deletion caused a gene dose-related decrease in ammonia excretion PMID: 26224717
6. Results demonstrate that transcriptional regulation and targeting of NBCe1, as well as functional operation of NBCe1, may occur through multiple signaling pathways PMID: 25755028
7. The expression of NBCe1 in ameloblasts and the papillary layer cell depends on the developmental stage and possibly responds to pH changes. PMID: 25012520
8. NBCe1 function is regulated by protein kinase C in murine colonic crypts. PMID: 24642792
9. Our results demonstrate a surprisingly high apparent bicarbonate sensitivity mediated by NBCe1 in cortical astrocytes PMID: 24453308
10. acidosis-induced NBCe1-A, but not NBCe1-B/C, trafficking is mediated by Rab8a. PMID: 23583738
11. Cloning of NBCe1-D and NBCe1-E, both lacking a 27-nucleotide cassette I, reveals a novel alternative splicing unit in the mouse Slc4a4 gene. PMID: 21600280
12. Created NBC1 W516X knock-in mice as a model of human isolated proximal renal tubular acidosis. PMID: 21228764
13. NBCe1 expression is required for the development of normal enamel PMID: 20529845
14. existence of an intracellular HCO(3)(-) concentration- and cell volume-independent activation of colonic NBC by an increase in intracellular cAMP. PMID: 12388213
15. These results suggest that the pancreatic sodium-bicarbonate cotransporter contributes to pH(i) regulation in the parotid acinar cells. PMID: 12727194
16. regulation through IRBIT enables NBC1 variants to have different physiological roles PMID: 16769890
17. NBC1 activity is a component of the basolateral mechanisms for HCO3- uptake during cAMP-stimulated anion secretion in the proximal colon PMID: 17192275
18. these data demonstrate distinct cellular distribution of Slc4a4 NH2-terminal variants in mouse brain PMID: 17433553
19. It is likely that NBC1, 4.1B, and p55 form a molecular complex in the basolateral membrane of the kidney S1-S2 proximal tubules. PMID: 17712176
20. NBCe1 is expressed in ameloblasts in vivo in a polarized fashion, thereby providing a mechanism for ameloblast transcellular bicarbonate secretion in the process of enamel formation and maturation PMID: 18362326
21. wt-NBCe1-A is predominantly a homodimer, dependent on S-S bond formation that is composed of functionally active monomers. PMID: 18658147
22. Data show that pNBC1 activation requires the IRBIT PEST domain, while CFTR activation requires multiple domains, and suggest that IRBIT is a key coordinator of epithelial fluid and HCO3- secretion. PMID: 19033647
23. Secretagogue stimulation enhances NBCe1 (electrogenic Na(+)/HCO(3)(-) cotransporter) surface expression in murine colonic crypts. PMID: 19779011
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亞細胞定位：

Basolateral cell membrane; Multi-pass membrane protein.
蛋白家族：

Anion exchanger (TC 2.A.31) family
組織特異性：

Isoform 1 is specifically expressed in pancreatic ducts and acini. Also expressed in parotid acinar cells and in the colonic crypts.
數據庫鏈接：

KEGG: mmu:54403

STRING: 10090.ENSMUSP00000121744

UniGene: Mm.41044