Recombinant Mouse D-amino-acid oxidase (Dao)

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品名簡稱：

Recombinant Mouse Dao protein
貨號：

CSB-EP006494MO
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Dao
Uniprot No.：

P18894
別名：

(DAAO)(DAMOX)(DAO)
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

42.7 kDa
表達區域：

1-345aa
氨基酸序列

MRVAVIGAGVIGLSTALCIHERYHPTQPLHMKIYADRFTPFTTSDVAAGLWQPYLSDPSNPQEAEWSQQTFDYLLSCLHSPNAEKMGLALISGYNLFRDEVPDPFWKNAVLGFRKLTPSEMDLFPDYGYGWFNTSLLLEGKSYLPWLTERLTERGVKLIHRKVESLEEVARGVDVIINCTGVWAGALQADASLQPGRGQIIQVEAPWIKHFILTHDPSLGIYNSPYIIPGSKTVTLGGIFQLGNWSGLNSVRDHNTIWKSCCKLEPTLKNARIVGELTGFRPVRPQVRLEREWLRHGSSSAEVIHNYGHGGYGLTIHWGCAMEAANLFGKILEEKKLSRLPPSHL
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Regulates the level of the neuromodulator D-serine in the brain. Has high activity towards D-DOPA and contributes to dopamine synthesis. Could act as a detoxifying agent which removes D-amino acids accumulated during aging. Acts on a variety of D-amino acids with a preference for those having small hydrophobic side chains followed by those bearing polar, aromatic, and basic groups. Does not act on acidic amino acids.
基因功能參考文獻：
1. This work provides the most complete baseline analysis of L- and D-amino acids in the brains of wild-type and mutant mice lacking D-amino acid oxidase activity. PMID: 29532193
2. findings reveal that DAO is a microbiota-regulated host innate immune factor that modulates growth of both pathogens and commensals, primarily in the small intestine PMID: 27670111
3. some significant in vivo effects of DAOR199W on motor neuron function (i.e. kyphosis and loss of motor neurons) were detected which were most marked in females and could contribute to the earlier onset of neurological signs in double transgenic females compared to SOD1G93A littermates, highlighting the importance of recognizing gender effects present in animal models of ALS PMID: 29194436
4. Long-term spatial memory is unaltered in Dao-/- mice. PMID: 26833794
5. Dao-/- mice demonstrated enhanced spatial recognition memory and no sleep or circadian rhythm disruption. PMID: 25816902
6. In d-Amino acid oxidase (-/-) mice, approximately twice as many Dopamine-like neurons fired in a bursting pattern than in DAO(+/-) or DAO(+/+) mice. Data provide the first direct evidence that DAO modulates VTA DA neuron activity PMID: 25040393
7. The retinal levels of d-serine are tightly controlled during the first week of postnatal life and vary according to deficiencies in either DAO/ PMID: 25083578
8. DAO deficiency reduces D-serine level in retina and increases NMDA receptor expression. PMID: 24068757
9. D-amino acids and DAO have pivotal neurotransmission functions in the central nervous system. (Review) PMID: 22892863
10. D-Amino acid oxidase-induced oxidative stress, 3-bromopyruvate and citrate inhibit angiogenesis, exhibiting potent anticancer effects. PMID: 22802136
11. results suggest that DAO, together with myeloperoxidase, may play an important role in antibacterial systems in mammals PMID: 22271930
12. DAO has a pivotal role in motoneuron degeneration through D-serine regulation, with inactivity of DAO being a common feature between the mSOD1 ALS mouse model and mutant DAO-associated familial ALS. PMID: 22203986
13. Results confirm the role of D-amino acid oxidase (DAO) in D-serine metabolism, and provide a tool to investigate DAO, an enzyme currently of considerable interest in the pathophysiology and therapy of schizophrenia. PMID: 20828614
14. data show enhanced PPI responses and enhanced PPI-disruptive effects of NMDA antagonists in DAO-/- mice . PMID: 20933022
15. Familial amyotrophic lateral sclerosis is associated with a mutation in D-amino acid oxidase PMID: 20368421
16. Hypofunctional Dao 1 mutation elevated brain levels of D-serine. Mice with both Dao1 and Grin 1 mutations had improved social approach spatial memory retention, reversal of latent inhibition, and partial normalized startle responses. PMID: 19751394
17. These results suggest that the abundant D-amino-acids in the mutant mouse brain facilitate hippocampal LTP and spatial learning. PMID: 15996778
18. Lacks DAO activity and shown for the first time it has increased occupancy of the NMDAR glycine site due to elevated extracellular D-serine levels and has enhanced NMDAR function in vivo. PMID: 16843004
19. Its physiological role is in metabolizing endogenous and exogenous D-amino acids. PMID: 18516913
20. D-amino acid oxidase may be a promising therapeutic target to improve cognitive flexibility and inhibitory learning in psychiatric disorders such as schizophrenia and anxiety syndromes. PMID: 19117914
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亞細胞定位：

Peroxisome.
蛋白家族：

DAMOX/DASOX family
數據庫鏈接：

KEGG: mmu:13142

STRING: 10090.ENSMUSP00000107911

UniGene: Mm.20115