Recombinant Mouse Cysteine protease ATG4B (Atg4b)

中文名稱：

Recombinant Mouse Cysteine protease ATG4B(Atg4b)
貨號：

CSB-YP807332MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

Recombinant Mouse Cysteine protease ATG4B(Atg4b)
貨號：

CSB-EP807332MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Mouse Cysteine protease ATG4B(Atg4b)
貨號：

CSB-BP807332MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Mouse Cysteine protease ATG4B(Atg4b)
貨號：

CSB-MP807332MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Atg4b
Uniprot No.：

Q8BGE6
別名：

Atg4b; Apg4b; Autl1; Kiaa0943Cysteine protease ATG4B; EC 3.4.22.-; AUT-like 1 cysteine endopeptidase; Autophagin-1; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區域：

1-393
氨基酸序列

MDAATLTYDT LRFAEFEDFP ETSEPVWILG RKYSIFTEKD EILSDVASRL WFTYRRNFPA IGGTGPTSDT GWGCMLRCGQ MIFAQALVCR HLGRDWRWTQ RKRQPDSYFN VLNAFLDRKD SYYSIHQIAQ MGVGEGKSIG QWYGPNTVAQ VLKKLAVFDT WSSLAVHIAM DNTVVMEEIR RLCRANLPCA GAAALPTDSE RHCNGFPAGA EVTNRPSAWR PLVLLIPLRL GLTDINEAYV ETLKHCFMMP QSLGVIGGKP NSAHYFIGYV GEELIYLDPH TTQPAVELTD SCFIPDESFH CQHPPSRMGI GELDPSIAVG FFCKTEEDFN DWCQQVKKLS QLGGALPMFE LVEQQPSHLA CQDVLNLSLD SSDVERLERF FDSEDEDFEI LSL
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.
基因功能參考文獻：
1. At the microtubule-actin crossover junction, ATG4B detaches LC3B from melanosomal membranes by enzymatic delipidation. PMID: 28598240
2. ATG4B contains a C-terminal LIR motif important for binding and efficient cleavage of mammalian orthologs of yeast Atg8 PMID: 28287329
3. miR-34a might regulate the autophagic activity and can cause injury in ischemia reperfusion injured renal tubular epithelial cells (RTEC) via targeting Atg4B. Atg4B knockdown inhibited autophagy in RTECs. PMID: 26406385
4. the ATG4B protease and autophagy play a crucial role protecting epithelial cells against bleomycin-induced stress and apoptosis PMID: 25906080
5. REDD1/TXNIP complex expression is sufficient to induce reactive oxygen species, suppress ATG4B activity and activate autophagy. PMID: 25916556
6. these findings demonstrate a role for Ser-383 and Ser-392 phosphorylation of ATG4B in control of autophagy. PMID: 26378241
7. mTOR inhibition alleviates Huntington's disease progression by inducing Atg4b-dependent autophagic flux. PMID: 23861892
8. An autophagy-related gene, Atg4b, is identified as a de novo target gene of C/EBP BETA and is shown to play an important role in 3T3-L1 adipocyte differentiation. PMID: 23754749
9. The Atg4b participates in polarized secretion of lysosomal contents into the extracellular space by directing lysosomes to fuse with the plasma membrane. PMID: 22055344
10. Genetic inactivation of mouse Atg4b resulted in amorphous globular bodies in the neuropil of the deep cerebellar nuclei and adjacent vestibular nuclei and a mild but measurable impairment of motor performance on the Rotarod. PMID: 20634410
11. The Apg4B/autophagin-1 protease thus serves as a processing/deconjugating enzyme for these four highly divergent mammalian Apg8 homologues, GATE-16, MAP1-LC3, GABARAP, and Apg8L. PMID: 14530254
12. These results show that the lipidation of LC3 paralogues is involved in the completion of autophagosome formation in mammalian cells. PMID: 18768752
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亞細胞定位：

Cytoplasm.
蛋白家族：

Peptidase C54 family
數據庫鏈接：

KEGG: mmu:66615

STRING: 10090.ENSMUSP00000027502

UniGene: Mm.29087