Recombinant Mouse Cyclic AMP-dependent transcription factor ATF-4 (Atf4)

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中文名稱：

Recombinant Mouse Cyclic AMP-dependent transcription factor ATF-4 (Atf4)
品名簡稱：

Recombinant Mouse Atf4 protein
貨號：

CSB-EP002272MOb0
說明書：

MSDS Datasheet
規格：

￥1536
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Atf4
Uniprot No.：

Q06507
別名：

cAMP-dependent transcription factor ATF-4;Activating transcription factor 4;C/EBP-related ATF;C/ATF;Cyclic AMP-responsive element-binding protein 2;CREB-2;cAMP-responsive element-binding protein 2
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

44.4 kDa
表達區域：

1-349aa
氨基酸序列

MTEMSFLNSEVLAGDLMSPFDQSGLGAEESLGLLDDYLEVAKHLKPHGFSSDKAGSSEWPAMDDGLASASDTGKEDAFSGTDWMLEKMDLKEFDFDALFRMDDLETMPDELLTTLDDTCDLFAPLVQETNKEPPQTVNPIGHLPESLIKVDQVAPFTFLQPFPCSPGVLSSTPEHSFSLELGSEVDISEGDRKPDSAAYITLIPPCVKEEDTPSDNDSGICMSPESYLGSPQHSPSTSRAPPDNLPSPGGSRGSPRPKPYDPPGVSLTAKVKTEKLDKKLKKMEQNKTAATRYRQKKRAEQEALTGECKELEKKNEALKEKADSLAKEIQYLKDLIEEVRKARGKKRVP
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

Transcription factor that binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3') and displays two biological functions, as regulator of metabolic and redox processes under normal cellular conditions, and as master transcription factor during integrated stress response (ISR). Binds to asymmetric CRE's as a heterodimer and to palindromic CRE's as a homodimer. Core effector of the ISR, which is required for adaptation to various stress such as endoplasmic reticulum (ER) stress, amino acid starvation, mitochondrial stress or oxidative stress. During ISR, ATF4 translation is induced via an alternative ribosome translation re-initiation mechanism in response to EIF2S1/eIF-2-alpha phosphorylation, and stress-induced ATF4 acts as a master transcription factor of stress-responsive genes in order to promote cell recovery. Promotes the transcription of genes linked to amino acid sufficiency and resistance to oxidative stress to protect cells against metabolic consequences of ER oxidation. Activates the transcription of NLRP1, possibly in concert with other factors in response to ER stress. Activates the transcription of asparagine synthetase (ASNS) in response to amino acid deprivation or ER stress. However, when associated with DDIT3/CHOP, the transcriptional activation of the ASNS gene is inhibited in response to amino acid deprivation. Together with DDIT3/CHOP, mediates programmed cell death by promoting the expression of genes involved in cellular amino acid metabolic processes, mRNA translation and the terminal unfolded protein response (terminal UPR), a cellular response that elicits programmed cell death when ER stress is prolonged and unresolved. Together with DDIT3/CHOP, activates the transcription of the IRS-regulator TRIB3 and promotes ER stress-induced neuronal cell death by regulating the expression of BBC3/PUMA in response to ER stress. May cooperate with the UPR transcriptional regulator QRICH1 to regulate ER protein homeostasis which is critical for cell viability in response to ER stress. In the absence of stress, ATF4 translation is at low levels and it is required for normal metabolic processes such as embryonic lens formation, fetal liver hematopoiesis, bone development and synaptic plasticity. Acts as a regulator of osteoblast differentiation in response to phosphorylation by RPS6KA3/RSK2: phosphorylation in osteoblasts enhances transactivation activity and promotes expression of osteoblast-specific genes and post-transcriptionally regulates the synthesis of Type I collagen, the main constituent of the bone matrix. Cooperates with FOXO1 in osteoblasts to regulate glucose homeostasis through suppression of beta-cell production and decrease in insulin production. Activates transcription of SIRT4. Regulates the circadian expression of the core clock component PER2 and the serotonin transporter SLC6A4. Binds in a circadian time-dependent manner to the cAMP response elements (CRE) in the SLC6A4 and PER2 promoters and periodically activates the transcription of these genes. Mainly acts as a transcriptional activator in cellular stress adaptation, but it can also act as a transcriptional repressor: acts as a regulator of synaptic plasticity by repressing transcription, thereby inhibiting induction and maintenance of long-term memory. Regulates synaptic functions via interaction with DISC1 in neurons, which inhibits ATF4 transcription factor activity by disrupting ATF4 dimerization and DNA-binding.
基因功能參考文獻：
1. Data suggest that endoplasmic reticulum stress-induced CHOP/Ddit3 inhibits expression of Bip/Grp78 and Atf4; ATF4, in turn, plays critical role in CHOP-mediated regulation of B-cell receptor-controlled murine gammaherpesvirus-68 lytic replication. (CHOP/Ddit3 = DNA-damage inducible transcript-3; Bip/Grp78 = chaperone BiP 78 kDa; Atf4 = activating transcription factor-4) PMID: 29305424
2. The results suggest that ATF4 may serve a protective role in the mouse liver. PMID: 29845243
3. Golgi stress response elicited by monensin stimulates CSE by acting via ATF4 with characteristics distinguishable from the endoplasmic reticulum stress response PMID: 29317536
4. established Neuro2a cells with edited GADD34 and ATF4/GADD34 genes and found that ATF4 acts as a proapoptotic factor, but GADD34 depletion did not attenuate the expression of cleaved caspase-3 induced by tunicamycin treatment. PMID: 28825160
5. Atg7 ablation mainly induced the PERK-ATF4-CHOP axis of the endoplasmic reticulum (ER) stress response in growth plate chondrocytes. PMID: 28304100
6. under nutrient-limiting conditions that stimulate ATF4 activity, TRIB3 is implicated in the regulation of metabolic adaptation by restraining the transcription of Fgf21. PMID: 29378327
7. these findings reveal a new crucial combined effect of the silencing of PERK and ATF4 in modulating ER stressmediated apoptosis during chondrocyte differentiation and proliferation. PMID: 28498443
8. Sirt1 reduced endoplasmic reticulum stress and apoptosis of brown adipocytes in vivo/in vitro by inhibiting Smad3/ATF4 signaling pathway. PMID: 28030827
9. These findings indicate that the aggregation of S-opsin induced by exposure to blue -emitting diode light causes endoplasmic reticulum stress, and ATF4 activation in particular. PMID: 28331281
10. We hypothesize that the essential role of methionine-charged initiator tRNA in forming ternary complex is responsible for the robust ability of methionine deficiency to induce ATF4 and the ISR even in the absence of GCN2 or eIF2alpha kinase activity. PMID: 27613409
11. BTG1 has a role in regulating hepatic lipid metabolism and in preventing ATF4 and SCD1 from inducing liver steatosis PMID: 27188441
12. Transcriptional profiling reveals that mouse neuroblastoma sphere-forming cells acquire a metabolic program characterized by transcriptional activation of the cholesterol and serine-glycine synthesis pathways, primarily as a result of increased expression of sterol regulatory element binding factors and Atf4, respectively PMID: 27705805
13. MIF-2/D-DT is an early response cytokine in the I/R injury repair of the proximal tubule, enhancing regeneration through SLPI- and ATF4-dependent mechanisms. PMID: 28539339
14. ATF4 has a role in gene expression during basal conditions, with 385 genes altered by the loss of ATF4 in the absence of apparent stress. Deletion of ATF4 alters genes that are required for the conversion of cholesterol to bile acid (CYP7A1), esterification of cholesterol (SOAT2), and transport from the hepatocyte (ABCA1); when ATF4 loss is coupled with ER stress, results in increase in free cholesterol within hepatocyte PMID: 26960794
15. ATF4 pathway is activated in vivo upon mitochondrial stress. PMID: 28566324
16. Data, including data from studies using cells from knockout mice, suggest that gasotransmitter H(2)S up-regulates eIF2a phosphorylation by inhibiting PPP1CA via persulfidation, which in turn leads to transient suppression of global translation and activation of Atf4 expression. (eIF2a = eukaryotic initiation factor-2alpha; PPP1CA = protein phosphatase 1 catalytic subunit alpha; Atf4 = activating transcription factor 4) PMID: 28637872
17. Results identify the beneficial role of hypothalamic ATF4/ATG5 axis in the regulation of energy expenditure, obesity, and obesity-related metabolic disorders. PMID: 28213613
18. SLC30A10 has a protective role in 1-methyl-4-phenylpyridinium-induced toxicity via PERK-ATF4 pathway. PMID: 28688763
19. ER stress-induced increase in ATF4 and CHOP expression is initiated by an increase in Atf4 and Chop mRNA, which is also dependent upon eIF2alpha phosphorylation. PMID: 28478109
20. Augmented ATF4 signals during retinal degeneration plays a cytotoxic role by triggering photoreceptor cell death. PMID: 27144303
21. ATF4 might inhibit the transcription of Srebp1c through TRB3, which is repressed by IBMX and DEX during early adipogenesis PMID: 27452504
22. ATF4 activates a negative-feedback loop, leading to the downregulation of RET expression while upregulating expression of proapoptotic genes. PMID: 27935748
23. Data demonstrate a novel function of ATF4 in agouti-related peptide neurons of the hypothalamus in energy balance and lipid metabolism. PMID: 27993927
24. The role of dietary zinc in the expression of proteins involved in the endoplasmic reticulum stress response is reported. PMID: 27605406
25. these results identify the ATF4-TRB3-AMPK axis as a novel pathway responsible for ethanol-induced liver steatosis. PMID: 27405764
26. arsenic trioxide -mediated apoptosis is regulated by both endoplasmic reticulum and mitochondria events that are facilitated by ATF4 and the unfolded protein response PMID: 27638049
27. Findings indicate that PERK kinase-activating transcription factor 4 (ATF4) pathway affected the efficiency of X-box binding protein 1 (XBP1) mRNA splicing by regulating inositol-requiring enzyme 1alpha (IRE1alpha) expression. PMID: 27052593
28. Interference with DOT1L activity resulted in transcriptional activation of Atf4 and Ddit3 accompanied by decreased levels of H3K79 dimethylation. PMID: 26299268
29. results suggest that by inducing an atypical ISR and p53-independent apoptosis, ONC201 has clinical potential in hematological malignancies. PMID: 26884599
30. TFEB-regulated signaling pathway for osteoblast differentiation is involved in ATF4/CHOP-dependent signaling pathway. PMID: 26519689
31. These findings identify C/EBPgamma as a novel antioxidant regulator and an obligatory ATF4 partner that controls redox homeostasis in normal and cancerous cells. PMID: 26667036
32. Gcn2 & Atf4 are involved in L-proline metabolism regulation in embryonic stem cells. PMID: 25857264
33. This study outlines the mechanism of NIR laser phototoxicity and the utility of monitoring surface temperature and ATF4 expression as potential biomarkers to develop safe and effective clinical applications. PMID: 26030745
34. ATF4 plays a pivotal role in functional expansion and repopulating efficiency of HSCs in developing FL PMID: 26384355
35. results identify ursolic acid and tomatidine as potential agents and/or lead compounds for reducing ATF4 activity, weakness, and atrophy in aged skeletal muscle PMID: 26338703
36. The CARE-LUC mouse model represents an innovative tool to investigate the eIF2alpha-ATF4 axis and to develop drugs targeting this important pathway in the remediation of related pathologies. PMID: 25921292
37. exogenous overexpression of ATF4 in breast cancer cells may facilitate the recruitment of macrophages into tumor tissues and promote tumor angiogenesis and tumor growth indirectly. PMID: 25883982
38. We conclude that ATF4 is a key regulator of the physiological state necessary for neuronal plasticity and memory. PMID: 25865882
39. Elevation of ATF4, at least in liver, thus seems to be a shared feature of diets, drugs, genes, and developmental alterations that extend maximum lifespan in mice. PMID: 25156122
40. ATF4 signaling pathway is essential for mediating the effect of ER stress on beta-klotho expression. PMID: 25727012
41. The miR-214-ATF4 axis is a novel pathway for the regulation of hepatic gluconeogenesis. PMID: 25657009
42. Target genes of ATF4 activity are not only important to osteoblast differentiation but also in maintaining bone toughness and fracture toughness. PMID: 24509412
43. The minimum promoter region required for Mc3r expression has been identified, along with two binding sites for AP-1 and ATF4 and in the 5' upstream-flanking region of Mc3r that are essential for Mc3r expression. PMID: 25701401
44. Fibroblasts from two long-lived mice mutants (Snell dwarf and PAPP-A knockout) were found to have higher levels of ATF4 protein expression suggesting a connection to longevity. PMID: 24691093
45. FGF21 is the target gene for activating transcription factor 4. PMID: 24900988
46. BMP-2 stimulates differentiation of myoblasts into osteoblasts via the PERK-eIF2alpha-ATF4 pathway but in addition stimulates Tmem119, which itself increases ATF4 PMID: 24362451
47. Data (from studies in transgenic/knockout mice) suggest p21/Cdkn1a (cyclin-dependent kinase inhibitor 1A) expression is up-regulated in immobilized, atrophied skeletal muscle via combined actions of Atf4 and p53 (transformation related protein 53). PMID: 24895282
48. C/EBPbeta and peroxisome proliferator-activated receptor gamma (but not tribbles homolog 3) are targeted for activation by activating ATF4, a member of cAMP response element-binding/activator transcription factor family. PMID: 24673832
49. ATF4 expression in the liver is responsible for the protective effects against high fat diet-induced CYP2E1 expression. PMID: 24373582
50. the loss of function of either DISC1 or ATF4 increases PDE4D9 transcription, and the association of DISC1 with the PDE4D9 locus requires ATF4 PMID: 23587879
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亞細胞定位：

Nucleus. Nucleus speckle. Cytoplasm. Cell membrane. Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.
蛋白家族：

BZIP family
組織特異性：

Ubiquitously expressed in adults.
數據庫鏈接：

KEGG: mmu:11911

STRING: 10090.ENSMUSP00000105234

UniGene: Mm.641