Recombinant Mouse Calsequestrin-2 (Casq2)

中文名稱：

Recombinant Mouse Calsequestrin-2(Casq2)
貨號(hào)：

CSB-YP004557MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Mouse Calsequestrin-2(Casq2)
貨號(hào)：

CSB-EP004557MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Mouse Calsequestrin-2(Casq2)
貨號(hào)：

CSB-EP004557MO-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Mouse Calsequestrin-2(Casq2)
貨號(hào)：

CSB-BP004557MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Mouse Calsequestrin-2(Casq2)
貨號(hào)：

CSB-MP004557MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Casq2
Uniprot No.：

O09161
別名：

Casq2Calsequestrin-2; Calsequestrin; cardiac muscle isoform
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Full Length of Mature Protein
表達(dá)區(qū)域：

20-415
氨基酸序列

E EGLNFPTYDG KDRVVSLSEK NLKQMLKRYD LLCLYYHEPV SSDKVSQKQF QLKEIVLELV AQVLEHKNIG FVMVDSRKEA KLAKRLGFSE EGSLYVLKGD RTIEFDGEFA ADVLVEFLLD LIEDPVEIVN NKLEVQAFER IEDQTKLLGF FKNEDSEYYK AFQEAAEHFQ PYIKFFATFD KAVAKKLSLK MNEVGFYEPF MDEPNVIPNK PYTEEELVEF VKEHQRPTLR RLRPEDMFET WEDDLNGIHI VAFAEKSDPD GYEFLEILKQ VARDNTDNPD LSILWIDPDD FPLLVAYWEK TFKIDLFKPQ IGVVNVTDAD SIWMEIPDDD DLPTAEELED WIEDVLSGKI NTEDDDNEDE DDDGDDNDDD DDDDDDNDNS DEDNEDSDDD DDDDE
蛋白標(biāo)簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. Calcium ions are bound by clusters of acidic residues at the protein surface, especially at the interface between subunits. Can bind around 60 Ca(2+) ions. Regulates the release of lumenal Ca(2+) via the calcium release channel RYR2; this plays an important role in triggering muscle contraction. Plays a role in excitation-contraction coupling in the heart and in regulating the rate of heart beats.
基因功能參考文獻(xiàn)：
1. CASQ2-/- causes increase in calsequestrin 1 (CASQ1) expression. PMID: 28130614
2. These data suggest a functional role of CSQ2 not only in the ventricle but also in the atrium of mammalian hearts PMID: 27484853
3. The minimal KEKE motif of TRN involved in the interactions with CSQ2, HRC and RyR2 within the Ca2+ release units of cardiac sarcoplasmic reticulum has been defined. PMID: 26674963
4. While CASQ2 stabilizes RyR2 rendering it refractory in the diastolic phase, HRC enhances RyR2 activity facilitating RyR2 recovery from refractoriness. PMID: 26410369
5. Results show that CASQ2 deletion causes abnormal sarcoplasmic reticulum Ca(2+) release and selective interstitial fibrosis in the atrial pacemaker complex, which disrupt SAN pacemaking and increase susceptibility to atrial fibrillation PMID: 24216388
6. Viral gene transfer of wild-type CASQ2 into the heart of R33Q mice prevents and reverts severe manifestations of catecholaminergic polymorphic ventricular tachycardia. PMID: 24888331
7. Cardiac hypertrophy and calcium waves in CASQ2-null cardiac muscle are governed by the ryanodine receptor (Ryr)2 Ca2+ sensor. PMID: 24758151
8. Expression of CASQ2-R33Q influences molecular and ultra-structural heart development; post-natal, adaptive changes appear capable of ensuring until adulthood a new pathophysiological equilibrium. PMID: 24370574
9. Mutant CASQ2(D307H) protein retains some of its physiological function. Its expression decreases with age and is inversely related to arrhythmia severity. PMID: 24070655
10. Qualitatively similar results were obtained in a hybrid strain created by crossing CASQ2 knockout mice with mice deficient in phospholamban. PMID: 23135969
11. Aberrant disordered calcium release events can become synchronized in the intact myocardium, leading to triggered activity and the resultant diastolic contractions in the settings of a cardiac rhythm disorder. PMID: 23733959
12. The triadin-to-calsequestrin ratio is a critical modulator of the sarcoplasmic reticulum Ca(2+) signaling in ventricular myocytes. PMID: 22427521
13. The results presented in this paper support the idea of Casq2 acting both as a buffer and a direct regulator of the Ca(2+) release process. PMID: 21983287
14. Ca(2+) and JNT-dependent disassembly of the CSQ2 polymer PMID: 22123818
15. Suggest that CASQ2(D307H) point mutation may affect Ca(2+) buffering capacity and Ca(2+) release in cardiac myocytes. PMID: 21984545
16. Report prevention of ventricular arrhythmia and calcium dysregulation in a catecholaminergic polymorphic ventricular tachycardia mouse model carrying calsequestrin-2 mutation. PMID: 20807279
17. calsequestrin mutation CASQ2D307H does not affect protein stability and targeting to the junctional sarcoplasmic reticulum but compromises its dynamic regulation of calcium buffering PMID: 19920148
18. gene structure, developmental and tissue specific expression of the murine, cardiac isoform of calsequestrin. PMID: 11770083
19. Lack of Casq2 causes increased diastolic sarcoplasmic reticulum Ca2+ leak, rendering Casq2-null mice susceptible to catecholaminergic ventricular arrhythmias. PMID: 16932808
20. central role of RyR2 dysfunction in CASQ2 deficiency unifies the pathophysiologic mechanism underlying Catecholamine-induced polymorphic ventricular tachycardia due to RyR2 or CASQ2 mutations PMID: 17607358
21. Reductions in Casq2 increase sarcoplasmic recticulum Ca2+ leak and cause ventricular tachycardia susceptibility under stress. Casq2 likely regulates sarcoplasmic reticulum calcium channels. PMID: 17656677
22. abnormalities in the CASQ2(R33Q/R33Q) CPVT mouse model include (1) reduced CASQ2 content, (2) reduction of SR calcium content, (3) dilatation of junctional SR, and (4) impaired clustering of mutant CASQ2. PMID: 18583715
23. REVIEW of Casq2 mutations that cause catecholaminergic polymorphic ventricular tachycardia and their effects on Casq2 function and Ca handling PMID: 18669926
24. Egr-1 negatively regulates calsequestrin expression and calcium dynamics in ventricular cells. PMID: 19103607
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亞細(xì)胞定位：

Sarcoplasmic reticulum lumen.
蛋白家族：

Calsequestrin family
組織特異性：

Detected in heart (at protein level). Detected in heart.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:12373

STRING: 10090.ENSMUSP00000029454

UniGene: Mm.15343