Recombinant Mouse Alpha-fetoprotein (Afp)

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中文名稱(chēng)：

Recombinant Mouse Alpha-fetoprotein(Afp)
品名簡(jiǎn)稱(chēng)：

Recombinant Mouse Afp protein
貨號(hào)：

CSB-EP001421MO
說(shuō)明書(shū)：

MSDS Datasheet
規(guī)格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 90% as determined by SDS-PAGE.
基因名：

Afp
Uniprot No.：

P02772
別名：

AfpAlpha-fetoprotein; Alpha-1-fetoprotein; Alpha-fetoglobulin
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Full Length of Mature Protein
來(lái)源：

E.coli
分子量：

69.3kDa
表達(dá)區(qū)域：

19-605aa
氨基酸序列

KALHENEFGIASTLDSSQCVTEKNVLSIATITFTQFVPEATEEEVNKMTSDVLAAMKKNSGDGCLESQLSVFLDEICHETELSNKYGLSGCCSQSGVERHQCLLARKKTAPASVPPFQFPEPAESCKAHEENRAVFMNRFIYEVSRRNPFMYAPAILSLAAQYDKVVLACCKADNKEECFQTKRASIAKELREGSMLNEHVCSVIRKFGSRNLQATTIIKLSQKLTEANFTEIQKLALDVAHIHEECCQGNSLECLQDGEKVMTYICSQQNILSSKIAECCKLPMIQLGFCIIHAENGVKPEGLSLNPSQFLGDRNFAQFSSEEKIMFMASFLHEYSRTHPNLPVSVILRIAKTYQEILEKCSQSGNLPGCQDNLEEELQKHIEESQALSKQSCALYQTLGDYKLQNLFLIGYTRKAPQLTSAELIDLTGKMVSIASTCCQLSEEKWSGCGEGMADIFIGHLCIRNEASPVNSGISHCCNSSYSNRRLCITSFLRDETYAPPPFSEDKFIFHKDLCQAQGKALQTMKQELLINLVKQKPELTEEQLAAVTADFSGLLEKCCKAQDQEVCFTEEGPKLISKTRDALGV
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol.
Note: If you have any special requirement for the glycerol content, please remark when you place the order.
If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產(chǎn)品描述：

This Recombinant Mouse Afp protein was made through genetic engineering. By putting the Afp gene into the genetic material of E.coli cell, the E.coli could be used as factories or producers to make the desired Afp protein for research uses. The expression region of this protein is at 19-605aa. N-terminal 6xHis tag was used in the expression process. The purity is 90%+ determined by SDS-PAGE.
Alpha-fetoprotein is a kind of glycoprotein, derived from embryonic endoderm tissue cells. AFP content in fetal serum is high and gradually decreases to the level of adults after birth. The low content of AFP in the adult blood is mainly due to the loss of the ability to synthesize AFP in mature hepatocytes. When transformed, the liver cancer cells can regain the ability to synthesize AFP. Besides liver cancer, malignant tumors from stomach, pancreas, and reproductive system are often accompanied by a small amount of increased AFP. In general, AFP is produced only at low levels after birth. Combining detection of AFP with new diagnostic markers increases the sensitivity and specificity to diagnose HCC. For instance, combination of AFP and lens culinaris lectin detection resulted in increased specificity and sensitivity in diagnosing HCC. With the progress of clinical laboratory techniques, the new liver biomarkers have displayed important roles in the diagnosis of liver cancer. Studies have dissected the immunodominant epitopes of AFP in order to effectively use this protein for immunotherapy of AFP expressing tumors. AFP could be a promising candidate for immunotherapy. In this way, we may overcome the immune tolerance of AFP and eliminate the cancer cells.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Binds estrogens, fatty acids and metals.
基因功能參考文獻(xiàn)：
1. endogenous AFP expression in the perinatal liver is reduced in the absence of beta-catenin, suggesting that beta-catenin regulates AFP during liver development. PMID: 22544812
2. Findings indicate that AFP expressed under experimental cryptorchid conditions plays a role as a regulatory factor in spermatogenesis and in hepatic generation. PMID: 21573244
3. AFP mRNA and VEGF-C mRNA in peripheral blood were significantly correlated with recurrence and metastasis of hepatocellular carcinoma. PMID: 20506647
4. A DEHP treatment-induced phenotype was identified with lack of glycogen accumula-tion and intracytoplasmic localization of beta-catenin which was associated with increased AFP gene expression. PMID: 20307648
5. The deficits observed in parental behavior of AFP knockout females could not be explained by any changes in olfactory function, novelty recognition or anxiety PMID: 20109458
6. Foxa1 acts as a pioneer transcription factor in de novo activation of Afp, by exploiting a lack of methylation at juxtaposed transposed elements PMID: 20348100
7. The level of AFP mRNA in peripheral blood may indicate recurrence and/or metastasis after curative resection of hepatocellular carcinoma. PMID: 12113675
8. AFP affects cell proliferation by binding its receptor to trigger the signal transduction pathway of cAMP-PKA and alter the expression of K- ras p21 genes PMID: 12118941
9. A murine Nkx2.8 was isolated from the Hepal-6 cell line and showed oligonucleotide binding competitive with fetoprotein transcription factor. PMID: 12167706
10. The mouse alpha-fetoprotein promoter is repressed in HepG2 hepatoma cells by hepatocyte nuclear factor-3 PMID: 12215259
11. AFP has a role in female fertility, but not in male fertility or survival, in mice PMID: 12297623
12. mouse and human alpha-fetoprotein enhancers are strikingly different PMID: 15028291
13. AFP protects the developing female brain from the adverse effects of prenatal estrogen exposure and clarify a long-running debate on the role of this fetal protein in brain sexual differentiation PMID: 16479017
14. 30-kDa protein is a novel isoform of AUF1 family and is the main component of the DAP-II complex that binds to the DAS sequence PMID: 16514630
15. The sex difference in the ability to show preovulatory LH surges depends on the prenatal actions of estrogens in the male hypothalamus, is lost in Afp(-/-) females. PMID: 18202134
16. data indicate that this enhancer region is required for alpha-fetoprotein and albumin activation early in liver development and alpha-fetoprotein reactivation during liver regeneration PMID: 19782060
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亞細(xì)胞定位：

Secreted.
蛋白家族：

ALB/AFP/VDB family
組織特異性：

Plasma.
數(shù)據(jù)庫(kù)鏈接：

KEGG: mmu:11576

STRING: 10090.ENSMUSP00000041006

UniGene: Mm.358570