Recombinant Mouse Alpha-actinin-1 (Actn1), partial

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中文名稱：

Recombinant Mouse Alpha-actinin-1 (Actn1), partial
品名簡稱：

Recombinant Mouse Actn1 protein, partial
貨號：

CSB-EP766510MO
說明書：

MSDS Datasheet
規格：

￥1836
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
產品評價
相關產品
靶點詳情

產品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Actn1
Uniprot No.：

Q7TPR4
別名：

Alpha-actinin cytoskeletal isoform;F-actin cross-linking protein;Non-muscle alpha-actinin-1
種屬：

Mus musculus (Mouse)
蛋白長度：

Partial
來源：

E.coli
分子量：

32.0 kDa
表達區域：

31-250aa
氨基酸序列

KQQRKTFTAWCNSHLRKAGTQIENIEEDFRDGLKLMLLLEVISGERLAKPERGKMRVHKISNVNKALDFIASKGVKLVSIGAEEIVDGNVKMTLGMIWTIILRFAIQDISVEETSAKEGLLLWCQRKTAPYKNVNIQNFHISWKDGLGFCALIHRHRPELIDYGKLRKDDPLTNLNTAFDVAERFLDIPKMLDAEDIVGTARPDEKAIMTYVSSFYHAFS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

C-terminal 6xHis-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein.
基因功能參考文獻：
1. The data suggested that Daam1 affected actin assembly during oocyte meiotic division via the regulation of fascin expression. PMID: 28682694
2. These findings evidence alpha-actinin involvement in sarcomeric development, suggesting for this protein an important role in stabilizing the muscle contractile apparatus. PMID: 25262166
3. The C-terminal polybasic region of CYTH2 participates in interacting directly with the EFh2 domain of ACTN1. PMID: 22659138
4. Actn1 differential methylation occurs in diverse mouse tissues. Authors have found no indication of an imprinted effect on gene expression of Actn1 in mice. PMID: 23145029
5. alpha-actinin has a role in adhesion maturation through integrin-dependent force transmission to the extracellular matrix PMID: 23515331
6. Alpha-actinin interaction with syndecan-4 has a role in stabilising cell-matrix adhesion. PMID: 22940199
7. the SRF-cofilin-actin signaling axis modulates neuronal mitochondrial function PMID: 22927399
8. vinculin engagement of adhesion receptors first activates talin or alpha-actinin, by provoking structural changes that allow their vinculin binding sites to swing out, which are then sufficient to bind to and activate vinculin PMID: 16407299
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亞細胞定位：

Cytoplasm, cytoskeleton. Cytoplasm, myofibril, sarcomere, Z line. Cell membrane. Cell junction. Cell projection, ruffle.
蛋白家族：

Alpha-actinin family
數據庫鏈接：

KEGG: mmu:109711

STRING: 10090.ENSMUSP00000021554

UniGene: Mm.253564