Recombinant Mouse Alkylated DNA repair protein alkB homolog 8 (Alkbh8)

中文名稱：

Recombinant Mouse Alkylated DNA repair protein alkB homolog 8(Alkbh8)
貨號：

CSB-YP767352MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Yeast
其他：

中文名稱：

Recombinant Mouse Alkylated DNA repair protein alkB homolog 8(Alkbh8)
貨號：

CSB-EP767352MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
其他：

中文名稱：

Recombinant Mouse Alkylated DNA repair protein alkB homolog 8(Alkbh8)
貨號：

CSB-EP767352MO-B
說明書：

MSDS Datasheet
規(guī)格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

Recombinant Mouse Alkylated DNA repair protein alkB homolog 8(Alkbh8)
貨號：

CSB-BP767352MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Baculovirus
其他：

中文名稱：

Recombinant Mouse Alkylated DNA repair protein alkB homolog 8(Alkbh8)
貨號：

CSB-MP767352MO
說明書：

MSDS Datasheet
規(guī)格：
來源：

Mammalian cell
其他：

產(chǎn)品詳情
產(chǎn)品評價
靶點詳情

產(chǎn)品詳情

純度：

>85% (SDS-PAGE)
基因名：

Alkbh8
Uniprot No.：

Q80Y20
別名：

Alkbh8Alkylated DNA repair protein alkB homolog 8; EC 1.14.11.-; Probable alpha-ketoglutarate-dependent dioxygenase ABH8; S-adenosyl-L-methionine-dependent tRNA methyltransferase ABH8; tRNA; carboxymethyluridine(34)-5-O)-methyltransferase ABH8; EC 2.1.1.-; EC 2.1.1.229
種屬：

Mus musculus (Mouse)
蛋白長度：

full length protein
表達區(qū)域：

1-664
氨基酸序列

MNINHKGVLK LTKMEKKFLR KQSKARHVLL KHEGIQAVSY PTQSLVIANG GLGNGVSRKQ LLLTLEKCGP VEALLMPPNK PYAFVIFQTI EESKKAYFTL NGKEIIDDLG QKIFLYLNFV EKAQWKNMGL EALPPGLLVV EEIISSEEEK KLLESVNWTE DTGNQNFQRS LKHRRVKHFG YEFHYESNTV DKDKPLPGGL PEVCSSILEK LLKEGYIKHK PDQLTINQYE PGHGIPAHID THSAFEDEII SLSLGSAIVM DFKHPEGVTV QVMLPRRSLL VMTGESRYLW THGITPRKFD TVQASEQFKG GIITSDIGDL TLSKRGMRTS FTFRKVRRMP CNCSYSSVCD RQRKATPPSL TESSKEALEL EQKHVHQVYN EIASHFSSTR HSPWPRIVEF LKALPSGSIV ADIGCGNGKY LGINKDLYMI GCDRSQNLVD ICRERQFQAL VCDALAVPVR SGSCDACISI AVIHHFATAE RRVEALQELA RLLRPGGQAL IYVWAMEQEY KNQKSKYLRG KRISQGDKDE LNSATSTEEF LVNQTPEGVN EDPALSVNSS SITKEEEYKS RKVPNSELPI HINRTCFHSQ DVLVPWHLKR NPGKDKAIEP SGVAGCPDPS PVFHRYYHVF CDGELEASCQ AVGDVSILQS YYDQGNWCVV LQKV
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產(chǎn)品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產(chǎn)品評價

靶點詳情

功能：

Catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. Catalyzes the last step in the formation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in target tRNA. Has a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). Binds tRNA and catalyzes the iron and alpha-ketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine; has a preference for tRNA(Gly). Required for normal survival after DNA damage. May inhibit apoptosis and promote cell survival and angiogenesis.
基因功能參考文獻：
1. Alkbh8 and tRNA modifications as central regulators of cellular oxidative stress responses in mammalian systems. PMID: 26147969
2. Many eukaryotic tRNAs contain the wobble modification 5-methoxycarbonylmethyl-uridine (mcm5U). It is demonstrated that (R)- and (S)-5-methoxycarbonylhydroxymethyluridine (mchm5U), hydroxylated forms of mcm(5)U, are present in mammalian tRNA-Arg(UCG), and tRNA-Gly(UCC), respectively. It is shown that the hydroxylation reaction leading to the formation of (S)-mchm5U is catalyzed by the oxygenase (AlkB) domain of ALKBH8. PMID: 21285950
3. The methyltransferase domain of ALKBH8 is demonstrated to be a functional homologue of the Saccharomyces cerevisiae Trm9 protein, mediating the last step in the formation of the wobble uridine modification 5-methoxycarbonylmethyl-uridine in tRNA. This modification is shown to be important for efficient selenoprotein synthesis. ALKBH8 knock-out mice are generated and described. PMID: 20123966
4. The AlkB domain of ALKBH8 specifically hydroxylates mcm(5)U into (S)-mchm(5)U in tRNA-Gly(UCC). PMID: 21285950
5. Data show that ALKBH8 is a tRNA methyltransferase required for the final step in the biogenesis of mcm5U. PMID: 20123966
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亞細胞定位：

Cytoplasm. Nucleus.
蛋白家族：

AlkB family
數(shù)據(jù)庫鏈接：

KEGG: mmu:67667

STRING: 10090.ENSMUSP00000061511

UniGene: Mm.116968