Recombinant Mouse Aldehyde dehydrogenase 1A1 (Aldh1a1)

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中文名稱：

Recombinant Mouse Aldehyde dehydrogenase 1A1(Aldh1a1)
品名簡(jiǎn)稱：

Recombinant Mouse Aldh1a1 protein
貨號(hào)：

CSB-EP001565MO
說明書：

MSDS Datasheet
規(guī)格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產(chǎn)品詳情
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情

產(chǎn)品詳情

純度：

Greater than 95% as determined by SDS-PAGE.
生物活性：

Not Test
基因名：

Aldh1a1
Uniprot No.：

P24549
別名：

3-deoxyglucosone dehydrogenase;ALDH-E1;ALHDII;Aldehyde dehydrogenase family 1 member A1;Aldehyde dehydrogenase, cytosolic;Retinal dehydrogenase 1;RALDH 1;RalDH1
種屬：

Mus musculus (Mouse)
蛋白長(zhǎng)度：

Full Length of Mature Protein
來源：

E.coli
分子量：

58.4 kDa
表達(dá)區(qū)域：

2-501aa
氨基酸序列

SSPAQPAVPAPLADLKIQHTKIFINNEWHNSVSGKKFPVLNPATEEVICHVEEGDKADVDKAVKAARQAFQIGSPWRTMDASERGRLLNKLADLMERDRLLLATMEALNGGKVFANAYLSDLGGCIKALKYCAGWADKIHGQTIPSDGDIFTYTRREPIGVCGQIIPWNFPMLMFIWKIGPALSCGNTVVVKPAEQTPLTALHLASLIKEAGFPPGVVNIVPGYGPTAGAAISSHMDVDKVAFTGSTQVGKLIKEAAGKSNLKRVTLELGGKSPCIVFADADLDIAVEFAHHGVFYHQGQCCVAASRIFVEESVYDEFVKRSVERAKKYVLGNPLTPGINQGPQIDKEQHDKILDLIESGKKEGAKLECGGGRWGNKGFFVQPTVFSNVTDEMRIAKEEIFGPVQQIMKFKSVDDVIKRANNTTYGLAAGLFTKDLDKAITVSSALQAGVVWVNCYMMLSAQCPFGGFKMSGNGRELGEHGLYEYTELKTVAMKISQKNS
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標(biāo)簽：

N-terminal 6xHis-tagged
產(chǎn)品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.
復(fù)溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲(chǔ)存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質(zhì)期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項(xiàng)：

Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.
Datasheet & COA：

Please contact us to get it.

產(chǎn)品評(píng)價(jià)

靶點(diǎn)詳情

功能：

Cytosolic dehydrogenase that catalyzes the irreversible oxidation of a wide range of aldehydes to their corresponding carboxylic acid. Functions downstream of retinol dehydrogenases and catalyzes the oxidation of retinaldehyde into retinoic acid, the second step in the oxidation of retinol/vitamin A into retinoic acid. This pathway is crucial to control the levels of retinol and retinoic acid, two important molecules which excess can be teratogenic and cytotoxic (Probable). Also oxidizes aldehydes resulting from lipid peroxidation like (E)-4-hydroxynon-2-enal/HNE, malonaldehyde and hexanal that form protein adducts and are highly cytotoxic. By participating for instance to the clearance of (E)-4-hydroxynon-2-enal/HNE in the lens epithelium prevents the formation of HNE-protein adducts and lens opacification. Functions also downstream of fructosamine-3-kinase in the fructosamine degradation pathway by catalyzing the oxidation of 3-deoxyglucosone, the carbohydrate product of fructosamine 3-phosphate decomposition, which is itself a potent glycating agent that may react with lysine and arginine side-chains of proteins. Has also an aminobutyraldehyde dehydrogenase activity and is probably part of an alternative pathway for the biosynthesis of GABA/4-aminobutanoate in midbrain, thereby playing a role in GABAergic synaptic transmission.
基因功能參考文獻(xiàn)：
1. Data indicate involvement of non-POU domain-containing octamer-binding protein (NONO)/paraspeckle protein component 1 (PSPC1)-aldehyde dehydrogenase 1 (NONO/PSPC1-ALDH1A1) in the modulation of Sertoli cells (SCs) response to mono-(2-ethylhexyl) phthalate (MEHP) challenge. PMID: 28117896
2. In ovaries lacking ALDH1A1, the onset of germ cell meiosis is delayed. PMID: 26892828
3. Dioxin receptor regulates aldehyde dehydrogenase to block melanoma tumorigenesis and metastasis PMID: 26242870
4. these experiments reveal an evolutionarily conserved GABA synthesis pathway mediated by aldehyde dehydrogenase 1a1 (ALDH1a1). PMID: 26430123
5. Respiratory tract epithelial cells express retinaldehyde dehydrogenase ALDH1A and enhance IgA production by stimulated B cells in the presence of vitamin A. PMID: 24466150
6. Dkk-1 enhances resistance of osteosarcoma cells to stress by tipping balance of Wnt signaling in favor of the non-canonical Jun-mediated Wnt pathways. In turn, this results in transcriptional activation of ALDH1 through Jun-responsive promoter elements. PMID: 24577091
7. Deletion of Aldh1a1 exacerbated alpha-synuclein-mediated DA neurodegeneration and alpha-synuclein aggregation, whereas Aldh1a1-null and control DA neurons were comparably susceptible to 1-methyl-4-phenylpyridinium-, glutamate-, or camptothecin-induced cell death. PMID: 24865427
8. Our findings indicated that increased expression of ALDH1A1 in nasopharyngeal carcinoma was associated with enhanced invasiveness in the nude mouse. PMID: 23625387
9. PSMB8, ALDH1A1, and HSPA4 were identified to be located in ovarian tissues, to regulate 12 cellular pathways, and demonstrate age-dependent dynamic changes in expression profiling. PMID: 24156634
10. beta-escin inhibits tobacco carcinogen-induced lung tumor formation by modulating ALDH1A1-positive cells and RhoA/Rock signaling PMID: 23963803
11. ALDH1A1 deficiency induces BMP2 and increases bone mass in vivo. PMID: 23951127
12. retinoic acid-producing enzyme aldehyde dehydrogenase 1a1 (Aldh1a1) is an oncogene suppressor in specific B cell populations. PMID: 24080087
13. Expression of ALDH1A1 isozyme transcripts was highest in ALDH(very-br) cells, intermediate in ALDH(br) cells, and lowest in ALDH(dim) cells. PMID: 23484127
14. high fat diet mediates visceral fat formation through a sex-specific autocrine Aldh1 switch. PMID: 22933113
15. Retinaldehyde dehydrogenase 1 coordinates hepatic gluconeogenesis and lipid metabolism PMID: 22555438
16. analysis of neurodegeneration and motor dysfunction in mice lacking cytosolic and mitochondrial aldehyde dehydrogenases PMID: 22384032
17. This result demonstrates the critical role of stress resistance in myogenic cell therapy as well as confirms the role of ALDH as a marker for rapid isolation of murine and human myogenic progenitors for cell therapy. PMID: 22195027
18. data establish Aldh1a1 and its substrate Rald as previously unrecognized determinants of adipocyte plasticity and adaptive thermogenesis, which may have potential therapeutic implications PMID: 22561685
19. ALDH1A1 deficiency increases ALDH3A1 expression. Mice deficient in both ALDH3A1 & ALDH1A1 have fewer HSCs, aberrant cell-cycle distribution, increased reactive oxygen species, p38 MAP kinase activity & sensitivity to DNA damage. PMID: 22198153
20. expression can be detected in canals of Hering and bile duct epithelial cells and is increased on liver injury PMID: 21953779
21. in young adult animals with deletion of the Aldh1a1 gene there is altered dopamine metabolism and dysfunction of the dopamine transporter and dopamine release mechanisms PMID: 21784415
22. regulation of fat depots through the concerted action of Aldh1 enzymes establishes retinoic acid-dependent tandem regulation of transcription factors ZFP423 and PPARgamma in a depot-specific manner PMID: 21436255
23. Aldh1a1 plays a major role in cellular defense against oxidative damage induced by reactive lipid aldehydes in mouse liver PMID: 21256123
24. We observed a decrease in the average number of Aldh1a1-positive cells in tumours with a non-invasive vs. solid morphology and in the average number of Aldh1a1-positive mammary tumour cells in low vs. intermediate and low vs. high-nuclear grade tumours. PMID: 21059124
25. Retinoic acid-dependent signaling within the intestine is indispensable for RALDH enzyme activity in the draining mesenteric lymph nodes. PMID: 21220692
26. The addition of all trans retinoic acid blocked DEAB-mediated expansion of ST-HSCs in culture, suggesting that ALDH1a1 regulates HSC differentiation via augmentation of retinoid signaling. PMID: 20054864
27. Three retinaldehyde dehydrogenases (RALDH1, RALDH2 and RALDH3), show differential expression patterns throughout later mouse organogenesis PMID: 11744377
28. RA-generating activities in Raldh2-null mouse embryos could be ascribed to RALDH1 in eye, nasal, and inner ear epithelia. PMID: 12454286
29. RA signaling may be necessary only during early stages of retina development and is catalyzed by multiple enzymes, including Raldh1 PMID: 12808103
30. Raldh1 functions downstream of Adh1 in the oxidative metabolism of excess retinol PMID: 12851412
31. Expression of aldehyde dehydrogenase 1A1 (ALDH1A1) mRNA was found to be higher in brains of DBA/2 mice. PMID: 17010133
32. ALDH1A1 mRNA. 3-deoxyglucosone dehydrogenase activity was undetectable in tissues from ALDH1A1(-/-) mice. PMID: 17175089
33. Cellular expression of Aldh1 in the GI tract was studied. PMID: 17257171
34. corneal ALDH3A1 and lens ALDH1A1 protect the eye against cataract formation via nonenzymatic (light filtering) and enzymatic (detoxification) functions. PMID: 17567582
35. Aldh1a1 is not a critical regulator of adult stem cell function PMID: 18971422
36. the Aldh1a1 gene is strongly expressed in a male-specific manner in somatic cells of the developing testis, beginning shortly after Sry expression is first detectable PMID: 19618468
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亞細(xì)胞定位：

Cytoplasm, cytosol. Cell projection, axon.
蛋白家族：

Aldehyde dehydrogenase family
組織特異性：

Expressed in retina. Expressed in lens and cornea (at protein level). Expressed by midbrain dopamine neurons.
數(shù)據(jù)庫鏈接：

KEGG: mmu:11668

STRING: 10090.ENSMUSP00000084918

UniGene: Mm.250866