Recombinant Mouse Achaete-scute homolog 2 (Ascl2)

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中文名稱：

Recombinant Mouse Achaete-scute homolog 2(Ascl2)
品名簡稱：

Recombinant Mouse Ascl2 protein
貨號：

CSB-EP002200MO
說明書：

MSDS Datasheet
規格：

￥2328
圖片：
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
其他：

產品詳情
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靶點詳情

產品詳情

純度：

Greater than 85% as determined by SDS-PAGE.
基因名：

Ascl2
Uniprot No.：

O35885
別名：

Ascl2; Mash2Achaete-scute homolog 2; ASH-2; mASH-2; mASH2
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length
來源：

E.coli
分子量：

32.8 kDa
表達區域：

1-263aa
氨基酸序列

MEAHLDWYGVPGLQEASDACPRESCSSALPEAREGANVHFPPHPVPREHFSCAAPELVAGAQGLNASLMDGGALPRLMPTSSGVAGACAARRRQASPELLRCSRRRRSGATEASSSSAAVARRNERERNRVKLVNLGFQALRQHVPHGGANKKLSKVETLRSAVEYIRALQRLLAEHDAVRAALAGGLLTPATPPSDECAQPSASPASASLSCASTSPSPDRLGCSEPTSPRSAYSSEESSCEGELSPMEQELLDFSSWLGGY
Note: The complete sequence may include tag sequence, target protein sequence, linker sequence and extra sequence that is translated with the protein sequence for the purpose(s) of secretion, stability, solubility, etc.
If the exact amino acid sequence of this recombinant protein is critical to your application, please explicitly request the full and complete sequence of this protein before ordering.
蛋白標簽：

N-terminal 10xHis-tagged and C-terminal Myc-tagged
產品提供形式：

Liquid or Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
緩沖液：

Tris-based buffer,50% glycerol
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

3-7 business days
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
產品描述：

This recombinant Mouse Ascl2?protein is typically achieved by the manipulation of Ascl2?gene expression in E.coli?cells so that it expresses large amounts of a recombinant Ascl2 gene. In order to get enough amount of the Ascl2?protein, strain selection, codon optimization, fusion systems, co-expression, mutagenesis, and isotope labeling techniques are used. Finally, the Ascl2 protein is isolated from the samples such as cell lysates or medium. Protein refolding, cleavage of fusion moieties and chromatography techniques are involved in the protein purification process.
Ascl2, a basic helix-loop-helix (bHLH) transcription factor, is required for diploid multipotent trophoblast progenitors, intestinal stem cells, follicular T-helper cells, as well as during epidermal development and myogenesis. It is expressed in neuronal precursors. Ascl2 is a downstream target of the Wnt signaling pathway and also a cancer stem cell marker. Ascl2 has been reported to promote cell proliferation and migration in colon cancer and contribute to epithelial-mesenchymal transition (EMT) and promote metastasis in gastric cancer (GC).
Datasheet & COA：

Please contact us to get it.

產品評價

靶點詳情

功能：

AS-C proteins are involved in the determination of the neuronal precursors in the peripheral nervous system and the central nervous system.
基因功能參考文獻：
1. Ascl2 inhibits myogenic differentiation by targeting MRFs and facilitates the generation of postnatal satellite cells. PMID: 27993983
2. overexpression of the imprinted Ascl2 gene has considerable consequences for placental development, specifically for the parietal trophoblast giant cell and spongiotrophoblast lineages both of which express pregnancy-related hormones. PMID: 27542691
3. The ascl2 forms a transcriptional switch that is both Wnt responsive and Wnt dependent to define stem cell identity. PMID: 25620640
4. Ascl2 directly initiates follicular T-helper cell development PMID: 24463518
5. TSSC3 plays an important role in the differentiation from trophoblast stem cell to trophoblast progenitors and/or labyrinth trophoblasts through the TSSC3/PI3K/AKT/MASH2 signaling pathway. PMID: 23071113
6. Elevated expression of Ascl2 in a Wnt signalling dependent manner specifically in the stem cell compartment of the intestine neither increases tumour formation nor diminishes survival in a well established intestinal tumour model, the Apc(min) mouse. PMID: 22138533
7. Ascl2 knockdown results in tumor growth arrest by miRNA-302b-related inhibition of colon cancer progenitor cells PMID: 22384170
8. Partial loss of Ascl2 function affects all three layers of the mature placenta and causes intrauterine growth restriction. PMID: 21238448
9. Mash2 gene is preferentially expressed from the paternal allele. PMID: 7773285
10. Mash2 is a regulator of Krox24, Mob-1, and CXCR4 expression in cultured Schwann cells and negatively regulates their proliferation; a candidate for missing class B bHLH proteins in peripheral nerves PMID: 12196582
11. structure of the Mash2 gene and placental Mash2 expression at different gestational ages PMID: 12493693
12. ascl2 is a putative regulator of proliferation that is overexpressed in intestinal neoplasia PMID: 16568095
13. The combined results from these gain- and loss-of-function experiments imply that Ascl2 controls intestinal stem cell fate. PMID: 19269367
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亞細胞定位：

Nucleus.
數據庫鏈接：

KEGG: mmu:17173

STRING: 10090.ENSMUSP00000009392

UniGene: Mm.196417