Recombinant Mouse 43 kDa receptor-associated protein of the synapse (Rapsn)

中文名稱：

小鼠Rapsn重組蛋白
貨號：

CSB-YP019337MO
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

小鼠Rapsn重組蛋白
貨號：

CSB-EP019337MO
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

小鼠Rapsn重組蛋白
貨號：

CSB-EP019337MO-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

小鼠Rapsn重組蛋白
貨號：

CSB-BP019337MO
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

小鼠Rapsn重組蛋白
貨號：

CSB-MP019337MO
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

Rapsn
Uniprot No.：

P12672
別名：

Rapsn; 43 kDa receptor-associated protein of the synapse; RAPsyn; 43 kDa postsynaptic protein; Acetylcholine receptor-associated 43 kDa protein
種屬：

Mus musculus (Mouse)
蛋白長度：

Full Length of Mature Protein
表達區域：

2-412
氨基酸序列

GQDQTKQQI EKGLQLYQSN QTEKALQVWM KVLEKGSDLV GRFRVLGCLV TAHSEMGRYK EMLKFAVVQI DTARGLEDAD FLLESYLNLA RSNEKLCEFH KTISYCKTCL GLPGTRAGAQ LGGQVSLSMG NAFLGLSLFQ KALESFEKAL RYAHNNDDTM LECRVCCSLG SFYAQVKDYE KALFFPCKAA ELVNDYGKGW SLKYRAMSQY HMAVAYRLLG HLGSAMECCE ESMKIALQHG DRPLQALCLL CFADIHRSRG DLETAFPRYD SAMSIMTEIG NRLGQVHVLL GVAKCWMARK VQDKALDAIE KAQDLAEEVG NKLSQLKLHC LSESIYRSKG LQRELRTHVV RFHECVEETE LYCGLCGESI GERNSRLQAL PCSHIFHLRC LQNNGTRSCP NCRRSSMKPG FV
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

Postsynaptic protein required for clustering of nicotinic acetylcholine receptors (nAChRs) at the neuromuscular junction. It may link the receptor to the underlying postsynaptic cytoskeleton, possibly by direct association with actin or spectrin.
基因功能參考文獻：
1. The RING domain of rapsyn contains E3 ligase activity. Mutation of the RING domain that abolishes the enzyme activity inhibits rapsyn- as well as agrin-induced AChR clustering in heterologous and muscle cells. Further biological and genetic studies support a working model where rapsyn, a classic scaffold protein, serves as an E3 ligase to induce AChR clustering and NMJ formation, possibly by regulation of AChR neddylation PMID: 27839998
2. Molecular modelling, immunoprecipitation and bimolecular fluorescence complementation approaches identify an alpha-helical stretch of rapsyn to be crucial for binding to the dimerisation and docking domain of PKA type I. PMID: 22331361
3. typical anchoring proteins of central excitatory synapses coimmunoprecipitate and colocalize with rapsyn PMID: 22347480
4. alpha-Actinin, rapsyn, and surface AChR form a ternary complex. PMID: 19055765
5. nAChR mobility in plasma membranes of myoblast cells during their differentiation to myotubes in the presence and absence of rapsyn PMID: 20978122
6. These results provide the first experimental evidence that rapsyn is a direct sequence-specific target of Kaiso and delta-catenin. PMID: 15282317
7. Thus, the metabolic half-life of receptors at the synapse may be modulated by local changes in the subsynaptic ratio of rapsyn to AChR. PMID: 15550459
8. Remarkable dynamism of rapsyn underlies stability and maintenance of the postsynaptic scaffold and suggests that the insertion of different postsynaptic proteins may be operating independently. PMID: 17283077
9. Developmental increase in the amount of rapsyn per acetylcholine receptor promotes postsynaptic receptor packing and stability. PMID: 17362913
10. Beta-catenin interacts with rapsyn, a molecule key for acetylcholine receptor clustering in motoneurons at the neuromuscular junction. PMID: 17428970
11. Rapsyn stabilizes acetylcholine receptor clusters at the neuromuscular junction by suppressing calpain activity. PMID: 17640526
12. At the postnatal neuromuscular synapse agrin signaling elevates the expression and targeting of rapsyn to the postsynaptic membrane, thereby packing more AChRs into stable, functionally-important AChR aggregates. PMID: 18506821
13. Wnt/beta-catenin signaling plays a negative role for postsynaptic differentiation at the neuromuscular junction, probably by regulating the expression of synaptic proteins, such as Rapsyn PMID: 18541538
14. Rapsyn interacts with the acetylcholine receptor via an alpha-helical structural motif. Binding at this site likely mediates the critical rapsyn interaction involved in localizing the acetylcholine receptor at the neuromuscular junction. PMID: 19482062
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亞細胞定位：

Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse, postsynaptic cell membrane; Peripheral membrane protein; Cytoplasmic side. Cytoplasm, cytoskeleton. Note=Cytoplasmic surface of postsynaptic membranes.
蛋白家族：

RAPsyn family
數據庫鏈接：

KEGG: mmu:19400

STRING: 10090.ENSMUSP00000054150

UniGene: Mm.1272