Recombinant Listeria monocytogenes serovar 1/2a Listeriolysin O (hly), partial

中文名稱：

單核增生李斯特菌血清1/2ahly重組蛋白
貨號：

CSB-YP320184LPY
說明書：

MSDS Datasheet
規格：
來源：

Yeast
其他：

中文名稱：

單核增生李斯特菌血清1/2ahly重組蛋白
貨號：

CSB-EP320184LPY
說明書：

MSDS Datasheet
規格：
來源：

E.coli
其他：

中文名稱：

單核增生李斯特菌血清1/2ahly重組蛋白
貨號：

CSB-EP320184LPY-B
說明書：

MSDS Datasheet
規格：
來源：

E.coli
共軛：

Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
其他：

中文名稱：

單核增生李斯特菌血清1/2ahly重組蛋白
貨號：

CSB-BP320184LPY
說明書：

MSDS Datasheet
規格：
來源：

Baculovirus
其他：

中文名稱：

單核增生李斯特菌血清1/2ahly重組蛋白
貨號：

CSB-MP320184LPY
說明書：

MSDS Datasheet
規格：
來源：

Mammalian cell
其他：

產品詳情
產品評價
靶點詳情

產品詳情

純度：

>85% (SDS-PAGE)
基因名：

hly
Uniprot No.：

P13128
別名：

hly; hlyA; lisA; lmo0202; Listeriolysin O; LLO; Thiol-activated cytolysin
種屬：

Listeria monocytogenes serovar 1/2a (strain ATCC BAA-679 / EGD-e)
蛋白長度：

Partial
蛋白標簽：

Tag?type?will?be?determined?during?the?manufacturing?process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
產品提供形式：

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
復溶：

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
儲存條件：

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
保質期：

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
貨期：

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
注意事項：

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet ：

Please contact us to get it.

產品評價

靶點詳情

功能：

A cholesterol-dependent pore-forming toxin, which is a major virulence factor required for the escape of bacteria from phagosomal vacuoles and entry into the host cytosol. After binding to target membranes, the protein undergoes a major conformation change, leading to its insertion in the host membrane and formation of an oligomeric pore complex. Listeriolysin O activates mitogen-activated protein (MAP) kinase activity in host cells, most likely as a result of the permeabilization of the host cell membrane. Also induces a proteasome-independent degradation of UBE2I (the SUMO-conjugating enzyme UBC9) and a proteasome-dependent degradation of some sumoylated proteins. Finally, is necessary and sufficient for spacious Listeria-containing phagosomes (SLAPs) formation, suggesting a role for listeriolysin O in promoting L.monocytogenes replication in vacuoles, leading to persistent infection. Recognized by serum from healthy humans exposed to L.monocytogenes as well from patients who have recovered from listeriosis.
基因功能參考文獻：
1. Listeriolysin O -induced down-regulation of Ubc9 is independent of Ubc9-SUMO interaction, however, it may involve phosphorylation signaling. PMID: 28911869
2. During infection, PML undergoes oxidation-mediated multimerization, associates with the nuclear matrix, and becomes de-SUMOylated due to the pore-forming activity of the Listeria toxin listeriolysin O (LLO). PMID: 28074026
3. The current MD simulation work provides insights into the mechanism by which the compounds inhibit LLO at the atomic level and will be useful for the development of new, selective LLO inhibitors. PMID: 25749652
4. Listeria L. monocytogenes induced decrease in hTERT levels is triggered by listeriolysin O (LLO). PMID: 23006849
5. findings show that listeriolysin O and other cholesterol-dependent cytolysins induce K+ efflux through pore formation at the cell membrane PMID: 21482680
6. Data suggest that CFTR transiently increases phagosomal chloride concentration after infection, potentiating LLO pore formation and vacuole lysis. PMID: 21220348
7. These data showed that listeriolysin O-mediated cytoplasmic entry of Listeria monocytogenes could induce the activation of intracellular calcium signaling, which is essential for maturation and secretion of IL-1 alpha in macrophages. PMID: 20194588
8. L. monocytogenes-infected human PBMCs produced IL-1beta, largely depending on an LLO-mediated phagosomal rupture and cathepsin B release, which is sensed by Nlrp3 PMID: 20008285
9. These results reveal the importance of the 91-99 region in the production of a stable and functional LLO. LD(50) determinations in the mouse model suggest a possible link between LLO stability and virulence. PMID: 16622046
10. Listeria monocytogenes-induced caspase-1 activation and interferon-gamma production in mouse macrophages is ascribed mainly to the presence of listeriolysin O. PMID: 18523249
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亞細胞定位：

Secreted. Host membrane; Multi-pass membrane protein. Host cell membrane; Multi-pass membrane protein.
蛋白家族：

Thiol-activated cytolysin family
數據庫鏈接：

KEGG: lmo:lmo0202

STRING: 169963.lmo0202